Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning.
The renal stroma is an embryonic cell population located in the cortex that provides a structural framework as well as a source of endothelial progenitors for the developing kidney. The exact role of the renal stroma in normal kidney development hasn't been clearly defined. However, previous st...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3914987?pdf=render |
| _version_ | 1798044561961910272 |
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| author | Stephanie Hum Christopher Rymer Caitlin Schaefer Daniel Bushnell Sunder Sims-Lucas |
| author_facet | Stephanie Hum Christopher Rymer Caitlin Schaefer Daniel Bushnell Sunder Sims-Lucas |
| author_sort | Stephanie Hum |
| collection | DOAJ |
| description | The renal stroma is an embryonic cell population located in the cortex that provides a structural framework as well as a source of endothelial progenitors for the developing kidney. The exact role of the renal stroma in normal kidney development hasn't been clearly defined. However, previous studies have shown that the genetic deletion of Foxd1, a renal stroma specific gene, leads to severe kidney malformations confirming the importance of stroma in normal kidney development. This study further investigates the role of renal stroma by ablating Foxd1-derived stroma cells themselves and observing the response of the remaining cell populations. A Foxd1cre (renal stroma specific) mouse was crossed with a diphtheria toxin mouse (DTA) to specifically induce apoptosis in stromal cells. Histological examination of kidneys at embryonic day 13.5-18.5 showed a lack of stromal tissue, mispatterning of renal structures, and dysplastic and/or fused horseshoe kidneys. Immunofluorescence staining of nephron progenitors, vasculature, ureteric epithelium, differentiated nephron progenitors, and vascular supportive cells revealed that mutants had thickened nephron progenitor caps, cortical regions devoid of nephron progenitors, aberrant vessel patterning and thickening, ureteric branching defects and migration of differentiated nephron structures into the medulla. The similarities between the renal deformities caused by Foxd1 genetic knockout and Foxd1DTA mouse models reveal the importance of Foxd1 in mediating and maintaining the functional integrity of the renal stroma. |
| first_indexed | 2024-04-11T23:07:25Z |
| format | Article |
| id | doaj.art-46e5738733c9492e9ac43b0968a03c13 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T23:07:25Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-46e5738733c9492e9ac43b0968a03c132022-12-22T03:57:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8840010.1371/journal.pone.0088400Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning.Stephanie HumChristopher RymerCaitlin SchaeferDaniel BushnellSunder Sims-LucasThe renal stroma is an embryonic cell population located in the cortex that provides a structural framework as well as a source of endothelial progenitors for the developing kidney. The exact role of the renal stroma in normal kidney development hasn't been clearly defined. However, previous studies have shown that the genetic deletion of Foxd1, a renal stroma specific gene, leads to severe kidney malformations confirming the importance of stroma in normal kidney development. This study further investigates the role of renal stroma by ablating Foxd1-derived stroma cells themselves and observing the response of the remaining cell populations. A Foxd1cre (renal stroma specific) mouse was crossed with a diphtheria toxin mouse (DTA) to specifically induce apoptosis in stromal cells. Histological examination of kidneys at embryonic day 13.5-18.5 showed a lack of stromal tissue, mispatterning of renal structures, and dysplastic and/or fused horseshoe kidneys. Immunofluorescence staining of nephron progenitors, vasculature, ureteric epithelium, differentiated nephron progenitors, and vascular supportive cells revealed that mutants had thickened nephron progenitor caps, cortical regions devoid of nephron progenitors, aberrant vessel patterning and thickening, ureteric branching defects and migration of differentiated nephron structures into the medulla. The similarities between the renal deformities caused by Foxd1 genetic knockout and Foxd1DTA mouse models reveal the importance of Foxd1 in mediating and maintaining the functional integrity of the renal stroma.http://europepmc.org/articles/PMC3914987?pdf=render |
| spellingShingle | Stephanie Hum Christopher Rymer Caitlin Schaefer Daniel Bushnell Sunder Sims-Lucas Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. PLoS ONE |
| title | Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. |
| title_full | Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. |
| title_fullStr | Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. |
| title_full_unstemmed | Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. |
| title_short | Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. |
| title_sort | ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning |
| url | http://europepmc.org/articles/PMC3914987?pdf=render |
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