Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method
Objective:Pneumocystis jirovecii (P. jirovecii) is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with P. jirovecii pneumonia (PJP) remains a key priority. This study investigated P. jirovecii in patients with lung cancer using the nested-polymerase chain reac...
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| Format: | Article |
| Language: | English |
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Galenos Yayinevi
2022-12-01
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| Series: | Türkiye Parazitoloji Dergisi |
| Subjects: | |
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http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-ipneumocystis-jirovecii-i-in-lung-/57282
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| author | Ahmed Galip Halidi Merhamet Ölçen Esra Gürbüz Abdurrahman Ekici Selahattin Aydemir Hasan Yılmaz |
| author_facet | Ahmed Galip Halidi Merhamet Ölçen Esra Gürbüz Abdurrahman Ekici Selahattin Aydemir Hasan Yılmaz |
| author_sort | Ahmed Galip Halidi |
| collection | DOAJ |
| description | Objective:Pneumocystis jirovecii (P. jirovecii) is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with P. jirovecii pneumonia (PJP) remains a key priority. This study investigated P. jirovecii in patients with lung cancer using the nested-polymerase chain reaction (PCR) method and examined the relationship between P. jirovecii and clinical findings.Methods:The study included 60 patients with lung cancer and 30 patients without lung cancer. The bronchoalveolar lavage (BAL) fluid samples of these 90 individuals were taken for diagnostic purposes in the University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Chest Diseases. Patient information was recorded. After DNA isolation from the BAL fluid samples taken from patients, the nested-PCR protocol for amplification of mtLSUrRNA in P. jirovecii was performed.Results:P. jirovecii DNA was detected in 40 (66.67%) of the lung cancer patients included in the study and in six (20%) patients without lung cancer, that is, in 46 (51.11%) patients. The rate of nested-PCR positivity in the lung cancer group was significantly higher than that in the non-lung cancer group (p=0.0001). Additionally, a statistically significant correlation was found between anorexia and weight loss, fever and sputum P. jirovecii positivity in patients with lung cancer (p<0.005).Conclusion:These findings suggest that lung cancer patients should be evaluated for PJP. |
| first_indexed | 2024-04-10T10:49:48Z |
| format | Article |
| id | doaj.art-46b464ecdce24a2c9761a7ce0d1010af |
| institution | Directory Open Access Journal |
| issn | 1300-6320 2146-3077 |
| language | English |
| last_indexed | 2024-04-10T10:49:48Z |
| publishDate | 2022-12-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Türkiye Parazitoloji Dergisi |
| spelling | doaj.art-46b464ecdce24a2c9761a7ce0d1010af2023-02-15T16:20:10ZengGalenos YayineviTürkiye Parazitoloji Dergisi1300-63202146-30772022-12-0146427628010.4274/tpd.galenos.2022.4415313049054Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR MethodAhmed Galip Halidi0Merhamet Ölçen1Esra Gürbüz2Abdurrahman Ekici3Selahattin Aydemir4Hasan Yılmaz5 Muş Alparslan University, Bulanık Vocational School, Muş, Turkey Şanlıurfa Training and Research Hospital, Clinic of Chest Diseases, Şanlıurfa, Turkey University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Infectious Diseases and Clinical Microbiology, Van, Turkey Van Yüzüncü Yıl University Faculty of Medicine, Department of Parasitology, Van, Turkey Van Yüzüncü Yıl University Faculty of Medicine, Department of Parasitology, Van, Turkey Van Yüzüncü Yıl University Faculty of Medicine, Department of Parasitology, Van, Turkey Objective:Pneumocystis jirovecii (P. jirovecii) is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with P. jirovecii pneumonia (PJP) remains a key priority. This study investigated P. jirovecii in patients with lung cancer using the nested-polymerase chain reaction (PCR) method and examined the relationship between P. jirovecii and clinical findings.Methods:The study included 60 patients with lung cancer and 30 patients without lung cancer. The bronchoalveolar lavage (BAL) fluid samples of these 90 individuals were taken for diagnostic purposes in the University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Chest Diseases. Patient information was recorded. After DNA isolation from the BAL fluid samples taken from patients, the nested-PCR protocol for amplification of mtLSUrRNA in P. jirovecii was performed.Results:P. jirovecii DNA was detected in 40 (66.67%) of the lung cancer patients included in the study and in six (20%) patients without lung cancer, that is, in 46 (51.11%) patients. The rate of nested-PCR positivity in the lung cancer group was significantly higher than that in the non-lung cancer group (p=0.0001). Additionally, a statistically significant correlation was found between anorexia and weight loss, fever and sputum P. jirovecii positivity in patients with lung cancer (p<0.005).Conclusion:These findings suggest that lung cancer patients should be evaluated for PJP. http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-ipneumocystis-jirovecii-i-in-lung-/57282 pneumocystis jiroveciibronchoalveolar lavagenested pcrturkey |
| spellingShingle | Ahmed Galip Halidi Merhamet Ölçen Esra Gürbüz Abdurrahman Ekici Selahattin Aydemir Hasan Yılmaz Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method Türkiye Parazitoloji Dergisi pneumocystis jirovecii bronchoalveolar lavage nested pcr turkey |
| title | Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method |
| title_full | Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method |
| title_fullStr | Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method |
| title_full_unstemmed | Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method |
| title_short | Investigation of Pneumocystis jirovecii in Lung Cancer Patients with the Nested PCR Method |
| title_sort | investigation of pneumocystis jirovecii in lung cancer patients with the nested pcr method |
| topic | pneumocystis jirovecii bronchoalveolar lavage nested pcr turkey |
| url |
http://www.turkiyeparazitolderg.org/archives/archive-detail/article-preview/nvestigation-of-ipneumocystis-jirovecii-i-in-lung-/57282
|
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