Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved]
Dendritic spines and synapses are critical for neuronal communication, and they are perturbed in many neurological disorders; however, the study of these structures in living cells has been hindered by their small size. Super resolution microscopy, unlike conventional light microscopy, is diffractio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
F1000 Research Ltd
2016-06-01
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| Series: | F1000Research |
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| Online Access: | http://f1000research.com/articles/5-1468/v1 |
| _version_ | 1818119476323811328 |
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| author | Cristina M. Robinson Mikin R. Patel Donna J. Webb |
| author_facet | Cristina M. Robinson Mikin R. Patel Donna J. Webb |
| author_sort | Cristina M. Robinson |
| collection | DOAJ |
| description | Dendritic spines and synapses are critical for neuronal communication, and they are perturbed in many neurological disorders; however, the study of these structures in living cells has been hindered by their small size. Super resolution microscopy, unlike conventional light microscopy, is diffraction unlimited and thus is well suited for imaging small structures, such as dendritic spines and synapses. Super resolution microscopy has already revealed important new information about spine and synapse morphology, actin remodeling, and nanodomain composition in both healthy cells and diseased states. In this review, we highlight the advancements in probes that make super resolution more amenable to live-cell imaging of spines and synapses. We also discuss recent data obtained by super resolution microscopy that has advanced our knowledge of dendritic spine and synapse structure, organization, and dynamics in both healthy and diseased contexts. Finally, we propose a series of critical questions for understanding spine and synapse formation and maturation that super resolution microscopy is poised to answer. |
| first_indexed | 2024-12-11T05:10:48Z |
| format | Article |
| id | doaj.art-46e3ad9fb2b7429986dfce6482e7b43b |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-11T05:10:48Z |
| publishDate | 2016-06-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-46e3ad9fb2b7429986dfce6482e7b43b2022-12-22T01:19:55ZengF1000 Research LtdF1000Research2046-14022016-06-01510.12688/f1000research.8649.19307Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved]Cristina M. Robinson0Mikin R. Patel1Donna J. Webb2Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USADepartment of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USADepartment of Cancer Biology, Vanderbilt University, Nashville, Tennessee, USADendritic spines and synapses are critical for neuronal communication, and they are perturbed in many neurological disorders; however, the study of these structures in living cells has been hindered by their small size. Super resolution microscopy, unlike conventional light microscopy, is diffraction unlimited and thus is well suited for imaging small structures, such as dendritic spines and synapses. Super resolution microscopy has already revealed important new information about spine and synapse morphology, actin remodeling, and nanodomain composition in both healthy cells and diseased states. In this review, we highlight the advancements in probes that make super resolution more amenable to live-cell imaging of spines and synapses. We also discuss recent data obtained by super resolution microscopy that has advanced our knowledge of dendritic spine and synapse structure, organization, and dynamics in both healthy and diseased contexts. Finally, we propose a series of critical questions for understanding spine and synapse formation and maturation that super resolution microscopy is poised to answer.http://f1000research.com/articles/5-1468/v1CytoskeletonExperimental Biophysical MethodsMacromolecular Assemblies & MachinesNeurobiology of Disease & RegenerationNeuronal & Glial Cell BiologyNeuronal Signaling Mechanisms |
| spellingShingle | Cristina M. Robinson Mikin R. Patel Donna J. Webb Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] F1000Research Cytoskeleton Experimental Biophysical Methods Macromolecular Assemblies & Machines Neurobiology of Disease & Regeneration Neuronal & Glial Cell Biology Neuronal Signaling Mechanisms |
| title | Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] |
| title_full | Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] |
| title_fullStr | Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] |
| title_full_unstemmed | Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] |
| title_short | Super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines [version 1; referees: 2 approved] |
| title_sort | super resolution microscopy is poised to reveal new insights into the formation and maturation of dendritic spines version 1 referees 2 approved |
| topic | Cytoskeleton Experimental Biophysical Methods Macromolecular Assemblies & Machines Neurobiology of Disease & Regeneration Neuronal & Glial Cell Biology Neuronal Signaling Mechanisms |
| url | http://f1000research.com/articles/5-1468/v1 |
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