A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants
The emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1093080/full |
| _version_ | 1797956515117662208 |
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| author | Huifang Xu Tiantian Wang Peng Sun Xuchen Hou Xin Gong Bin Zhang Jun Wu Bo Liu |
| author_facet | Huifang Xu Tiantian Wang Peng Sun Xuchen Hou Xin Gong Bin Zhang Jun Wu Bo Liu |
| author_sort | Huifang Xu |
| collection | DOAJ |
| description | The emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the pandemic. In this study, the receptor binding domain (RBD) of the SARS-CoV-2 Beta variant was successfully expressed through a glycoengineered yeast platform. To pursue a more broad-spectrum vaccination strategy, RBD-Beta and RBD-wild type were mixed at the ratio of 1:1 with Al(OH)3 and CpG double adjuvants for the immunization of BALB/c mice. This bivalent vaccine stimulated robust conjugated antibody titers and a broader spectrum of neutralizing antibody titers. These results suggested that a bivalent vaccine of RBD-Beta and RBD-wild type could be a possible broad-spectrum vaccination strategy. |
| first_indexed | 2024-04-10T23:50:04Z |
| format | Article |
| id | doaj.art-46ca1d0407b04a2b8a546b1a2832dc71 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T23:50:04Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-46ca1d0407b04a2b8a546b1a2832dc712023-01-10T19:16:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10930801093080A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variantsHuifang XuTiantian WangPeng SunXuchen HouXin GongBin ZhangJun WuBo LiuThe emergence of severe acute respiratory syndrome coronavirus type II (SARS-CoV-2) variants have led to a decline in the protection of existing vaccines and antibodies, and there is an urgent need for a broad-spectrum vaccination strategy to reduce the pressure on the prevention and control of the pandemic. In this study, the receptor binding domain (RBD) of the SARS-CoV-2 Beta variant was successfully expressed through a glycoengineered yeast platform. To pursue a more broad-spectrum vaccination strategy, RBD-Beta and RBD-wild type were mixed at the ratio of 1:1 with Al(OH)3 and CpG double adjuvants for the immunization of BALB/c mice. This bivalent vaccine stimulated robust conjugated antibody titers and a broader spectrum of neutralizing antibody titers. These results suggested that a bivalent vaccine of RBD-Beta and RBD-wild type could be a possible broad-spectrum vaccination strategy.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1093080/fullSARS-CoV-2RBDsubunit vaccinebivalent vaccineglycoengineered yeast |
| spellingShingle | Huifang Xu Tiantian Wang Peng Sun Xuchen Hou Xin Gong Bin Zhang Jun Wu Bo Liu A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants Frontiers in Microbiology SARS-CoV-2 RBD subunit vaccine bivalent vaccine glycoengineered yeast |
| title | A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants |
| title_full | A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants |
| title_fullStr | A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants |
| title_full_unstemmed | A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants |
| title_short | A bivalent subunit vaccine efficiently produced in Pichia pastoris against SARS-CoV-2 and emerging variants |
| title_sort | bivalent subunit vaccine efficiently produced in pichia pastoris against sars cov 2 and emerging variants |
| topic | SARS-CoV-2 RBD subunit vaccine bivalent vaccine glycoengineered yeast |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1093080/full |
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