Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles
The purpose of this work was to research the enhancement of Epimedium fried with suet oil based on the in vivo formation self-assembled flavonoid nanomicelles. Taking icariin as the representative, under the action of suet oil, self-assembled nanomicelles were prepared under simulated gastrointestin...
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| Format: | Article |
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MDPI AG
2012-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/11/12984 |
| _version_ | 1818149597764124672 |
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| author | Xiao-Bin Jia Xin Jin Ying-Jie Wei Xiao-Bin Tan Zhen-Hai Zhang Li Cui E Sun |
| author_facet | Xiao-Bin Jia Xin Jin Ying-Jie Wei Xiao-Bin Tan Zhen-Hai Zhang Li Cui E Sun |
| author_sort | Xiao-Bin Jia |
| collection | DOAJ |
| description | The purpose of this work was to research the enhancement of Epimedium fried with suet oil based on the in vivo formation self-assembled flavonoid nanomicelles. Taking icariin as the representative, under the action of suet oil, self-assembled nanomicelles were prepared under simulated gastrointestinal tract conditions and were characterized by dynamic light scattering and transmission electron microscopy (TEM). The experiments with icariin self-assembled nanomicelles without suet oil were done according to the above. The influence of suet oil on the transportation of icariin across Caco-2 cell monolayers and the absorption in rat intestine of self-assembled nanomicelles were evaluated. The particle size of icariin self-assembled nanomicelles with suet oil was smaller than without suet oil. The nanomicelles seemed to be monodisperse spherical particle with smooth surfaces. The icariin entrapment efficiency of self-assembled nanomicelles with suet oil was increased from 43.1% to 89.7%. In Caco-2 cell monolayers, the absorptive permeability, secretory permeability and efflux ratio of icariin self-assembled nanomicelles with suet oil was 1.26 × 10−6 cm/s, 5.91 × 10−6 cm/s and 4.69, respectively, while that of icariin self-assembled nanomicelles without suet oil was 0.62 × 10−6 cm/s, 3.00 × 10−6 cm/s, and 4.84, respectively. In rat intestinal perfusion experiments, the permeability coefficient of icariin self-assembled nanomicelles with suet oil in duodenum was higher than the value of icariin self-assembled nanomicelles without suet oil (p < 0.05). With the action of suet oil, icariin self-assembled nanomicelles were more stable and the entrapment efficiency was higher than that without suet oil, which could increase the solubility of icariin and improve its intestinal absorption. Therefore, suet oil plays a role in its enhancement. |
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| id | doaj.art-46bc79670572408f8c1452d6d0d4a718 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T13:09:34Z |
| publishDate | 2012-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-46bc79670572408f8c1452d6d0d4a7182022-12-22T01:06:13ZengMDPI AGMolecules1420-30492012-11-011711129841299610.3390/molecules171112984Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound NanomicellesXiao-Bin JiaXin JinYing-Jie WeiXiao-Bin TanZhen-Hai ZhangLi CuiE SunThe purpose of this work was to research the enhancement of Epimedium fried with suet oil based on the in vivo formation self-assembled flavonoid nanomicelles. Taking icariin as the representative, under the action of suet oil, self-assembled nanomicelles were prepared under simulated gastrointestinal tract conditions and were characterized by dynamic light scattering and transmission electron microscopy (TEM). The experiments with icariin self-assembled nanomicelles without suet oil were done according to the above. The influence of suet oil on the transportation of icariin across Caco-2 cell monolayers and the absorption in rat intestine of self-assembled nanomicelles were evaluated. The particle size of icariin self-assembled nanomicelles with suet oil was smaller than without suet oil. The nanomicelles seemed to be monodisperse spherical particle with smooth surfaces. The icariin entrapment efficiency of self-assembled nanomicelles with suet oil was increased from 43.1% to 89.7%. In Caco-2 cell monolayers, the absorptive permeability, secretory permeability and efflux ratio of icariin self-assembled nanomicelles with suet oil was 1.26 × 10−6 cm/s, 5.91 × 10−6 cm/s and 4.69, respectively, while that of icariin self-assembled nanomicelles without suet oil was 0.62 × 10−6 cm/s, 3.00 × 10−6 cm/s, and 4.84, respectively. In rat intestinal perfusion experiments, the permeability coefficient of icariin self-assembled nanomicelles with suet oil in duodenum was higher than the value of icariin self-assembled nanomicelles without suet oil (p < 0.05). With the action of suet oil, icariin self-assembled nanomicelles were more stable and the entrapment efficiency was higher than that without suet oil, which could increase the solubility of icariin and improve its intestinal absorption. Therefore, suet oil plays a role in its enhancement.http://www.mdpi.com/1420-3049/17/11/12984Epimediumsuet oilself-assembled nanomicellesicariinCaco-2 cell monolayerssingle-pass rat intestinal perfusion |
| spellingShingle | Xiao-Bin Jia Xin Jin Ying-Jie Wei Xiao-Bin Tan Zhen-Hai Zhang Li Cui E Sun Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles Molecules Epimedium suet oil self-assembled nanomicelles icariin Caco-2 cell monolayers single-pass rat intestinal perfusion |
| title | Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles |
| title_full | Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles |
| title_fullStr | Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles |
| title_full_unstemmed | Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles |
| title_short | Enhancement of Epimedium Fried with Suet Oil Based on in Vivo Formation of Self-Assembled Flavonoid Compound Nanomicelles |
| title_sort | enhancement of epimedium fried with suet oil based on in vivo formation of self assembled flavonoid compound nanomicelles |
| topic | Epimedium suet oil self-assembled nanomicelles icariin Caco-2 cell monolayers single-pass rat intestinal perfusion |
| url | http://www.mdpi.com/1420-3049/17/11/12984 |
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