Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis
Ionizing radiation has become an inevitable health concern emanating from natural sources like space travel and from artificial sources like medical therapies. In general, exposure to ionizing radiation such as γ-rays is one of the methods currently used to stress specific model systems. In this stu...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2012-08-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1420-3049/17/8/9462 |
| _version_ | 1819007577224118272 |
|---|---|
| author | Jin Kyu Kim Changhyun Roh Insub Kim Uhee Jung Min-Kyoung Park Seolhwa Kim |
| author_facet | Jin Kyu Kim Changhyun Roh Insub Kim Uhee Jung Min-Kyoung Park Seolhwa Kim |
| author_sort | Jin Kyu Kim |
| collection | DOAJ |
| description | Ionizing radiation has become an inevitable health concern emanating from natural sources like space travel and from artificial sources like medical therapies. In general, exposure to ionizing radiation such as γ-rays is one of the methods currently used to stress specific model systems. In this study, we elucidated the long-term effect of acute and fractionated irradiation on DCX-positive cells in hippocampal neurogenesis. Groups of two-month-old C57BL/6 female mice were exposed to whole-body irradiation at acute dose (5 Gy) or fractional doses (1 Gy × 5 times and 0.5 Gy × 10 times). Six months after exposure to γ-irradiation, the hippocampus was analyzed. Doublecortin (DCX) immunohistochemistry was used to measure changes of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). The number of DCX-positive cells was significantly decreased in all acute and fractionally irradiation groups. The long-term changes in DCX-positive cells triggered by radiation exposure showed a very different pattern to the short-term changes which tended to return to the control level in previous studies. Furthermore, the number of DCX-positive cells was relatively lower in the acute irradiation group than the fractional irradiation groups (approximately 3.6-fold), suggesting the biological change on hippocampal neurogenesis was more susceptible to being damaged by acute than fractional irradiation. These results suggest that the exposure to γ-irradiation as a long-term effect can trigger biological responses resulting in the inhibition of hippocampal neurogenesis. |
| first_indexed | 2024-12-21T00:26:47Z |
| format | Article |
| id | doaj.art-46d7a8d1bbc54d4593885278f0e4051c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-21T00:26:47Z |
| publishDate | 2012-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-46d7a8d1bbc54d4593885278f0e4051c2022-12-21T19:21:59ZengMDPI AGMolecules1420-30492012-08-011789462946810.3390/molecules17089462Effect of Acute and Fractionated Irradiation on Hippocampal NeurogenesisJin Kyu KimChanghyun RohInsub KimUhee JungMin-Kyoung ParkSeolhwa KimIonizing radiation has become an inevitable health concern emanating from natural sources like space travel and from artificial sources like medical therapies. In general, exposure to ionizing radiation such as γ-rays is one of the methods currently used to stress specific model systems. In this study, we elucidated the long-term effect of acute and fractionated irradiation on DCX-positive cells in hippocampal neurogenesis. Groups of two-month-old C57BL/6 female mice were exposed to whole-body irradiation at acute dose (5 Gy) or fractional doses (1 Gy × 5 times and 0.5 Gy × 10 times). Six months after exposure to γ-irradiation, the hippocampus was analyzed. Doublecortin (DCX) immunohistochemistry was used to measure changes of neurogenesis in the subgranular zone (SGZ) of the hippocampal dentate gyrus (DG). The number of DCX-positive cells was significantly decreased in all acute and fractionally irradiation groups. The long-term changes in DCX-positive cells triggered by radiation exposure showed a very different pattern to the short-term changes which tended to return to the control level in previous studies. Furthermore, the number of DCX-positive cells was relatively lower in the acute irradiation group than the fractional irradiation groups (approximately 3.6-fold), suggesting the biological change on hippocampal neurogenesis was more susceptible to being damaged by acute than fractional irradiation. These results suggest that the exposure to γ-irradiation as a long-term effect can trigger biological responses resulting in the inhibition of hippocampal neurogenesis.http://www.mdpi.com/1420-3049/17/8/9462neurogenesisionizing radiationimmunohistochemistrydoublecortinlong-term effects |
| spellingShingle | Jin Kyu Kim Changhyun Roh Insub Kim Uhee Jung Min-Kyoung Park Seolhwa Kim Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis Molecules neurogenesis ionizing radiation immunohistochemistry doublecortin long-term effects |
| title | Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis |
| title_full | Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis |
| title_fullStr | Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis |
| title_full_unstemmed | Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis |
| title_short | Effect of Acute and Fractionated Irradiation on Hippocampal Neurogenesis |
| title_sort | effect of acute and fractionated irradiation on hippocampal neurogenesis |
| topic | neurogenesis ionizing radiation immunohistochemistry doublecortin long-term effects |
| url | http://www.mdpi.com/1420-3049/17/8/9462 |
| work_keys_str_mv | AT jinkyukim effectofacuteandfractionatedirradiationonhippocampalneurogenesis AT changhyunroh effectofacuteandfractionatedirradiationonhippocampalneurogenesis AT insubkim effectofacuteandfractionatedirradiationonhippocampalneurogenesis AT uheejung effectofacuteandfractionatedirradiationonhippocampalneurogenesis AT minkyoungpark effectofacuteandfractionatedirradiationonhippocampalneurogenesis AT seolhwakim effectofacuteandfractionatedirradiationonhippocampalneurogenesis |