Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios
Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios. Methods: R. diobovatum was cultured in a m...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shahid Sadoughi University of Medical Sciences
2019-06-01
|
| Series: | Journal of Food Quality and Hazards Control |
| Subjects: | |
| Online Access: | http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-573-4&slc_lang=en&sid=1 |
| _version_ | 1819042284805554176 |
|---|---|
| author | M. Mirzaie S.S. Saei-Dehkordi D.B. Levin |
| author_facet | M. Mirzaie S.S. Saei-Dehkordi D.B. Levin |
| author_sort | M. Mirzaie |
| collection | DOAJ |
| description | Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios.
Methods: R. diobovatum was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80), which were established by adding different amounts of (NH4)2SO4 (3.78, 1.51, and 0.94 g/l) as the source of nitrogen. High performance liquid chromatography, gas chromatography, and microplate reader were used to determine the glucose concentration, lipid production, and β-carotene concentration, respectively. Data were analyzed using IBM SPSS statistics (v. 24).
Results: Cultures with a C/N ratio of 50 produced the greatest amount of lipids during 120 h pi. However, lipid synthesis in the first 48 h pi was very low for all three C/N ratios. Analyses of the lipid composition revealed that oleic acid and linoleic acid were the dominant (60%) fatty acids. Cultures with a C/N ratio of 50 also produced the greatest amount of β-carotene.
Conclusion: R. diobovatum in the C/N of 50 culture medium resulted in greater concentrations of lipid and β-carotene. Defining the optimum C/N ratio will enable development of optimized bioprocess engineering parameters for scale-up production of lipid and β-carotene in food industries by this yeast species.
DOI: 10.18502/jfqhc.6.2.956 |
| first_indexed | 2024-12-21T09:38:27Z |
| format | Article |
| id | doaj.art-46b6ad9b7c7f4033b8176955696b8ba4 |
| institution | Directory Open Access Journal |
| issn | 2345-685X 2345-6825 |
| language | English |
| last_indexed | 2024-12-21T09:38:27Z |
| publishDate | 2019-06-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Journal of Food Quality and Hazards Control |
| spelling | doaj.art-46b6ad9b7c7f4033b8176955696b8ba42022-12-21T19:08:32ZengShahid Sadoughi University of Medical SciencesJournal of Food Quality and Hazards Control2345-685X2345-68252019-06-01625865Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen RatiosM. Mirzaie0S.S. Saei-Dehkordi1D.B. Levin2 Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Shahrekord, Shahrekord, Iran Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 5V6, Canada Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios. Methods: R. diobovatum was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80), which were established by adding different amounts of (NH4)2SO4 (3.78, 1.51, and 0.94 g/l) as the source of nitrogen. High performance liquid chromatography, gas chromatography, and microplate reader were used to determine the glucose concentration, lipid production, and β-carotene concentration, respectively. Data were analyzed using IBM SPSS statistics (v. 24). Results: Cultures with a C/N ratio of 50 produced the greatest amount of lipids during 120 h pi. However, lipid synthesis in the first 48 h pi was very low for all three C/N ratios. Analyses of the lipid composition revealed that oleic acid and linoleic acid were the dominant (60%) fatty acids. Cultures with a C/N ratio of 50 also produced the greatest amount of β-carotene. Conclusion: R. diobovatum in the C/N of 50 culture medium resulted in greater concentrations of lipid and β-carotene. Defining the optimum C/N ratio will enable development of optimized bioprocess engineering parameters for scale-up production of lipid and β-carotene in food industries by this yeast species. DOI: 10.18502/jfqhc.6.2.956http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-573-4&slc_lang=en&sid=1YeastsLipidsCarotenoidsFood Industry |
| spellingShingle | M. Mirzaie S.S. Saei-Dehkordi D.B. Levin Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios Journal of Food Quality and Hazards Control Yeasts Lipids Carotenoids Food Industry |
| title | Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios |
| title_full | Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios |
| title_fullStr | Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios |
| title_full_unstemmed | Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios |
| title_short | Lipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios |
| title_sort | lipid and β carotene production by rhodosporidium diobovatum cultured with different carbon to nitrogen ratios |
| topic | Yeasts Lipids Carotenoids Food Industry |
| url | http://jfqhc.ssu.ac.ir/browse.php?a_code=A-10-573-4&slc_lang=en&sid=1 |
| work_keys_str_mv | AT mmirzaie lipidandbcaroteneproductionbyrhodosporidiumdiobovatumculturedwithdifferentcarbontonitrogenratios AT sssaeidehkordi lipidandbcaroteneproductionbyrhodosporidiumdiobovatumculturedwithdifferentcarbontonitrogenratios AT dblevin lipidandbcaroteneproductionbyrhodosporidiumdiobovatumculturedwithdifferentcarbontonitrogenratios |