Application of green fluorescent protein for monitoring phenol-degrading strains
<p class="Default" style="text-align: justify;"><span lang="EN-US">Several methods have been developed for detecting microorganisms in environmental samples. Some systems for incorporating reporter genes, such as <em>lux </em>or the green fluores...
| Main Authors: | , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2011-12-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/30078 |
| _version_ | 1818322610925076480 |
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| author | Ana Milena Valderrama F. Julia Raquel Acero R. |
| author_facet | Ana Milena Valderrama F. Julia Raquel Acero R. |
| author_sort | Ana Milena Valderrama F. |
| collection | DOAJ |
| description | <p class="Default" style="text-align: justify;"><span lang="EN-US">Several methods have been developed for detecting microorganisms in environmental samples. Some systems for incorporating reporter genes, such as <em>lux </em>or the green fluorescent protein (GFP) gene, have been developed recently This study describes <em>gfp </em>gene marking of a phenol degrading strain, its evaluation and monitoring in a bioreactor containing refinery sour water. Tagged strains were obtained having the same physiological and metabolic characteristics as the parent strain. Fluorescent expression was kept stable with no selection for more than 50 consecutive generations and tagged strains were recovered from the bioreactor after forty-five days of phenol-degradation treatment. </span></p> |
| first_indexed | 2024-12-13T10:59:32Z |
| format | Article |
| id | doaj.art-46d45370ac464d3cb32bd6e7c3902528 |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-13T10:59:32Z |
| publishDate | 2011-12-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-46d45370ac464d3cb32bd6e7c39025282022-12-21T23:49:18ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582011-12-0132788426556Application of green fluorescent protein for monitoring phenol-degrading strainsAna Milena Valderrama F.0Julia Raquel Acero R.1Instituto Colombiano del Petróleo, EcopetrolInstituto Colombiano del Petróleo, Ecopetrol<p class="Default" style="text-align: justify;"><span lang="EN-US">Several methods have been developed for detecting microorganisms in environmental samples. Some systems for incorporating reporter genes, such as <em>lux </em>or the green fluorescent protein (GFP) gene, have been developed recently This study describes <em>gfp </em>gene marking of a phenol degrading strain, its evaluation and monitoring in a bioreactor containing refinery sour water. Tagged strains were obtained having the same physiological and metabolic characteristics as the parent strain. Fluorescent expression was kept stable with no selection for more than 50 consecutive generations and tagged strains were recovered from the bioreactor after forty-five days of phenol-degradation treatment. </span></p>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/30078GFPaguas residuales industrialesbiorremediaciónbiosensor |
| spellingShingle | Ana Milena Valderrama F. Julia Raquel Acero R. Application of green fluorescent protein for monitoring phenol-degrading strains Revista Colombiana de Biotecnología GFP aguas residuales industriales biorremediación biosensor |
| title | Application of green fluorescent protein for monitoring phenol-degrading strains |
| title_full | Application of green fluorescent protein for monitoring phenol-degrading strains |
| title_fullStr | Application of green fluorescent protein for monitoring phenol-degrading strains |
| title_full_unstemmed | Application of green fluorescent protein for monitoring phenol-degrading strains |
| title_short | Application of green fluorescent protein for monitoring phenol-degrading strains |
| title_sort | application of green fluorescent protein for monitoring phenol degrading strains |
| topic | GFP aguas residuales industriales biorremediación biosensor |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/30078 |
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