Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus)
This work provides information on the blue fox ejaculated sperm quality needed for seminal dose calculations. Twenty semen samples, obtained by masturbation, were analyzed for kinematic and morphometric parameters by using CASA-Mot and CASA-Morph system and principal component (PC) analysis. For mot...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=2;spage=154;epage=159;aulast=Soler |
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| author | Carles Soler Jesús Contell Lorena Bori María Sancho Almudena García-Molina Anthony Valverde Jan Segarvall |
| author_facet | Carles Soler Jesús Contell Lorena Bori María Sancho Almudena García-Molina Anthony Valverde Jan Segarvall |
| author_sort | Carles Soler |
| collection | DOAJ |
| description | This work provides information on the blue fox ejaculated sperm quality needed for seminal dose calculations. Twenty semen samples, obtained by masturbation, were analyzed for kinematic and morphometric parameters by using CASA-Mot and CASA-Morph system and principal component (PC) analysis. For motility, eight kinematic parameters were evaluated, which were reduced to PC1, related to linear variables, and PC2, related to oscillatory movement. The whole population was divided into three independent subpopulations: SP1, fast cells with linear movement; SP2, slow cells and nonoscillatory motility; and SP3, medium speed cells and oscillatory movement. In almost all cases, the subpopulation distribution by animal was significantly different. Head morphology analysis generated four size and four shape parameters, which were reduced to PC1, related to size, and PC2, related to shape of the cells. Three morphometric subpopulations existed: SP1: large oval cells; SP2: medium size elongated cells; and SP3: small and short cells. The subpopulation distribution differed between animals. Combining the kinematic and morphometric datasets produced PC1, related to morphometric parameters, and PC2, related to kinematics, which generated four sperm subpopulations - SP1: high oscillatory motility, large and short heads; SP2: medium velocity with small and short heads; SP3: slow motion small and elongated cells; and SP4: high linear speed and large elongated cells. Subpopulation distribution was different in all animals. The establishment of sperm subpopulations from kinematic, morphometric, and combined variables not only improves the well-defined fox semen characteristics and offers a good conceptual basis for fertility and sperm preservation techniques in this species, but also opens the door to use this approach in other species, included humans. |
| first_indexed | 2024-12-12T23:15:49Z |
| format | Article |
| id | doaj.art-46e105c8a72945188abd09aecac81f0f |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-12-12T23:15:49Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-46e105c8a72945188abd09aecac81f0f2022-12-22T00:08:28ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622017-01-0119215415910.4103/1008-682X.188445Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus)Carles SolerJesús ContellLorena BoriMaría SanchoAlmudena García-MolinaAnthony ValverdeJan SegarvallThis work provides information on the blue fox ejaculated sperm quality needed for seminal dose calculations. Twenty semen samples, obtained by masturbation, were analyzed for kinematic and morphometric parameters by using CASA-Mot and CASA-Morph system and principal component (PC) analysis. For motility, eight kinematic parameters were evaluated, which were reduced to PC1, related to linear variables, and PC2, related to oscillatory movement. The whole population was divided into three independent subpopulations: SP1, fast cells with linear movement; SP2, slow cells and nonoscillatory motility; and SP3, medium speed cells and oscillatory movement. In almost all cases, the subpopulation distribution by animal was significantly different. Head morphology analysis generated four size and four shape parameters, which were reduced to PC1, related to size, and PC2, related to shape of the cells. Three morphometric subpopulations existed: SP1: large oval cells; SP2: medium size elongated cells; and SP3: small and short cells. The subpopulation distribution differed between animals. Combining the kinematic and morphometric datasets produced PC1, related to morphometric parameters, and PC2, related to kinematics, which generated four sperm subpopulations - SP1: high oscillatory motility, large and short heads; SP2: medium velocity with small and short heads; SP3: slow motion small and elongated cells; and SP4: high linear speed and large elongated cells. Subpopulation distribution was different in all animals. The establishment of sperm subpopulations from kinematic, morphometric, and combined variables not only improves the well-defined fox semen characteristics and offers a good conceptual basis for fertility and sperm preservation techniques in this species, but also opens the door to use this approach in other species, included humans.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=2;spage=154;epage=159;aulast=Solerintegration of motility and morphology; principal component analysis; sperm morphometry; subpopulation |
| spellingShingle | Carles Soler Jesús Contell Lorena Bori María Sancho Almudena García-Molina Anthony Valverde Jan Segarvall Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) Asian Journal of Andrology integration of motility and morphology; principal component analysis; sperm morphometry; subpopulation |
| title | Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) |
| title_full | Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) |
| title_fullStr | Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) |
| title_full_unstemmed | Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) |
| title_short | Sperm kinematic, head morphometric and kinetic-morphometric subpopulations in the blue fox (Alopex lagopus) |
| title_sort | sperm kinematic head morphometric and kinetic morphometric subpopulations in the blue fox alopex lagopus |
| topic | integration of motility and morphology; principal component analysis; sperm morphometry; subpopulation |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2017;volume=19;issue=2;spage=154;epage=159;aulast=Soler |
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