Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma
Objective To investigate the role of miR-155 in the development and lung metastasis of osteosarcoma, and to explore its target proteins and related mechanisms. Methods We detected differential miR-155 expression in osteosarcoma and its lung metastasis process through relevant database analysis combi...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2021-12-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2021.21.0608 |
| _version_ | 1798034177540489216 |
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| author | YANG Huajie LI Lan LI Bin ZHANG Dong ZHANG Qiang |
| author_facet | YANG Huajie LI Lan LI Bin ZHANG Dong ZHANG Qiang |
| author_sort | YANG Huajie |
| collection | DOAJ |
| description | Objective To investigate the role of miR-155 in the development and lung metastasis of osteosarcoma, and to explore its target proteins and related mechanisms. Methods We detected differential miR-155 expression in osteosarcoma and its lung metastasis process through relevant database analysis combined with qPCR detection of clinical tissue and cell samples. iTRAQ quantitative proteomics was used to screen the target protein of miR-155 in osteosarcoma and its lung metastasis. The selected miR-155 target protein was verified by Western blot in clinical tissue and cell samples. Results miR-155 expression was significantly higher in osteosarcoma tissue and lung metastatic tumor tissue than that in the control group. Compared with human normal osteoblasts, miR-155 expression in human osteosarcoma cell line was also significantly increased. A total of 3714 proteins were obtained by iTRAQ assay, and 253 differentially-expressed proteins were screened out, 144 of which were up-regulated and 109 were down-regulated. The prediction analysis and experiment verified that C/EBP β was a potential target protein of miR-155. Conclusion miR-155 expression is significantly increased in osteosarcoma and its lung metastasis process, and C/EBP β is a potential target of miR-155. |
| first_indexed | 2024-04-11T20:40:34Z |
| format | Article |
| id | doaj.art-46be0f26ab22442897f52b1f431249b7 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 |
| language | zho |
| last_indexed | 2024-04-11T20:40:34Z |
| publishDate | 2021-12-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-46be0f26ab22442897f52b1f431249b72022-12-22T04:04:13ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85782021-12-0148121087109510.3971/j.issn.1000-8578.2021.21.06088578.2021.21.0608Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of OsteosarcomaYANG Huajie0LI Lan1LI Bin2ZHANG Dong3ZHANG Qiang4Department of Nursing, Beijing Jishuitan Hospital, Beijing 100035, ChinaDepartment of Pathology, Beijing Jishuitan Hospital, Beijing 100035, ChinaAnimal Experimental Center, Fuwai Hospital, Beijing 100037, ChinaDepartment of Thoracic Surgery, Beijing Jishuitan Hospital, Beijing 100035, ChinaDepartment of Thoracic Surgery, Beijing Jishuitan Hospital, Beijing 100035, ChinaObjective To investigate the role of miR-155 in the development and lung metastasis of osteosarcoma, and to explore its target proteins and related mechanisms. Methods We detected differential miR-155 expression in osteosarcoma and its lung metastasis process through relevant database analysis combined with qPCR detection of clinical tissue and cell samples. iTRAQ quantitative proteomics was used to screen the target protein of miR-155 in osteosarcoma and its lung metastasis. The selected miR-155 target protein was verified by Western blot in clinical tissue and cell samples. Results miR-155 expression was significantly higher in osteosarcoma tissue and lung metastatic tumor tissue than that in the control group. Compared with human normal osteoblasts, miR-155 expression in human osteosarcoma cell line was also significantly increased. A total of 3714 proteins were obtained by iTRAQ assay, and 253 differentially-expressed proteins were screened out, 144 of which were up-regulated and 109 were down-regulated. The prediction analysis and experiment verified that C/EBP β was a potential target protein of miR-155. Conclusion miR-155 expression is significantly increased in osteosarcoma and its lung metastasis process, and C/EBP β is a potential target of miR-155.http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2021.21.0608mir-155osteosarcomapulmonary metastasisproteomicsc/ebp β |
| spellingShingle | YANG Huajie LI Lan LI Bin ZHANG Dong ZHANG Qiang Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma Zhongliu Fangzhi Yanjiu mir-155 osteosarcoma pulmonary metastasis proteomics c/ebp β |
| title | Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma |
| title_full | Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma |
| title_fullStr | Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma |
| title_full_unstemmed | Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma |
| title_short | Role and Mechanism of miRNA-155 in Development, Progression and Lung Metastasis of Osteosarcoma |
| title_sort | role and mechanism of mirna 155 in development progression and lung metastasis of osteosarcoma |
| topic | mir-155 osteosarcoma pulmonary metastasis proteomics c/ebp β |
| url | http://www.zlfzyj.com/EN/10.3971/j.issn.1000-8578.2021.21.0608 |
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