Effect of carboxymethyl chito-oligosaccharide on cysteine absorption in intestinal porcine epithelial cells
Oxidative stress is associated with retarded growth and the initiation and progression of diseases in pigs. The carboxymethylation of chito-oligosaccharide (COS) can improve its antioxidant activity for such issues. Herein, an orthogonal experiment L9 (34) test design was used to optimise the prepar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2022-07-01
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| Series: | Czech Journal of Animal Science |
| Subjects: | |
| Online Access: | https://cjas.agriculturejournals.cz/artkey/cjs-202207-0002_effect-of-carboxymethyl-chito-oligosaccharide-on-cysteine-absorption-in-intestinal-porcine-epithelial-cells.php |
| Summary: | Oxidative stress is associated with retarded growth and the initiation and progression of diseases in pigs. The carboxymethylation of chito-oligosaccharide (COS) can improve its antioxidant activity for such issues. Herein, an orthogonal experiment L9 (34) test design was used to optimise the preparation conditions of carboxymethyl (CM)-COS. Fourier-transform infrared analysis evidenced the carboxymethylation of COS, and an in vitro study indicated that the hydroxyl radical scavenging ability of CM-COS is superior to that of COS. CM-COS can also better promote the absorption of cysteine and increase the expression of the amino acid transport system b0,+ in intestinal porcine epithelial cells. The results suggested that CM-COS can effectively resist oxidative damage by promoting cysteine absorption mediated by transport system b0,+, which provides important information regarding the antioxidative damage application of COS and CM-COS in the pig farming industry. |
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| ISSN: | 1212-1819 1805-9309 |