Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration
Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Abl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15832 |
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| author | Kelley A. Withers Athena Kvamme Christopher E. Youngstrom Rebekah M. Yarvis Rachel Orpano Gabriel P. Simons Erin E. Irish Chi-Lien Cheng |
| author_facet | Kelley A. Withers Athena Kvamme Christopher E. Youngstrom Rebekah M. Yarvis Rachel Orpano Gabriel P. Simons Erin E. Irish Chi-Lien Cheng |
| author_sort | Kelley A. Withers |
| collection | DOAJ |
| description | Growth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (<i>CrTAA2</i>) and a cell proliferation marker gene (<i>CrWOXB</i>). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of <i>CrTAA2</i>. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results. |
| first_indexed | 2024-03-11T11:28:35Z |
| format | Article |
| id | doaj.art-46b22a05df4343d4b224644a0df35583 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:28:35Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-46b22a05df4343d4b224644a0df355832023-11-10T15:05:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124211583210.3390/ijms242115832Auxin Involvement in Ceratopteris Gametophyte Meristem RegenerationKelley A. Withers0Athena Kvamme1Christopher E. Youngstrom2Rebekah M. Yarvis3Rachel Orpano4Gabriel P. Simons5Erin E. Irish6Chi-Lien Cheng7Department of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USADepartment of Biology, University of Iowa, Iowa City, IA 52242, USAGrowth and development of the Ceratopteris hermaphroditic gametophytes are dependent on cell proliferation in the marginal meristem, which when destroyed will regenerate at a new location on the body margin. We established a laser ablation method to destroy a single initial cell in the meristem. Ablation caused the cessation of cell proliferation accompanied by the disappearance of the expression of an auxin synthesis gene (<i>CrTAA2</i>) and a cell proliferation marker gene (<i>CrWOXB</i>). New meristem regeneration occurred within a predictable distance from the original two days post-ablation, signified by cell proliferation and the expression of <i>CrTAA2</i>. Treatment with the naturally occurring auxin indole-3-acetic acid (IAA), synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D), or the transport inhibitor naphthylphthalamic acid (NPA) altered positioning of the original marginal meristem toward the apex of the gametophyte. IAA altered positioning of the regenerated meristem after damaging the original meristem. A model of auxin involvement in the positioning of the marginal meristem in Ceratopteris is presented to encompass these results.https://www.mdpi.com/1422-0067/24/21/15832fern apical cellsauxin gradientlaser ablationWOXBTAA2lateral meristem |
| spellingShingle | Kelley A. Withers Athena Kvamme Christopher E. Youngstrom Rebekah M. Yarvis Rachel Orpano Gabriel P. Simons Erin E. Irish Chi-Lien Cheng Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration International Journal of Molecular Sciences fern apical cells auxin gradient laser ablation WOXB TAA2 lateral meristem |
| title | Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration |
| title_full | Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration |
| title_fullStr | Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration |
| title_full_unstemmed | Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration |
| title_short | Auxin Involvement in Ceratopteris Gametophyte Meristem Regeneration |
| title_sort | auxin involvement in ceratopteris gametophyte meristem regeneration |
| topic | fern apical cells auxin gradient laser ablation WOXB TAA2 lateral meristem |
| url | https://www.mdpi.com/1422-0067/24/21/15832 |
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