Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin
A double-channel diagnostic fluorimeter operating in pulsed mode for in vivo assessment of the content of advanced glycation end products (AGEs) in skin by the autofluorescence integral intensity in the visible spectral region is developed. To excite the fluorescence we use a light-emitting diode wi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Samara National Research University
2018-03-01
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| Series: | Journal of Biomedical Photonics & Engineering |
| Subjects: | |
| Online Access: | http://jbpe.ssau.ru/index.php/JBPE/article/view/3274 |
| _version_ | 1818155825064050688 |
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| author | Vladimir N. Grishanov Victor S. Kulikov Konstantin V. Cherepanov |
| author_facet | Vladimir N. Grishanov Victor S. Kulikov Konstantin V. Cherepanov |
| author_sort | Vladimir N. Grishanov |
| collection | DOAJ |
| description | A double-channel diagnostic fluorimeter operating in pulsed mode for in vivo assessment of the content of advanced glycation end products (AGEs) in skin by the autofluorescence integral intensity in the visible spectral region is developed. To excite the fluorescence we use a light-emitting diode with the peak wavelength 365 nm. A green light-emitting diode and an additional photodetection channel are intended to allow for the skin phototype of the patient. The fluorimeter analogue electronics comprises two photodetection channels based on silicon photodiodes. The accepted noise-suppressing circuitry solutions reduce the effect of sun and illumination lamp background light on the digital signal at the output of the 10-bit ADC to the one-bit level in both channels. The digital part of the fluorimeter is based on the Arduino platform. The software controls the operation modes of the fluorimeter, provides the quantitative processing of results, the diagnostic data storage and visualization. The experimental studies demonstrated the capabilities of revealing age-related changes in the skin. |
| first_indexed | 2024-12-11T14:48:33Z |
| format | Article |
| id | doaj.art-46e8be171909454eb6713ea2db4bdca1 |
| institution | Directory Open Access Journal |
| issn | 2411-2844 |
| language | English |
| last_indexed | 2024-12-11T14:48:33Z |
| publishDate | 2018-03-01 |
| publisher | Samara National Research University |
| record_format | Article |
| series | Journal of Biomedical Photonics & Engineering |
| spelling | doaj.art-46e8be171909454eb6713ea2db4bdca12022-12-22T01:01:34ZengSamara National Research UniversityJournal of Biomedical Photonics & Engineering2411-28442018-03-014110.18287/JBPE18.04.0105062967Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skinVladimir N. Grishanov0Victor S. Kulikov1Konstantin V. Cherepanov2Samara National Research University, RussiaSamara National Research University, RussiaSamara National Research University, RussiaA double-channel diagnostic fluorimeter operating in pulsed mode for in vivo assessment of the content of advanced glycation end products (AGEs) in skin by the autofluorescence integral intensity in the visible spectral region is developed. To excite the fluorescence we use a light-emitting diode with the peak wavelength 365 nm. A green light-emitting diode and an additional photodetection channel are intended to allow for the skin phototype of the patient. The fluorimeter analogue electronics comprises two photodetection channels based on silicon photodiodes. The accepted noise-suppressing circuitry solutions reduce the effect of sun and illumination lamp background light on the digital signal at the output of the 10-bit ADC to the one-bit level in both channels. The digital part of the fluorimeter is based on the Arduino platform. The software controls the operation modes of the fluorimeter, provides the quantitative processing of results, the diagnostic data storage and visualization. The experimental studies demonstrated the capabilities of revealing age-related changes in the skin.http://jbpe.ssau.ru/index.php/JBPE/article/view/3274skin autofluorescencefluorimeteradvanced glycation end productsmedical diagnostics |
| spellingShingle | Vladimir N. Grishanov Victor S. Kulikov Konstantin V. Cherepanov Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin Journal of Biomedical Photonics & Engineering skin autofluorescence fluorimeter advanced glycation end products medical diagnostics |
| title | Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| title_full | Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| title_fullStr | Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| title_full_unstemmed | Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| title_short | Double-channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| title_sort | double channel fluorimeter with pulsed excitation of advanced glycation end products in skin |
| topic | skin autofluorescence fluorimeter advanced glycation end products medical diagnostics |
| url | http://jbpe.ssau.ru/index.php/JBPE/article/view/3274 |
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