Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?
Urinary tract infections (UTIs) are a serious health problem in the human population due to their chronic and recurrent nature. Bacteria causing UTIs form multispecies biofilms being resistant to the activity of the conventionally used antibiotics. Therefore, compounds of plant origin are currently...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Biomolecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-273X/11/12/1754 |
| _version_ | 1797506437086183424 |
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| author | Zuzanna Sycz Dorota Tichaczek-Goska Anna Jezierska-Domaradzka Dorota Wojnicz |
| author_facet | Zuzanna Sycz Dorota Tichaczek-Goska Anna Jezierska-Domaradzka Dorota Wojnicz |
| author_sort | Zuzanna Sycz |
| collection | DOAJ |
| description | Urinary tract infections (UTIs) are a serious health problem in the human population due to their chronic and recurrent nature. Bacteria causing UTIs form multispecies biofilms being resistant to the activity of the conventionally used antibiotics. Therefore, compounds of plant origin are currently being searched for, which could constitute an alternative strategy to antibiotic therapy. Our study aimed to determine the activity of asiatic acid (AA) against biofilms formed by uropathogenic <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Pseudomonas aeruginosa</i>. The influence of AA on the survival, biofilm mass formation by bacteria living in mono-, dual-, and triple-species consortia as well as the metabolic activity and bacterial cell morphology were determined. The spectrophotometric methods were used for biofilm mass synthesis and metabolic activity determination. The survival of bacteria was established using the serial dilution assay. The decrease in survival and a weakening of the ability to create biofilms, both single and multi-species, as well as changes in the morphology of bacterial cells were noticed. As AA works best against young biofilms, the use of AA-containing formulations, especially during the initial stages of infection, seems to be reasonable. However, there is a need for further research concerning AA especially regarding its antibacterial mechanisms of action. |
| first_indexed | 2024-03-10T04:33:36Z |
| format | Article |
| id | doaj.art-46c7f4fd13374b028270a9697eb03356 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T04:33:36Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-46c7f4fd13374b028270a9697eb033562023-11-23T03:58:38ZengMDPI AGBiomolecules2218-273X2021-11-011112175410.3390/biom11121754Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid?Zuzanna Sycz0Dorota Tichaczek-Goska1Anna Jezierska-Domaradzka2Dorota Wojnicz3Department of Biology and Medical Parasitology, Wroclaw Medical University, 50-345 Wroclaw, PolandDepartment of Biology and Medical Parasitology, Wroclaw Medical University, 50-345 Wroclaw, Poland Department of Pharmaceutical Biology and Botany, Wroclaw Medical University, 50-556 Wroclaw, PolandDepartment of Biology and Medical Parasitology, Wroclaw Medical University, 50-345 Wroclaw, PolandUrinary tract infections (UTIs) are a serious health problem in the human population due to their chronic and recurrent nature. Bacteria causing UTIs form multispecies biofilms being resistant to the activity of the conventionally used antibiotics. Therefore, compounds of plant origin are currently being searched for, which could constitute an alternative strategy to antibiotic therapy. Our study aimed to determine the activity of asiatic acid (AA) against biofilms formed by uropathogenic <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Pseudomonas aeruginosa</i>. The influence of AA on the survival, biofilm mass formation by bacteria living in mono-, dual-, and triple-species consortia as well as the metabolic activity and bacterial cell morphology were determined. The spectrophotometric methods were used for biofilm mass synthesis and metabolic activity determination. The survival of bacteria was established using the serial dilution assay. The decrease in survival and a weakening of the ability to create biofilms, both single and multi-species, as well as changes in the morphology of bacterial cells were noticed. As AA works best against young biofilms, the use of AA-containing formulations, especially during the initial stages of infection, seems to be reasonable. However, there is a need for further research concerning AA especially regarding its antibacterial mechanisms of action.https://www.mdpi.com/2218-273X/11/12/1754asiatic acidplant compoundsuropathogenic bacteriaurinary tract infection (UTI)multispecies biofilm |
| spellingShingle | Zuzanna Sycz Dorota Tichaczek-Goska Anna Jezierska-Domaradzka Dorota Wojnicz Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? Biomolecules asiatic acid plant compounds uropathogenic bacteria urinary tract infection (UTI) multispecies biofilm |
| title | Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? |
| title_full | Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? |
| title_fullStr | Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? |
| title_full_unstemmed | Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? |
| title_short | Are Uropathogenic Bacteria Living in Multispecies Biofilm Susceptible to Active Plant Ingredient—Asiatic Acid? |
| title_sort | are uropathogenic bacteria living in multispecies biofilm susceptible to active plant ingredient asiatic acid |
| topic | asiatic acid plant compounds uropathogenic bacteria urinary tract infection (UTI) multispecies biofilm |
| url | https://www.mdpi.com/2218-273X/11/12/1754 |
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