Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood
Alzheimer’s disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for the poin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/8/564 |
| _version_ | 1797432357907595264 |
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| author | Yibiao Liu Xingyun Liu Mifang Li Qiong Liu Tailin Xu |
| author_facet | Yibiao Liu Xingyun Liu Mifang Li Qiong Liu Tailin Xu |
| author_sort | Yibiao Liu |
| collection | DOAJ |
| description | Alzheimer’s disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for the point-of-care detection of AD biomarkers in the blood. Such a portable platform integrates nanoAu-modified vertical graphene (VG@Au) into a working electrode, which can significantly improve sensitivity and reduce detection limit due to the large specific surface, excellent electrical conductivity, high stability, and good biocompatibility. The tau protein, as an important factor in the course of AD, is selected as a key AD biomarker. The results show that the linear range of this sensing platform is 0.1 pg/mL to 1 ng/mL, with a detection limit of 0.034 pg/mL (S/N = 3), indicating that this portable sensing platform meets the demand for the detection of the tau protein in the blood. This work offers great potential for AD diagnosis and personal healthcare. |
| first_indexed | 2024-03-09T10:00:19Z |
| format | Article |
| id | doaj.art-46af7815743942ffbb9b4d8f87647420 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T10:00:19Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-46af7815743942ffbb9b4d8f876474202023-12-01T23:29:53ZengMDPI AGBiosensors2079-63742022-07-0112856410.3390/bios12080564Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the BloodYibiao Liu0Xingyun Liu1Mifang Li2Qiong Liu3Tailin Xu4Longgang District Central Hospital of Shenzhen, Shenzhen 518116, ChinaSchool of Biomedical Engineering, Health Science Center, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, ChinaLonggang District Central Hospital of Shenzhen, Shenzhen 518116, ChinaSchool of Biomedical Engineering, Health Science Center, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, ChinaSchool of Biomedical Engineering, Health Science Center, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, ChinaAlzheimer’s disease (AD) is a long-term neurodegenerative disease that poses a serious threat to human life and health. It is very important to develop a portable quantitative device for AD diagnosis and personal healthcare. Herein, we develop a portable electrochemical sensing platform for the point-of-care detection of AD biomarkers in the blood. Such a portable platform integrates nanoAu-modified vertical graphene (VG@Au) into a working electrode, which can significantly improve sensitivity and reduce detection limit due to the large specific surface, excellent electrical conductivity, high stability, and good biocompatibility. The tau protein, as an important factor in the course of AD, is selected as a key AD biomarker. The results show that the linear range of this sensing platform is 0.1 pg/mL to 1 ng/mL, with a detection limit of 0.034 pg/mL (S/N = 3), indicating that this portable sensing platform meets the demand for the detection of the tau protein in the blood. This work offers great potential for AD diagnosis and personal healthcare.https://www.mdpi.com/2079-6374/12/8/564portable electrochemical aptasensorAlzheimer’s diseasetau proteinvertical graphene |
| spellingShingle | Yibiao Liu Xingyun Liu Mifang Li Qiong Liu Tailin Xu Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood Biosensors portable electrochemical aptasensor Alzheimer’s disease tau protein vertical graphene |
| title | Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood |
| title_full | Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood |
| title_fullStr | Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood |
| title_full_unstemmed | Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood |
| title_short | Portable Vertical Graphene@Au-Based Electrochemical Aptasensing Platform for Point-of-Care Testing of Tau Protein in the Blood |
| title_sort | portable vertical graphene au based electrochemical aptasensing platform for point of care testing of tau protein in the blood |
| topic | portable electrochemical aptasensor Alzheimer’s disease tau protein vertical graphene |
| url | https://www.mdpi.com/2079-6374/12/8/564 |
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