Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model
IntroductionExaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, the contributions of bacterial communities are less defined...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2023.1113043/full |
| _version_ | 1811167106667905024 |
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| author | Ezra Curtis Joanne Moseley Riccardo Racicot Rachel M. Wright Rachel M. Wright |
| author_facet | Ezra Curtis Joanne Moseley Riccardo Racicot Rachel M. Wright Rachel M. Wright |
| author_sort | Ezra Curtis |
| collection | DOAJ |
| description | IntroductionExaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, the contributions of bacterial communities are less defined in this system.MethodsWe investigated microbial variation between distinct parts of the body and symbiotic state across four genets held in identical environmental conditions using 16s rRNA gene amplicon sequencing.ResultsWe found differentially abundant taxa between body part and symbiotic state that highlight the roles these bacteria may play in holobiont heterotrophy and nutrient cycling. Beta-diversity analysis revealed distinct communities between symbiotic states consistent with previous studies; however, we did not observe the presence of previously reported core microbiota. We also found community differences across clonal lines, despite years of identical rearing conditions. ConclusionThese findings suggest the Exaiptasia bacterial microbiome is greatly influenced by host genetics and unpredictable environmental influences. |
| first_indexed | 2024-04-10T16:03:31Z |
| format | Article |
| id | doaj.art-46bc97482bcb4107afcad218a319afbb |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-10T16:03:31Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-46bc97482bcb4107afcad218a319afbb2023-02-10T06:47:49ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452023-02-011010.3389/fmars.2023.11130431113043Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian modelEzra Curtis0Joanne Moseley1Riccardo Racicot2Rachel M. Wright3Rachel M. Wright4Department of Biological Sciences, Smith College, Northampton, MA, United StatesDepartment of Biological Sciences, Smith College, Northampton, MA, United StatesDepartment of Biological Sciences, Smith College, Northampton, MA, United StatesDepartment of Biological Sciences, Smith College, Northampton, MA, United StatesDepartment of Biological Sciences, Southern Methodist University, Dallas, TX, United StatesIntroductionExaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, the contributions of bacterial communities are less defined in this system.MethodsWe investigated microbial variation between distinct parts of the body and symbiotic state across four genets held in identical environmental conditions using 16s rRNA gene amplicon sequencing.ResultsWe found differentially abundant taxa between body part and symbiotic state that highlight the roles these bacteria may play in holobiont heterotrophy and nutrient cycling. Beta-diversity analysis revealed distinct communities between symbiotic states consistent with previous studies; however, we did not observe the presence of previously reported core microbiota. We also found community differences across clonal lines, despite years of identical rearing conditions. ConclusionThese findings suggest the Exaiptasia bacterial microbiome is greatly influenced by host genetics and unpredictable environmental influences.https://www.frontiersin.org/articles/10.3389/fmars.2023.1113043/fullAiptasiaExaiptasiasymbiosismicrobial communitycoral reef |
| spellingShingle | Ezra Curtis Joanne Moseley Riccardo Racicot Rachel M. Wright Rachel M. Wright Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model Frontiers in Marine Science Aiptasia Exaiptasia symbiosis microbial community coral reef |
| title | Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| title_full | Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| title_fullStr | Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| title_full_unstemmed | Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| title_short | Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| title_sort | bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model |
| topic | Aiptasia Exaiptasia symbiosis microbial community coral reef |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2023.1113043/full |
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