Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
Fruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/7/11/467 |
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| author | Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li |
| author_facet | Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li |
| author_sort | Xinxin Feng |
| collection | DOAJ |
| description | Fruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits (HFs) of ‘Suli’ pear (<i>Pyrus bretschneideri</i> Rehd) was used to examine the molecular mechanism of fruit hardening. We found that hardened fruits (HFs), especially those in the top region within 35–180 days after flowering (DAF), possessed a prominently higher lignin level than normal fruits (NFs). Subsequently, transcriptome sequencing of fruit at 35 DAF identified 4391 [HF shoulder region (HF_S) vs. NF top region (NF_T)], 3849 (HF_T vs. NF_T) and 408 (HF_T vs. HF_S) differentially expressed genes. Among them, we clarified 26 unigenes that encode 10 enzymes as candidate genes participating in lignin biosynthesis. Thus, high expression of the lignin biosynthetic gene impels lignin to accumulate in HFs. Some transcription factors were evaluated to link with lignin formation and subsequently analysed. In addition, the genes participating in flavonoid/proanthocyanidin biosynthesis dropped from the HF_T to the HF_S, suggesting that the flavonoid metabolic pathway was reduced to promote intermediate metabolites to be converted to the lignin biosynthetic pathway. To conclude, this study lays a solid theoretical foundation and provides reference data for investigating the mechanism by which lignin accumulates in hardened pear fruit. |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T05:26:28Z |
| publishDate | 2021-11-01 |
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| series | Horticulturae |
| spelling | doaj.art-46c3c9d491d44c4fa4224bdcdc6e710f2023-11-22T23:34:07ZengMDPI AGHorticulturae2311-75242021-11-0171146710.3390/horticulturae7110467Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and AccumulationXinxin Feng0Xiaofeng Liu1Yuxiu Jia2Huifang Liu3Liulin Li4College of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaFruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits (HFs) of ‘Suli’ pear (<i>Pyrus bretschneideri</i> Rehd) was used to examine the molecular mechanism of fruit hardening. We found that hardened fruits (HFs), especially those in the top region within 35–180 days after flowering (DAF), possessed a prominently higher lignin level than normal fruits (NFs). Subsequently, transcriptome sequencing of fruit at 35 DAF identified 4391 [HF shoulder region (HF_S) vs. NF top region (NF_T)], 3849 (HF_T vs. NF_T) and 408 (HF_T vs. HF_S) differentially expressed genes. Among them, we clarified 26 unigenes that encode 10 enzymes as candidate genes participating in lignin biosynthesis. Thus, high expression of the lignin biosynthetic gene impels lignin to accumulate in HFs. Some transcription factors were evaluated to link with lignin formation and subsequently analysed. In addition, the genes participating in flavonoid/proanthocyanidin biosynthesis dropped from the HF_T to the HF_S, suggesting that the flavonoid metabolic pathway was reduced to promote intermediate metabolites to be converted to the lignin biosynthetic pathway. To conclude, this study lays a solid theoretical foundation and provides reference data for investigating the mechanism by which lignin accumulates in hardened pear fruit.https://www.mdpi.com/2311-7524/7/11/467‘Suli’ pearshardened fruitlignin biosynthesistranscriptomic analysis |
| spellingShingle | Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation Horticulturae ‘Suli’ pears hardened fruit lignin biosynthesis transcriptomic analysis |
| title | Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation |
| title_full | Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation |
| title_fullStr | Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation |
| title_full_unstemmed | Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation |
| title_short | Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation |
| title_sort | transcriptomic analysis of hardened suli pear i pyrus bretschneideri i rehd for identification of key genes for lignin biosynthesis and accumulation |
| topic | ‘Suli’ pears hardened fruit lignin biosynthesis transcriptomic analysis |
| url | https://www.mdpi.com/2311-7524/7/11/467 |
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