Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis
Background: Recombinant K39 antigen test is one of the recent experiences in rapid diagnosis of Visceral Leishmaniasis ,it consist of 39 amino acid which represent the main component of the protein of the Leishmania parasite and that the reaction occurs between the antibodies in the serum and ant...
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| Format: | Article |
| Language: | English |
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College of medicine/ University of Diyala
2019-05-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/238 |
| _version_ | 1797586304342425600 |
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| author | Ismail Ibrahim Latif Ashraf Jamail Mahmoud Yaseen Majed Salman |
| author_facet | Ismail Ibrahim Latif Ashraf Jamail Mahmoud Yaseen Majed Salman |
| author_sort | Ismail Ibrahim Latif |
| collection | DOAJ |
| description |
Background: Recombinant K39 antigen test is one of the recent experiences in rapid diagnosis of Visceral Leishmaniasis ,it consist of 39 amino acid which represent the main component of the protein of the Leishmania parasite and that the reaction occurs between the antibodies in the serum and antigens blotted in the rK39 strip test.
Objective: Find out possible reaction of rK39 antigen test on other diseases than visceral leishmaniasis, as well as to compare the test results on both serum and urine of same patients.
Patients and Methods: A total of 65 blood and urine samples were included in the study; 25 patients with cutaneous leishmaniasis attended Baquba Teaching Hospital, and 40 apparently healthy controls. All serum and urine samples were tested with rK39 strip test. 15 serum samples were randomly selected and Indirect Fluorescent Antibody Techniques for diagnosis of Leishmaniasis for the purpose of comparison of rK39 strip test.
Results: There was highly significant differences when using the rK39 strip test in urine and serum in patients infected with cutaneous leishmaniasis (P <0.05), and no significant difference between the sexes, as well as age group , as well as the absence of a significant difference between the rK39 strip test and Indirect Fluorescent Antibody Techniques.
Conclusion: rK39 strip antigen test gives positive results, may be due to greater phylogenetic proximity between Leishmania species, or due to mixed infection with Visceral Leishmaniasis.
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| first_indexed | 2024-03-11T00:21:25Z |
| format | Article |
| id | doaj.art-46e3dacfb6a64cb6b1cf322ce2915c84 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-11T00:21:25Z |
| publishDate | 2019-05-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-46e3dacfb6a64cb6b1cf322ce2915c842023-11-18T23:17:43ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822019-05-01102Performance of Recombinant rK39 antigen in the diagnosis of LeishmaniasisIsmail Ibrahim LatifAshraf Jamail MahmoudYaseen Majed Salman Background: Recombinant K39 antigen test is one of the recent experiences in rapid diagnosis of Visceral Leishmaniasis ,it consist of 39 amino acid which represent the main component of the protein of the Leishmania parasite and that the reaction occurs between the antibodies in the serum and antigens blotted in the rK39 strip test. Objective: Find out possible reaction of rK39 antigen test on other diseases than visceral leishmaniasis, as well as to compare the test results on both serum and urine of same patients. Patients and Methods: A total of 65 blood and urine samples were included in the study; 25 patients with cutaneous leishmaniasis attended Baquba Teaching Hospital, and 40 apparently healthy controls. All serum and urine samples were tested with rK39 strip test. 15 serum samples were randomly selected and Indirect Fluorescent Antibody Techniques for diagnosis of Leishmaniasis for the purpose of comparison of rK39 strip test. Results: There was highly significant differences when using the rK39 strip test in urine and serum in patients infected with cutaneous leishmaniasis (P <0.05), and no significant difference between the sexes, as well as age group , as well as the absence of a significant difference between the rK39 strip test and Indirect Fluorescent Antibody Techniques. Conclusion: rK39 strip antigen test gives positive results, may be due to greater phylogenetic proximity between Leishmania species, or due to mixed infection with Visceral Leishmaniasis. https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/238rK39 antigen test, leishmaniasis, IFAT |
| spellingShingle | Ismail Ibrahim Latif Ashraf Jamail Mahmoud Yaseen Majed Salman Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis Diyala Journal of Medicine rK39 antigen test, leishmaniasis, IFAT |
| title | Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis |
| title_full | Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis |
| title_fullStr | Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis |
| title_full_unstemmed | Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis |
| title_short | Performance of Recombinant rK39 antigen in the diagnosis of Leishmaniasis |
| title_sort | performance of recombinant rk39 antigen in the diagnosis of leishmaniasis |
| topic | rK39 antigen test, leishmaniasis, IFAT |
| url | https://www.djm.uodiyala.edu.iq/index.php/djm/article/view/238 |
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