Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

Abstract Background De novo DNA methylation triggered by short interfering RNAs is called RNA-directed DNA methylation (RdDM). Transcriptional gene silencing (TGS) through RdDM can be induced using a viral vector. We have previously induced RdDM on the 35S promoter in the green fluorescent protein (...

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Bibliographic Details
Main Authors: Wataru Matsunaga, Hanako Shimura, Senri Shirakawa, Reika Isoda, Tsuyoshi Inukai, Takeshi Matsumura, Chikara Masuta
Format: Article
Language:English
Published: BMC 2019-01-01
Series:BMC Plant Biology
Subjects:
CaMV 35S promoter
Nicotiana benthamiana
RNA-directed DNA methylation
VITGS
Online Access:http://link.springer.com/article/10.1186/s12870-019-1628-y

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