Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study
A sensitive and reliable method based on liquid chromatography tandem mass spectrometry (LC–MS/MS) for the quantitation of cyclovirobuxine D in human plasma has been developed and validated. Sample preparation by solid phase extraction was followed by separation on a CN column with a mobile phase of...
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| Format: | Article |
| Language: | English |
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Elsevier
2011-10-01
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| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383511000633 |
| _version_ | 1818359132090007552 |
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| author | Ling-li Mu Peng Yu Li-hua He |
| author_facet | Ling-li Mu Peng Yu Li-hua He |
| author_sort | Ling-li Mu |
| collection | DOAJ |
| description | A sensitive and reliable method based on liquid chromatography tandem mass spectrometry (LC–MS/MS) for the quantitation of cyclovirobuxine D in human plasma has been developed and validated. Sample preparation by solid phase extraction was followed by separation on a CN column with a mobile phase of methanol–water (95:5, v/v) containing 0.2% formic acid. Mass spectrometric detection in the positive ion mode was carried out by selected reaction monitoring (SRM) of the transitions at m/z 403.0→372.0 for cyclovirobuxine D and m/z 325.0→234.0 for citalopram (internal standard). The method was linear in the range 10–200 ng/L with LLOQ of 10 ng/L, recovery >85%, and no significant matrix effects. Intra- and inter-day precisions were all <9% with accuracies of 94.0–104.8%. The method was successfully applied to a pharmacokinetic study involving a single oral administration of a 2 mg cyclovirobuxine D tablet to twenty-two healthy Chinese volunteers. |
| first_indexed | 2024-12-13T20:40:02Z |
| format | Article |
| id | doaj.art-46c830ead5b54cb1b2a430e62943c4c4 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-12-13T20:40:02Z |
| publishDate | 2011-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-46c830ead5b54cb1b2a430e62943c4c42022-12-21T23:32:10ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432011-10-011318418810.1016/j.apsb.2011.06.012Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic studyLing-li Mu0Peng Yu1Li-hua He2Medical College, Hunan Normal University, Changsha 410006, ChinaSchool of Pharmaceutical Sciences, Central South University, Changsha 410013, ChinaMedical College, Hunan Normal University, Changsha 410006, ChinaA sensitive and reliable method based on liquid chromatography tandem mass spectrometry (LC–MS/MS) for the quantitation of cyclovirobuxine D in human plasma has been developed and validated. Sample preparation by solid phase extraction was followed by separation on a CN column with a mobile phase of methanol–water (95:5, v/v) containing 0.2% formic acid. Mass spectrometric detection in the positive ion mode was carried out by selected reaction monitoring (SRM) of the transitions at m/z 403.0→372.0 for cyclovirobuxine D and m/z 325.0→234.0 for citalopram (internal standard). The method was linear in the range 10–200 ng/L with LLOQ of 10 ng/L, recovery >85%, and no significant matrix effects. Intra- and inter-day precisions were all <9% with accuracies of 94.0–104.8%. The method was successfully applied to a pharmacokinetic study involving a single oral administration of a 2 mg cyclovirobuxine D tablet to twenty-two healthy Chinese volunteers.http://www.sciencedirect.com/science/article/pii/S2211383511000633Cyclovirobuxine DLC–MS/MSHuman plasmaPharmacokinetics |
| spellingShingle | Ling-li Mu Peng Yu Li-hua He Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study Acta Pharmaceutica Sinica B Cyclovirobuxine D LC–MS/MS Human plasma Pharmacokinetics |
| title | Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| title_full | Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| title_fullStr | Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| title_full_unstemmed | Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| title_short | Determination of cyclovirobuxine D in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| title_sort | determination of cyclovirobuxine d in human plasma by liquid chromatography tandem mass spectrometry and application in a pharmacokinetic study |
| topic | Cyclovirobuxine D LC–MS/MS Human plasma Pharmacokinetics |
| url | http://www.sciencedirect.com/science/article/pii/S2211383511000633 |
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