Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice
Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of d...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-12-01
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| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221367111500315X |
| _version_ | 1828812058589659136 |
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| author | Bryan Holvoet Mattia Quattrocelli Sarah Belderbos Lore Pollaris Esther Wolfs Olivier Gheysens Rik Gijsbers Jeroen Vanoirbeek Catherine M. Verfaillie Maurilio Sampaolesi Christophe M. Deroose |
| author_facet | Bryan Holvoet Mattia Quattrocelli Sarah Belderbos Lore Pollaris Esther Wolfs Olivier Gheysens Rik Gijsbers Jeroen Vanoirbeek Catherine M. Verfaillie Maurilio Sampaolesi Christophe M. Deroose |
| author_sort | Bryan Holvoet |
| collection | DOAJ |
| description | Muscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells. |
| first_indexed | 2024-12-12T09:36:45Z |
| format | Article |
| id | doaj.art-46cc9c97003946c2b4fbf974036f46dd |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-12T09:36:45Z |
| publishDate | 2015-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-46cc9c97003946c2b4fbf974036f46dd2022-12-22T00:28:41ZengElsevierStem Cell Reports2213-67112015-12-01561183119510.1016/j.stemcr.2015.10.018Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic MiceBryan Holvoet0Mattia Quattrocelli1Sarah Belderbos2Lore Pollaris3Esther Wolfs4Olivier Gheysens5Rik Gijsbers6Jeroen Vanoirbeek7Catherine M. Verfaillie8Maurilio Sampaolesi9Christophe M. Deroose10Department of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, BelgiumDepartment of Development and Regeneration, Translational Cardiomyology Lab, KU Leuven, Leuven 3000, BelgiumDepartment of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, BelgiumDepartment of Public Health and Primary Care, Centre for Environment and Health, KU Leuven, Leuven 3000, BelgiumDepartment of Morphology, Biomedical Research Institute, Lab of Histology, Universiteit Hasselt, Diepenbeek 3590, BelgiumDepartment of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, BelgiumDepartment of Pharmaceutical and Pharmacological Sciences, Laboratory of Molecular Virology and Gene Therapy, Leuven Viral Vector Core, KU Leuven, Leuven 3000, BelgiumDepartment of Public Health and Primary Care, Centre for Environment and Health, KU Leuven, Leuven 3000, BelgiumDepartment of Development and Regeneration, Stem Cell Institute Leuven, KU Leuven, Leuven 3000, BelgiumDepartment of Development and Regeneration, Translational Cardiomyology Lab, KU Leuven, Leuven 3000, BelgiumDepartment of Imaging and Pathology, Nuclear Medicine and Molecular Imaging, KU Leuven, Leuven 3000, BelgiumMuscular dystrophies are a heterogeneous group of myopathies, characterized by muscle weakness and degeneration, without curative treatment. Mesoangioblasts (MABs) have been proposed as a potential regenerative therapy. To improve our understanding of the in vivo behavior of MABs and the effect of different immunosuppressive therapies, like cyclosporine A or co-stimulation-adhesion blockade therapy, on cell survival noninvasive cell monitoring is required. Therefore, cells were transduced with a lentiviral vector encoding firefly luciferase (Fluc) and the human sodium iodide transporter (hNIS) to allow cell monitoring via bioluminescence imaging (BLI) and small-animal positron emission tomography (PET). Non-H2 matched mMABs were injected in the femoral artery of dystrophic mice and were clearly visible via small-animal PET and BLI. Based on noninvasive imaging data, we were able to show that co-stim was clearly superior to CsA in reducing cell rejection and this was mediated via a reduction in cytotoxic T cells and upregulation of regulatory T cells.http://www.sciencedirect.com/science/article/pii/S221367111500315X |
| spellingShingle | Bryan Holvoet Mattia Quattrocelli Sarah Belderbos Lore Pollaris Esther Wolfs Olivier Gheysens Rik Gijsbers Jeroen Vanoirbeek Catherine M. Verfaillie Maurilio Sampaolesi Christophe M. Deroose Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice Stem Cell Reports |
| title | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice |
| title_full | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice |
| title_fullStr | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice |
| title_full_unstemmed | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice |
| title_short | Sodium Iodide Symporter PET and BLI Noninvasively Reveal Mesoangioblast Survival in Dystrophic Mice |
| title_sort | sodium iodide symporter pet and bli noninvasively reveal mesoangioblast survival in dystrophic mice |
| url | http://www.sciencedirect.com/science/article/pii/S221367111500315X |
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