Genome-enabled discovery of evolutionary divergence in brains and behavior
Abstract Lake Malawi cichlid fishes exhibit extensive divergence in form and function built from a relatively small number of genetic changes. We compared the genomes of rock- and sand-dwelling species and asked which genetic variants differed among the groups. We found that 96% of differentiated va...
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-92385-8 |
| _version_ | 1818839206751895552 |
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| author | Chinar Patil Jonathan B. Sylvester Kawther Abdilleh Michael W. Norsworthy Karen Pottin Milan Malinsky Ryan F. Bloomquist Zachary V. Johnson Patrick T. McGrath Jeffrey T. Streelman |
| author_facet | Chinar Patil Jonathan B. Sylvester Kawther Abdilleh Michael W. Norsworthy Karen Pottin Milan Malinsky Ryan F. Bloomquist Zachary V. Johnson Patrick T. McGrath Jeffrey T. Streelman |
| author_sort | Chinar Patil |
| collection | DOAJ |
| description | Abstract Lake Malawi cichlid fishes exhibit extensive divergence in form and function built from a relatively small number of genetic changes. We compared the genomes of rock- and sand-dwelling species and asked which genetic variants differed among the groups. We found that 96% of differentiated variants reside in non-coding sequence but these non-coding diverged variants are evolutionarily conserved. Genome regions near differentiated variants are enriched for craniofacial, neural and behavioral categories. Following leads from genome sequence, we used rock- vs. sand-species and their hybrids to (i) delineate the push–pull roles of BMP signaling and irx1b in the specification of forebrain territories during gastrulation and (ii) reveal striking context-dependent brain gene expression during adult social behavior. Our results demonstrate how divergent genome sequences can predict differences in key evolutionary traits. We highlight the promise of evolutionary reverse genetics—the inference of phenotypic divergence from unbiased genome sequencing and then empirical validation in natural populations. |
| first_indexed | 2024-12-19T03:50:37Z |
| format | Article |
| id | doaj.art-46eae42d2f5b43029dbc177bba9c934c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T03:50:37Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-46eae42d2f5b43029dbc177bba9c934c2022-12-21T20:37:00ZengNature PortfolioScientific Reports2045-23222021-06-0111111410.1038/s41598-021-92385-8Genome-enabled discovery of evolutionary divergence in brains and behaviorChinar Patil0Jonathan B. Sylvester1Kawther Abdilleh2Michael W. Norsworthy3Karen Pottin4Milan Malinsky5Ryan F. Bloomquist6Zachary V. Johnson7Patrick T. McGrath8Jeffrey T. Streelman9School of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologySchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologySchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologyCatalog Technologies Inc.Laboratoire de Biologie du Dévelopement (IBPS-LBD, UMR7622), CNRS, Institut de Biologie Paris Seine, Sorbonne UniversitéDepartment of Environmental Sciences, Zoological Institute, University of BaselSchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologySchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologySchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologySchool of Biological Sciences and Petit Institute of Bioengineering and Bioscience, Georgia Institute of TechnologyAbstract Lake Malawi cichlid fishes exhibit extensive divergence in form and function built from a relatively small number of genetic changes. We compared the genomes of rock- and sand-dwelling species and asked which genetic variants differed among the groups. We found that 96% of differentiated variants reside in non-coding sequence but these non-coding diverged variants are evolutionarily conserved. Genome regions near differentiated variants are enriched for craniofacial, neural and behavioral categories. Following leads from genome sequence, we used rock- vs. sand-species and their hybrids to (i) delineate the push–pull roles of BMP signaling and irx1b in the specification of forebrain territories during gastrulation and (ii) reveal striking context-dependent brain gene expression during adult social behavior. Our results demonstrate how divergent genome sequences can predict differences in key evolutionary traits. We highlight the promise of evolutionary reverse genetics—the inference of phenotypic divergence from unbiased genome sequencing and then empirical validation in natural populations.https://doi.org/10.1038/s41598-021-92385-8 |
| spellingShingle | Chinar Patil Jonathan B. Sylvester Kawther Abdilleh Michael W. Norsworthy Karen Pottin Milan Malinsky Ryan F. Bloomquist Zachary V. Johnson Patrick T. McGrath Jeffrey T. Streelman Genome-enabled discovery of evolutionary divergence in brains and behavior Scientific Reports |
| title | Genome-enabled discovery of evolutionary divergence in brains and behavior |
| title_full | Genome-enabled discovery of evolutionary divergence in brains and behavior |
| title_fullStr | Genome-enabled discovery of evolutionary divergence in brains and behavior |
| title_full_unstemmed | Genome-enabled discovery of evolutionary divergence in brains and behavior |
| title_short | Genome-enabled discovery of evolutionary divergence in brains and behavior |
| title_sort | genome enabled discovery of evolutionary divergence in brains and behavior |
| url | https://doi.org/10.1038/s41598-021-92385-8 |
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