Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, RCaMPs, engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution crystal structures of mRuby, the red sensor RCaMP...

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Bibliographic Details
Main Authors:	Jasper eAkerboom, Nicole eCarreras Calderón, Lin eTian, Sebastian eWabnig, Matthias ePrigge, Johan eTolö, Andrew eGordus, Michael B Orger, Kristen E Severi, John J Macklin, Ronak ePatel, Stefan R Pulver, Trevor J Wardill, Elisabeth eFischer, Christina eSchüler, Tsai-Wen eChen, Karen S Sarkisyan, Jonathan S Marvin, Cornelia I Bargmann, Douglas S Kim, Sebastian eKügler, Leon eLagnado, Peter eHegemann, Alexander eGottschalk, Eric R Schreiter, Loren L Looger
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2013-03-01
Series:	Frontiers in Molecular Neuroscience
Subjects:	Protein Structure, Tertiary functional imaging optogenetics Genetically encoded calcium indicators (GECIs) Optogenetic Neuroimaging
Online Access:	http://journal.frontiersin.org/Journal/10.3389/fnmol.2013.00002/full

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