Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients
Plant-derived compounds and their extracts are known to exhibit chemo preventive (antimicrobial, antioxidant and other) activities. The levels of such chemo preventive compounds vary depending on environmental factors, including the regions where they grow. Described in this study are: (i) a phytoch...
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3364 |
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| author | Vandana Thotathil Naheed Sidiq Ameena Fakhroo Lakshmaiah Sreerama |
| author_facet | Vandana Thotathil Naheed Sidiq Ameena Fakhroo Lakshmaiah Sreerama |
| author_sort | Vandana Thotathil |
| collection | DOAJ |
| description | Plant-derived compounds and their extracts are known to exhibit chemo preventive (antimicrobial, antioxidant and other) activities. The levels of such chemo preventive compounds vary depending on environmental factors, including the regions where they grow. Described in this study are: (i) a phytochemical analysis of the two plants grown in the desert environment of Qatar, viz., <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i>; (ii) the antibacterial, antifungal and antioxidant activities of various solvent extracts of these plants; (iii) a report on the isolation of several pure compounds from these plants. The phytochemical screening indicated the presence of glycosides, tannins, flavonoids, terpenoids, saponins, phenol and anthraquinones in various extracts of each of the plants. Antibacterial and antioxidant activities were studied using agar diffusion and DPPH methods, respectively. The extracts of <i>Anastatica hierochuntica</i> as well as <i>Aerva javanica</i> inhibit the growth of both gram-positive and gram-negative bacterial species. Various extracts of the two plants also exhibited higher or similar antioxidant activities as those of the standard antioxidants, α-tocopherol and ascorbic acid. The extracts of these plants were further purified by HPLC and characterized by IR and NMR techniques. This process has led to identification of β-sitosterol, campesterol and methyl-9-(4-(3,4-dihydroxy-1′-methyl-5′-oxocyclohexyl)-2-hydroxycyclohexyl)nonanoate from <i>Anastatica hierochuntica</i>, and lupenone, betulinic acid, lupeol acetate and persinoside A and B from <i>Aerva javanica</i>. The results reported herein suggests that <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> are potent sources of phytomedicines. |
| first_indexed | 2024-03-11T04:42:26Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:42:26Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-46b0429b282a4c1c87d29e9c79c3c3202023-11-17T20:37:54ZengMDPI AGMolecules1420-30492023-04-01288336410.3390/molecules28083364Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active IngredientsVandana Thotathil0Naheed Sidiq1Ameena Fakhroo2Lakshmaiah Sreerama3Department of Chemistry and Earth Sciences, Chemistry Program, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, QatarDepartment of Chemistry and Earth Sciences, Chemistry Program, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, QatarDepartment of Chemistry and Earth Sciences, Chemistry Program, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, QatarDepartment of Chemistry and Earth Sciences, Chemistry Program, College of Arts and Sciences, Qatar University, Doha P.O. Box 2713, QatarPlant-derived compounds and their extracts are known to exhibit chemo preventive (antimicrobial, antioxidant and other) activities. The levels of such chemo preventive compounds vary depending on environmental factors, including the regions where they grow. Described in this study are: (i) a phytochemical analysis of the two plants grown in the desert environment of Qatar, viz., <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i>; (ii) the antibacterial, antifungal and antioxidant activities of various solvent extracts of these plants; (iii) a report on the isolation of several pure compounds from these plants. The phytochemical screening indicated the presence of glycosides, tannins, flavonoids, terpenoids, saponins, phenol and anthraquinones in various extracts of each of the plants. Antibacterial and antioxidant activities were studied using agar diffusion and DPPH methods, respectively. The extracts of <i>Anastatica hierochuntica</i> as well as <i>Aerva javanica</i> inhibit the growth of both gram-positive and gram-negative bacterial species. Various extracts of the two plants also exhibited higher or similar antioxidant activities as those of the standard antioxidants, α-tocopherol and ascorbic acid. The extracts of these plants were further purified by HPLC and characterized by IR and NMR techniques. This process has led to identification of β-sitosterol, campesterol and methyl-9-(4-(3,4-dihydroxy-1′-methyl-5′-oxocyclohexyl)-2-hydroxycyclohexyl)nonanoate from <i>Anastatica hierochuntica</i>, and lupenone, betulinic acid, lupeol acetate and persinoside A and B from <i>Aerva javanica</i>. The results reported herein suggests that <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> are potent sources of phytomedicines.https://www.mdpi.com/1420-3049/28/8/3364<i>Anastatica hierochuntica</i><i>Aerva javanica</i>phytochemical analysisantibacterial activitiesantifungal activitiesantioxidant activities |
| spellingShingle | Vandana Thotathil Naheed Sidiq Ameena Fakhroo Lakshmaiah Sreerama Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients Molecules <i>Anastatica hierochuntica</i> <i>Aerva javanica</i> phytochemical analysis antibacterial activities antifungal activities antioxidant activities |
| title | Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients |
| title_full | Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients |
| title_fullStr | Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients |
| title_full_unstemmed | Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients |
| title_short | Phytochemical Analysis of <i>Anastatica hierochuntica</i> and <i>Aerva javanica</i> Grown in Qatar: Their Biological Activities and Identification of Some Active Ingredients |
| title_sort | phytochemical analysis of i anastatica hierochuntica i and i aerva javanica i grown in qatar their biological activities and identification of some active ingredients |
| topic | <i>Anastatica hierochuntica</i> <i>Aerva javanica</i> phytochemical analysis antibacterial activities antifungal activities antioxidant activities |
| url | https://www.mdpi.com/1420-3049/28/8/3364 |
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