Identification of novel regulators in T-cell differentiation of aplastic anemia patients
<p>Abstract</p> <p>Background</p> <p>Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular imm...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-10-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/7/263 |
| _version_ | 1811249981149937664 |
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| author | Probst-Kepper Michael Grosse Jens Ivanyi Philipp Piao Wenji Pförtner Susanne Hunger J Katrin Geffers Robert Franzke Anke Ganser Arnold Buer Jan |
| author_facet | Probst-Kepper Michael Grosse Jens Ivanyi Philipp Piao Wenji Pförtner Susanne Hunger J Katrin Geffers Robert Franzke Anke Ganser Arnold Buer Jan |
| author_sort | Probst-Kepper Michael |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular immune response. To overcome this technical problem we performed a microarray analysis of CD3<sup>+ </sup>T-cells derived from bone marrow aspirates and peripheral blood samples of newly diagnosed AA patients and healthy volunteers. Two AA patients were additionally analyzed after achieving a partial remission following immunosuppression. The regulation of selected candidate genes was confirmed by real-time RT-PCR.</p> <p>Results</p> <p>Among more than 22.200 transcripts, 583 genes were differentially expressed in the bone marrow of AA patients compared to healthy controls. Dysregulated genes are involved in T-cell mediated cytotoxicity, immune response of Th1 differentiated T-cells, and major regulators of immune function. In hematological remission the expression levels of several candidate genes tend to normalize, such as immune regulators and genes involved in proinflammatory immune response.</p> <p>Conclusion</p> <p>Our study suggests a pivotal role of Th1/Tc1 differentiated T-cells in immune-mediated marrow destruction of AA patients. Most importantly, immune regulatory genes could be identified, which are likely involved in the recovery of hematopoiesis and may help to design new therapeutic strategies in bone marrow failure syndromes.</p> |
| first_indexed | 2024-04-12T15:57:01Z |
| format | Article |
| id | doaj.art-46d5f0500cf5486db6faa8cc0acf7970 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T15:57:01Z |
| publishDate | 2006-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-46d5f0500cf5486db6faa8cc0acf79702022-12-22T03:26:20ZengBMCBMC Genomics1471-21642006-10-017126310.1186/1471-2164-7-263Identification of novel regulators in T-cell differentiation of aplastic anemia patientsProbst-Kepper MichaelGrosse JensIvanyi PhilippPiao WenjiPförtner SusanneHunger J KatrinGeffers RobertFranzke AnkeGanser ArnoldBuer Jan<p>Abstract</p> <p>Background</p> <p>Aplastic anemia (AA) is a bone marrow failure syndrome mostly characterized by an immune-mediated destruction of marrow hematopoietic progenitor/stem cells. The resulting hypocellularity limits a detailed analysis of the cellular immune response. To overcome this technical problem we performed a microarray analysis of CD3<sup>+ </sup>T-cells derived from bone marrow aspirates and peripheral blood samples of newly diagnosed AA patients and healthy volunteers. Two AA patients were additionally analyzed after achieving a partial remission following immunosuppression. The regulation of selected candidate genes was confirmed by real-time RT-PCR.</p> <p>Results</p> <p>Among more than 22.200 transcripts, 583 genes were differentially expressed in the bone marrow of AA patients compared to healthy controls. Dysregulated genes are involved in T-cell mediated cytotoxicity, immune response of Th1 differentiated T-cells, and major regulators of immune function. In hematological remission the expression levels of several candidate genes tend to normalize, such as immune regulators and genes involved in proinflammatory immune response.</p> <p>Conclusion</p> <p>Our study suggests a pivotal role of Th1/Tc1 differentiated T-cells in immune-mediated marrow destruction of AA patients. Most importantly, immune regulatory genes could be identified, which are likely involved in the recovery of hematopoiesis and may help to design new therapeutic strategies in bone marrow failure syndromes.</p>http://www.biomedcentral.com/1471-2164/7/263 |
| spellingShingle | Probst-Kepper Michael Grosse Jens Ivanyi Philipp Piao Wenji Pförtner Susanne Hunger J Katrin Geffers Robert Franzke Anke Ganser Arnold Buer Jan Identification of novel regulators in T-cell differentiation of aplastic anemia patients BMC Genomics |
| title | Identification of novel regulators in T-cell differentiation of aplastic anemia patients |
| title_full | Identification of novel regulators in T-cell differentiation of aplastic anemia patients |
| title_fullStr | Identification of novel regulators in T-cell differentiation of aplastic anemia patients |
| title_full_unstemmed | Identification of novel regulators in T-cell differentiation of aplastic anemia patients |
| title_short | Identification of novel regulators in T-cell differentiation of aplastic anemia patients |
| title_sort | identification of novel regulators in t cell differentiation of aplastic anemia patients |
| url | http://www.biomedcentral.com/1471-2164/7/263 |
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