MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and cul...
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Format: | Article |
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PAGEPress Publications
2011-01-01
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Series: | Italian Journal of Food Safety |
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Online Access: | http://www.pagepressjournals.org/index.php/ijfs/article/view/892 |
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author | A. Petruzzelli F. Ciarrocchi M. Foglini S. Fisichella C. Sebastiani W. Baffone S. Santarelli F. Tonucci G. Blasi |
author_facet | A. Petruzzelli F. Ciarrocchi M. Foglini S. Fisichella C. Sebastiani W. Baffone S. Santarelli F. Tonucci G. Blasi |
author_sort | A. Petruzzelli |
collection | DOAJ |
description | The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level. |
first_indexed | 2024-12-11T22:41:12Z |
format | Article |
id | doaj.art-46bd426bf1ac4bf1a41b861263530353 |
institution | Directory Open Access Journal |
issn | 2239-7132 |
language | English |
last_indexed | 2024-12-11T22:41:12Z |
publishDate | 2011-01-01 |
publisher | PAGEPress Publications |
record_format | Article |
series | Italian Journal of Food Safety |
spelling | doaj.art-46bd426bf1ac4bf1a41b8612635303532022-12-22T00:47:47ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322011-01-0111zero28728810.4081/ijfs.2011.1S.287532MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDYA. Petruzzelli0F. Ciarrocchi1M. Foglini2S. Fisichella3C. Sebastiani4W. Baffone5S. Santarelli6F. Tonucci7G. Blasi8Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaDipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – UrbinoIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaDipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino.Dipartimento SAIFET, Università Politecnica delle Marche – AnconaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia.Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia.The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.http://www.pagepressjournals.org/index.php/ijfs/article/view/892Ciauscolo, autochthonous bacteria, RAPD. |
spellingShingle | A. Petruzzelli F. Ciarrocchi M. Foglini S. Fisichella C. Sebastiani W. Baffone S. Santarelli F. Tonucci G. Blasi MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY Italian Journal of Food Safety Ciauscolo, autochthonous bacteria, RAPD. |
title | MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY |
title_full | MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY |
title_fullStr | MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY |
title_full_unstemmed | MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY |
title_short | MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY |
title_sort | molecular identification and genotyping of ciauscolo autochthonous microflora preliminary study |
topic | Ciauscolo, autochthonous bacteria, RAPD. |
url | http://www.pagepressjournals.org/index.php/ijfs/article/view/892 |
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