Cell-Free Protein Synthesis: Chassis toward the Minimal Cell
The quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology. Minimal cell projects came from the study of a plausible route to the origin of life. Later on, research extended and also r...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-04-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/8/4/315 |
| _version_ | 1797724902480936960 |
|---|---|
| author | Ke Yue Yiyong Zhu Lei Kai |
| author_facet | Ke Yue Yiyong Zhu Lei Kai |
| author_sort | Ke Yue |
| collection | DOAJ |
| description | The quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology. Minimal cell projects came from the study of a plausible route to the origin of life. Later on, research extended and also referred to the construction of artificial cells, or even more broadly, as in vitro synthetic biology. The cell-free protein synthesis (CFPS) techniques harness the central cellular activity of transcription/translation in an open environment, providing the framework for multiple cellular processes assembling. Therefore, CFPS systems have become the first choice in the construction of the minimal cell. In this review, we focus on the recent advances in the quantitative analysis of CFPS and on its advantage for addressing the bottom-up assembly of a minimal cell and illustrate the importance of systemic chassis behavior, such as stochasticity under a compartmentalized micro-environment. |
| first_indexed | 2024-03-12T10:23:35Z |
| format | Article |
| id | doaj.art-46eb1e9757494dcd956d5e3282a43dd3 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:23:35Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-46eb1e9757494dcd956d5e3282a43dd32023-09-02T09:54:40ZengMDPI AGCells2073-44092019-04-018431510.3390/cells8040315cells8040315Cell-Free Protein Synthesis: Chassis toward the Minimal CellKe Yue0Yiyong Zhu1Lei Kai2The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Shanghai Road 101, Xuzhou 221116, ChinaJiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing 210095, ChinaThe Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Shanghai Road 101, Xuzhou 221116, ChinaThe quest for a minimal cell not only sheds light on the fundamental principles of life but also brings great advances in related applied fields such as general biotechnology. Minimal cell projects came from the study of a plausible route to the origin of life. Later on, research extended and also referred to the construction of artificial cells, or even more broadly, as in vitro synthetic biology. The cell-free protein synthesis (CFPS) techniques harness the central cellular activity of transcription/translation in an open environment, providing the framework for multiple cellular processes assembling. Therefore, CFPS systems have become the first choice in the construction of the minimal cell. In this review, we focus on the recent advances in the quantitative analysis of CFPS and on its advantage for addressing the bottom-up assembly of a minimal cell and illustrate the importance of systemic chassis behavior, such as stochasticity under a compartmentalized micro-environment.https://www.mdpi.com/2073-4409/8/4/315cell-free protein synthesischassisgene circuitsmicro-compartmentsstochasticity |
| spellingShingle | Ke Yue Yiyong Zhu Lei Kai Cell-Free Protein Synthesis: Chassis toward the Minimal Cell Cells cell-free protein synthesis chassis gene circuits micro-compartments stochasticity |
| title | Cell-Free Protein Synthesis: Chassis toward the Minimal Cell |
| title_full | Cell-Free Protein Synthesis: Chassis toward the Minimal Cell |
| title_fullStr | Cell-Free Protein Synthesis: Chassis toward the Minimal Cell |
| title_full_unstemmed | Cell-Free Protein Synthesis: Chassis toward the Minimal Cell |
| title_short | Cell-Free Protein Synthesis: Chassis toward the Minimal Cell |
| title_sort | cell free protein synthesis chassis toward the minimal cell |
| topic | cell-free protein synthesis chassis gene circuits micro-compartments stochasticity |
| url | https://www.mdpi.com/2073-4409/8/4/315 |
| work_keys_str_mv | AT keyue cellfreeproteinsynthesischassistowardtheminimalcell AT yiyongzhu cellfreeproteinsynthesischassistowardtheminimalcell AT leikai cellfreeproteinsynthesischassistowardtheminimalcell |