TREC/KREC Levels in Young COVID-19 Patients
COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/8/1486 |
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| author | Maryam B. Khadzhieva Ekaterina V. Kalinina Sergey S. Larin Daria A. Sviridova Alesya S. Gracheva Julia V. Chursinova Vadim A. Stepanov Ivan V. Redkin Lyudmila S. Avdeikina Alexander G. Rumyantsev Artem N. Kuzovlev Lyubov E. Salnikova |
| author_facet | Maryam B. Khadzhieva Ekaterina V. Kalinina Sergey S. Larin Daria A. Sviridova Alesya S. Gracheva Julia V. Chursinova Vadim A. Stepanov Ivan V. Redkin Lyudmila S. Avdeikina Alexander G. Rumyantsev Artem N. Kuzovlev Lyubov E. Salnikova |
| author_sort | Maryam B. Khadzhieva |
| collection | DOAJ |
| description | COVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R<sup>2</sup> = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19. |
| first_indexed | 2024-03-10T08:52:30Z |
| format | Article |
| id | doaj.art-46e84088bed041c59782b981e2c10db8 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T08:52:30Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-46e84088bed041c59782b981e2c10db82023-11-22T07:21:11ZengMDPI AGDiagnostics2075-44182021-08-01118148610.3390/diagnostics11081486TREC/KREC Levels in Young COVID-19 PatientsMaryam B. Khadzhieva0Ekaterina V. Kalinina1Sergey S. Larin2Daria A. Sviridova3Alesya S. Gracheva4Julia V. Chursinova5Vadim A. Stepanov6Ivan V. Redkin7Lyudmila S. Avdeikina8Alexander G. Rumyantsev9Artem N. Kuzovlev10Lyubov E. Salnikova11Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaDmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Moscow, RussiaDmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Moscow, RussiaVavilov Institute of General Genetics, Russian Academy of Sciences, 119991 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaM.F. Vladimirsky Moscow Regional Research and Clinical Institute, 129110 Moscow, RussiaM.F. Vladimirsky Moscow Regional Research and Clinical Institute, 129110 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaMoscow Clinical Center for Infectious Diseases “Voronovskoe”, 142160 Moscow, RussiaDmitry Rogachev National Research Center of Pediatric Hematology, Oncology and Immunology, 117997 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaCOVID-19 patients with acute respiratory distress syndrome (ARDS) have an immune imbalance when systemic inflammation and dysfunction of circulating T and B cells lead to a more severe disease. Using TREC/KREC analysis, we studied the level of mature naive T and B cells in peripheral blood of COVID-19 patients and its relationship with clinical and laboratory data. TREC/KREC analysis was performed by multiplex real-time quantitative PCR on a sample of 36 patients aged 45 years or younger. The reduced TREC/KREC level was observed in ARDS patients compared with non-ARDS patients, and similar results were found for the deceased patients. During days 6 to 20 of hospitalization, a higher neutrophil-to-lymphocyte ratio (NLR) was detected in ARDS patients compared with non-ARDS patients. TREC/KREC negatively correlated with NLR; the highest correlation was recorded for TREC per 100,000 cells with the coefficient of determination R<sup>2</sup> = 0.527. Thus, TREC/KREC analysis is a potential prognostic marker for assessing the severity and outcome in COVID-19.https://www.mdpi.com/2075-4418/11/8/1486COVID-19acute respiratory distress syndromeTREC/KRECneutrophil-to-lymphocyte ratio |
| spellingShingle | Maryam B. Khadzhieva Ekaterina V. Kalinina Sergey S. Larin Daria A. Sviridova Alesya S. Gracheva Julia V. Chursinova Vadim A. Stepanov Ivan V. Redkin Lyudmila S. Avdeikina Alexander G. Rumyantsev Artem N. Kuzovlev Lyubov E. Salnikova TREC/KREC Levels in Young COVID-19 Patients Diagnostics COVID-19 acute respiratory distress syndrome TREC/KREC neutrophil-to-lymphocyte ratio |
| title | TREC/KREC Levels in Young COVID-19 Patients |
| title_full | TREC/KREC Levels in Young COVID-19 Patients |
| title_fullStr | TREC/KREC Levels in Young COVID-19 Patients |
| title_full_unstemmed | TREC/KREC Levels in Young COVID-19 Patients |
| title_short | TREC/KREC Levels in Young COVID-19 Patients |
| title_sort | trec krec levels in young covid 19 patients |
| topic | COVID-19 acute respiratory distress syndrome TREC/KREC neutrophil-to-lymphocyte ratio |
| url | https://www.mdpi.com/2075-4418/11/8/1486 |
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