Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids
ABSTRACTThe spread of hypervirulent (hv) and carbapenem-/multidrug-resistant Klebsiella pneumoniae is an emerging problem in healthcare settings. The New Delhi metallo-β-lactamase-1 (blaNDM-1) is found in Enterobacteriaceae including K. pneumoniae. The blaNDM-1 is capable of hydrolyzing β-lactam ant...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-03-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03017-23 |
| _version_ | 1827329389014024192 |
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| author | Frederick Ofosu-Appiah Ezra E. Acquah Jibril Mohammed Comfort Sakyi Addo Bright Agbodzi Dorcas A. S. Ofosu Charles J. Myers Quaneeta Mohktar Opoku-Ware Ampomah Anthony Ablordey Nana Ama Amissah |
| author_facet | Frederick Ofosu-Appiah Ezra E. Acquah Jibril Mohammed Comfort Sakyi Addo Bright Agbodzi Dorcas A. S. Ofosu Charles J. Myers Quaneeta Mohktar Opoku-Ware Ampomah Anthony Ablordey Nana Ama Amissah |
| author_sort | Frederick Ofosu-Appiah |
| collection | DOAJ |
| description | ABSTRACTThe spread of hypervirulent (hv) and carbapenem-/multidrug-resistant Klebsiella pneumoniae is an emerging problem in healthcare settings. The New Delhi metallo-β-lactamase-1 (blaNDM-1) is found in Enterobacteriaceae including K. pneumoniae. The blaNDM-1 is capable of hydrolyzing β-lactam antibiotics which are used for treatment of severe infections caused by multidrug-resistant Gram-negative bacteria. This is associated with the unacceptably high mortality rate in immunocompromised burn injury patients. This study reports on the characterization of blaNDM-1 gene and virulence factors in hv carbapenem-/multidrug-resistant K. pneumoniae ST147 in the burns unit of a tertiary teaching hospital during routine surveillance. Two K. pneumoniae strains were obtained from wounds of burn-infected patients from May 2020 to July 2021. The hypervirulence genes and genetic context of the blaNDM-1 gene and mobile genetic elements potentially involved in the transposition of the gene were analyzed. We identified a conserved genetic background and an IS26 and open reading frame flanking the blaNDM-1 gene that could suggest its involvement in the mobilization of the gene. The plasmid harbored additional antibiotic resistance predicted regions that were responsible for resistance to almost all the routinely used antibiotics. To ensure the identification of potential outbreak strains during routine surveillance, investigations on resistance genes and their environment in relation to evolution are necessary for molecular epidemiology.IMPORTANCEData obtained from this study will aid in the prompt identification of disease outbreaks including evolving resistance and virulence of the outbreak bacteria. This will help establish and implement antimicrobial stewardship programs and infection prevention protocols in fragile health systems in countries with limited resources. Integration of molecular surveillance and translation of whole-genome sequencing in routine diagnosis will provide valuable data for control of infection. This study reports for the first time a high-risk clone K. pneumoniae ST147 with hypervirulence and multidrug-resistance features in Ghana. |
| first_indexed | 2024-03-07T15:33:31Z |
| format | Article |
| id | doaj.art-46e5eedea87648a9a7a0caef7515ee64 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-07T15:33:31Z |
| publishDate | 2024-03-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-46e5eedea87648a9a7a0caef7515ee642024-03-05T14:04:36ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-03-0112310.1128/spectrum.03017-23Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmidsFrederick Ofosu-Appiah0Ezra E. Acquah1Jibril Mohammed2Comfort Sakyi Addo3Bright Agbodzi4Dorcas A. S. Ofosu5Charles J. Myers6Quaneeta Mohktar7Opoku-Ware Ampomah8Anthony Ablordey9Nana Ama Amissah10West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaThe Burns Unit, Reconstructive Plastic Surgery and Burns Unit, Korle Bu Teaching Hospital, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaDepartment of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, GhanaABSTRACTThe spread of hypervirulent (hv) and carbapenem-/multidrug-resistant Klebsiella pneumoniae is an emerging problem in healthcare settings. The New Delhi metallo-β-lactamase-1 (blaNDM-1) is found in Enterobacteriaceae including K. pneumoniae. The blaNDM-1 is capable of hydrolyzing β-lactam antibiotics which are used for treatment of severe infections caused by multidrug-resistant Gram-negative bacteria. This is associated with the unacceptably high mortality rate in immunocompromised burn injury patients. This study reports on the characterization of blaNDM-1 gene and virulence factors in hv carbapenem-/multidrug-resistant K. pneumoniae ST147 in the burns unit of a tertiary teaching hospital during routine surveillance. Two K. pneumoniae strains were obtained from wounds of burn-infected patients from May 2020 to July 2021. The hypervirulence genes and genetic context of the blaNDM-1 gene and mobile genetic elements potentially involved in the transposition of the gene were analyzed. We identified a conserved genetic background and an IS26 and open reading frame flanking the blaNDM-1 gene that could suggest its involvement in the mobilization of the gene. The plasmid harbored additional antibiotic resistance predicted regions that were responsible for resistance to almost all the routinely used antibiotics. To ensure the identification of potential outbreak strains during routine surveillance, investigations on resistance genes and their environment in relation to evolution are necessary for molecular epidemiology.IMPORTANCEData obtained from this study will aid in the prompt identification of disease outbreaks including evolving resistance and virulence of the outbreak bacteria. This will help establish and implement antimicrobial stewardship programs and infection prevention protocols in fragile health systems in countries with limited resources. Integration of molecular surveillance and translation of whole-genome sequencing in routine diagnosis will provide valuable data for control of infection. This study reports for the first time a high-risk clone K. pneumoniae ST147 with hypervirulence and multidrug-resistance features in Ghana.https://journals.asm.org/doi/10.1128/spectrum.03017-23Klebsiella pneumoniaemultidrug resistanceblaNDM-1 genehypervirulencemobile genetic elementsburn wounds |
| spellingShingle | Frederick Ofosu-Appiah Ezra E. Acquah Jibril Mohammed Comfort Sakyi Addo Bright Agbodzi Dorcas A. S. Ofosu Charles J. Myers Quaneeta Mohktar Opoku-Ware Ampomah Anthony Ablordey Nana Ama Amissah Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids Microbiology Spectrum Klebsiella pneumoniae multidrug resistance blaNDM-1 gene hypervirulence mobile genetic elements burn wounds |
| title | Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids |
| title_full | Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids |
| title_fullStr | Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids |
| title_full_unstemmed | Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids |
| title_short | Klebsiella pneumoniae ST147 harboring blaNDM-1, multidrug resistance and hypervirulence plasmids |
| title_sort | klebsiella pneumoniae st147 harboring blandm 1 multidrug resistance and hypervirulence plasmids |
| topic | Klebsiella pneumoniae multidrug resistance blaNDM-1 gene hypervirulence mobile genetic elements burn wounds |
| url | https://journals.asm.org/doi/10.1128/spectrum.03017-23 |
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