Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament
In this work, we developed an alternative manufacturing paper-based microfluidics method through 3D printing and wax filament. Microfluidic paper-based analytical devices (µPADs) are low-cost and easy-to-manufacture tools used for various chemical and biological analyses and studies. Paper-based mic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Talanta Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831922000595 |
| _version_ | 1811293941348171776 |
|---|---|
| author | Antonio Espinosa Joannes Diaz Edgar Vazquez Lina Acosta Arianna Santiago Lisandro Cunci |
| author_facet | Antonio Espinosa Joannes Diaz Edgar Vazquez Lina Acosta Arianna Santiago Lisandro Cunci |
| author_sort | Antonio Espinosa |
| collection | DOAJ |
| description | In this work, we developed an alternative manufacturing paper-based microfluidics method through 3D printing and wax filament. Microfluidic paper-based analytical devices (µPADs) are low-cost and easy-to-manufacture tools used for various chemical and biological analyses and studies. Paper-based microfluidics with wax has been limited as the manufacturers have discontinued most wax printing equipment. We aim to develop a low-cost and accessible manufacturing method that can replace conventional wax-on paper-based microfluidic manufacturing methods. Using highly available commercial 3D printing technology and wax filament, we could create hydrophobic wax barriers on the surface of different paper types. The properties and limits of this manufacturing method were characterized. Moreover, using this paper-based microfluidic manufacturing method, we were able to measure dopamine electrochemically using µPAD as a passive flow-based method in concentrations as low as 1 nM using injections as small as 15 µL. |
| first_indexed | 2024-04-13T05:08:56Z |
| format | Article |
| id | doaj.art-46cc88d618fd4052b901fbf35c8ce40f |
| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-04-13T05:08:56Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj.art-46cc88d618fd4052b901fbf35c8ce40f2022-12-22T03:01:05ZengElsevierTalanta Open2666-83192022-12-016100142Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filamentAntonio Espinosa0Joannes Diaz1Edgar Vazquez2Lina Acosta3Arianna Santiago4Lisandro Cunci5Department of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesDepartment of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesDepartment of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesDepartment of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesDepartment of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesCorresponding author.; Department of Chemistry, Universidad Ana G. Méndez – Recinto de Gurabo, Carr. 189, Km 3.3, Gurabo, Puerto Rico 00778, United StatesIn this work, we developed an alternative manufacturing paper-based microfluidics method through 3D printing and wax filament. Microfluidic paper-based analytical devices (µPADs) are low-cost and easy-to-manufacture tools used for various chemical and biological analyses and studies. Paper-based microfluidics with wax has been limited as the manufacturers have discontinued most wax printing equipment. We aim to develop a low-cost and accessible manufacturing method that can replace conventional wax-on paper-based microfluidic manufacturing methods. Using highly available commercial 3D printing technology and wax filament, we could create hydrophobic wax barriers on the surface of different paper types. The properties and limits of this manufacturing method were characterized. Moreover, using this paper-based microfluidic manufacturing method, we were able to measure dopamine electrochemically using µPAD as a passive flow-based method in concentrations as low as 1 nM using injections as small as 15 µL.http://www.sciencedirect.com/science/article/pii/S2666831922000595Microfluidic paper-based analytical devicesWax filament3D printerPassive flow |
| spellingShingle | Antonio Espinosa Joannes Diaz Edgar Vazquez Lina Acosta Arianna Santiago Lisandro Cunci Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament Talanta Open Microfluidic paper-based analytical devices Wax filament 3D printer Passive flow |
| title | Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament |
| title_full | Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament |
| title_fullStr | Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament |
| title_full_unstemmed | Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament |
| title_short | Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament |
| title_sort | fabrication of paper based microfluidic devices using a 3d printer and a commercially available wax filament |
| topic | Microfluidic paper-based analytical devices Wax filament 3D printer Passive flow |
| url | http://www.sciencedirect.com/science/article/pii/S2666831922000595 |
| work_keys_str_mv | AT antonioespinosa fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament AT joannesdiaz fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament AT edgarvazquez fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament AT linaacosta fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament AT ariannasantiago fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament AT lisandrocunci fabricationofpaperbasedmicrofluidicdevicesusinga3dprinterandacommerciallyavailablewaxfilament |