Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune
Schizophyllum commune is a filamentous basidiomycete causing white-rot in many wood species with the help of a broad range of enzymes including multicopper oxidases such as laccases and laccase-like oxidases. Since these enzymes exhibit a broad substrate range, their ability to oxidatively degrade b...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02545/full |
| _version_ | 1818695462509608960 |
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| author | Julia Kirtzel Soumya Madhavan Natalie Wielsch Alexander Blinne Yvonne Hupfer Jörg Linde Katrin Krause Aleš Svatoš Erika Kothe |
| author_facet | Julia Kirtzel Soumya Madhavan Natalie Wielsch Alexander Blinne Yvonne Hupfer Jörg Linde Katrin Krause Aleš Svatoš Erika Kothe |
| author_sort | Julia Kirtzel |
| collection | DOAJ |
| description | Schizophyllum commune is a filamentous basidiomycete causing white-rot in many wood species with the help of a broad range of enzymes including multicopper oxidases such as laccases and laccase-like oxidases. Since these enzymes exhibit a broad substrate range, their ability to oxidatively degrade black slate was investigated. Both haploid monokaryotic, and mated dikaryotic strains were able to grow on black slate rich in organic carbon as sole carbon source. On defined media, only the monokaryon showed growth promotion by addition of slate. At the same time, metals were released from the slate and, after reaching a threshold concentration, inhibited further growth of the fungus. The proteome during decomposition of the black slate showed induction of proteins potentially involved in rock degradation and stress resistance, and the gene for laccase-like oxidase mco2 was up-regulated. Specifically in the dikaryon, the laccase gene lcc1 was induced, while lcc2 as well as mco1, mco3, and mco4 expression levels remained similar. Spectrophotometric analysis revealed that both life forms were able to degrade the rock and produce smaller particles. |
| first_indexed | 2024-12-17T13:45:51Z |
| format | Article |
| id | doaj.art-46c0a0ee263c43d386794e13c9d90b05 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-17T13:45:51Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-46c0a0ee263c43d386794e13c9d90b052022-12-21T21:46:09ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02545355839Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum communeJulia Kirtzel0Soumya Madhavan1Natalie Wielsch2Alexander Blinne3Yvonne Hupfer4Jörg Linde5Katrin Krause6Aleš Svatoš7Erika Kothe8Microbial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyMicrobial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyResearch Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology, Jena, GermanyHelmholtz Institute Jena, Jena, GermanyResearch Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology, Jena, GermanyHans Knöll Institute, Jena, GermanyMicrobial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanyResearch Group Mass Spectrometry/Proteomics, Max Planck Institute for Chemical Ecology, Jena, GermanyMicrobial Communication, Institute of Microbiology, Friedrich Schiller University, Jena, GermanySchizophyllum commune is a filamentous basidiomycete causing white-rot in many wood species with the help of a broad range of enzymes including multicopper oxidases such as laccases and laccase-like oxidases. Since these enzymes exhibit a broad substrate range, their ability to oxidatively degrade black slate was investigated. Both haploid monokaryotic, and mated dikaryotic strains were able to grow on black slate rich in organic carbon as sole carbon source. On defined media, only the monokaryon showed growth promotion by addition of slate. At the same time, metals were released from the slate and, after reaching a threshold concentration, inhibited further growth of the fungus. The proteome during decomposition of the black slate showed induction of proteins potentially involved in rock degradation and stress resistance, and the gene for laccase-like oxidase mco2 was up-regulated. Specifically in the dikaryon, the laccase gene lcc1 was induced, while lcc2 as well as mco1, mco3, and mco4 expression levels remained similar. Spectrophotometric analysis revealed that both life forms were able to degrade the rock and produce smaller particles.https://www.frontiersin.org/article/10.3389/fmicb.2018.02545/fullSchizophyllum communebioweatheringproteomelaccasesmulticopper oxidasesrock |
| spellingShingle | Julia Kirtzel Soumya Madhavan Natalie Wielsch Alexander Blinne Yvonne Hupfer Jörg Linde Katrin Krause Aleš Svatoš Erika Kothe Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune Frontiers in Microbiology Schizophyllum commune bioweathering proteome laccases multicopper oxidases rock |
| title | Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune |
| title_full | Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune |
| title_fullStr | Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune |
| title_full_unstemmed | Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune |
| title_short | Enzymatic Bioweathering and Metal Mobilization From Black Slate by the Basidiomycete Schizophyllum commune |
| title_sort | enzymatic bioweathering and metal mobilization from black slate by the basidiomycete schizophyllum commune |
| topic | Schizophyllum commune bioweathering proteome laccases multicopper oxidases rock |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02545/full |
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