MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY

The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and cul...

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Main Authors: A. Petruzzelli, F. Ciarrocchi, M. Foglini, S. Fisichella, C. Sebastiani, W. Baffone, S. Santarelli, F. Tonucci, G. Blasi
Format: Article
Language:English
Published: PAGEPress Publications 2011-01-01
Series:Italian Journal of Food Safety
Subjects:
Online Access:http://www.pagepressjournals.org/index.php/ijfs/article/view/892
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author A. Petruzzelli
F. Ciarrocchi
M. Foglini
S. Fisichella
C. Sebastiani
W. Baffone
S. Santarelli
F. Tonucci
G. Blasi
author_facet A. Petruzzelli
F. Ciarrocchi
M. Foglini
S. Fisichella
C. Sebastiani
W. Baffone
S. Santarelli
F. Tonucci
G. Blasi
author_sort A. Petruzzelli
collection DOAJ
description The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.
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spelling doaj.art-46bd426bf1ac4bf1a41b8612635303532022-12-22T00:47:47ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322011-01-0111zero28728810.4081/ijfs.2011.1S.287532MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDYA. Petruzzelli0F. Ciarrocchi1M. Foglini2S. Fisichella3C. Sebastiani4W. Baffone5S. Santarelli6F. Tonucci7G. Blasi8Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaDipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – UrbinoIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – PerugiaDipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino.Dipartimento SAIFET, Università Politecnica delle Marche – AnconaIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia.Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia.The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.http://www.pagepressjournals.org/index.php/ijfs/article/view/892Ciauscolo, autochthonous bacteria, RAPD.
spellingShingle A. Petruzzelli
F. Ciarrocchi
M. Foglini
S. Fisichella
C. Sebastiani
W. Baffone
S. Santarelli
F. Tonucci
G. Blasi
MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
Italian Journal of Food Safety
Ciauscolo, autochthonous bacteria, RAPD.
title MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
title_full MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
title_fullStr MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
title_full_unstemmed MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
title_short MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY
title_sort molecular identification and genotyping of ciauscolo autochthonous microflora preliminary study
topic Ciauscolo, autochthonous bacteria, RAPD.
url http://www.pagepressjournals.org/index.php/ijfs/article/view/892
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