Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin
<i>Ferulopsis hystrix</i> is a perennial plant of the Apiaceae family. In Buryat and Mongolian medicine, it is used as a substitute for <i>Costus speciosus</i> roots (ru rta), and in Tibetan medicine, it is used to cure digestive system diseases and used as a wound-healing ag...
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MDPI AG
2023-08-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/17/9653 |
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| author | Yanina G. Razuvaeva Anyuta A. Toropova Saizana M. Salchak Daniil N. Olennikov |
| author_facet | Yanina G. Razuvaeva Anyuta A. Toropova Saizana M. Salchak Daniil N. Olennikov |
| author_sort | Yanina G. Razuvaeva |
| collection | DOAJ |
| description | <i>Ferulopsis hystrix</i> is a perennial plant of the Apiaceae family. In Buryat and Mongolian medicine, it is used as a substitute for <i>Costus speciosus</i> roots (ru rta), and in Tibetan medicine, it is used to cure digestive system diseases and used as a wound-healing agent. However, its metabolites and their bioactivities are still poorly understood. High-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass-spectrometric detection (HPLC–PDA–ESI–tQ–MS/MS) were used to identify approximately 42 phenolic compounds in <i>F. hystrix</i>, and 30 coumarins were characterized and quantified. The major compounds in <i>F. hystrix</i> roots were skimmin (umbelliferone 7-<i>O</i>-glucoside) and peucenidin (vaginidiol 3′-<i>O</i>-acetyl-4′-<i>O</i>-senecioyl ester), of which, gastroprotective and antioxidant effects were found for the first time. The abovementioned compounds exhibit a gastroprotective effect against indomethacin and steroid gastropathy by reducing the amount of damage (point, large, and strip-like erosions) in the gastric mucosa and lowering the corresponding Paul’s index. The most pronounced gastroprotective effect was exhibited by skimmin at a dose of 1 mg/kg and by peucenidin at doses of 16 and 48 mg/kg; these compounds help to limit the development of pronounced erosive–necrotic processes in the gastric mucosa. In pathological conditions, these compounds reduce malondialdehyde, increase the activity of catalase, and increase the content of reduced glutathione in the blood. Thus, this study demonstrates that <i>F. hystrix</i> roots are a good source of bioactive coumarins with gastroprotective potential, which supports earlier ethnopharmacological studies. |
| first_indexed | 2024-03-10T23:27:34Z |
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| last_indexed | 2024-03-10T23:27:34Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-46ebb2048bab443db03b8e66e3f03de42023-11-19T07:49:59ZengMDPI AGApplied Sciences2076-34172023-08-011317965310.3390/app13179653Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and PeucenidinYanina G. Razuvaeva0Anyuta A. Toropova1Saizana M. Salchak2Daniil N. Olennikov3Biologically Active Substances Safety Laboratory, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakhyanovoy Street, 670047 Ulan-Ude, RussiaBiologically Active Substances Safety Laboratory, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakhyanovoy Street, 670047 Ulan-Ude, RussiaBiologically Active Substances Safety Laboratory, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakhyanovoy Street, 670047 Ulan-Ude, RussiaLaboratory of Biomedical Research, Institute of General and Experimental Biology, Siberian Division, Russian Academy of Science, 6 Sakhyanovoy Street, 670047 Ulan-Ude, Russia<i>Ferulopsis hystrix</i> is a perennial plant of the Apiaceae family. In Buryat and Mongolian medicine, it is used as a substitute for <i>Costus speciosus</i> roots (ru rta), and in Tibetan medicine, it is used to cure digestive system diseases and used as a wound-healing agent. However, its metabolites and their bioactivities are still poorly understood. High-performance liquid chromatography with photodiode array detection and electrospray ionization triple quadrupole mass-spectrometric detection (HPLC–PDA–ESI–tQ–MS/MS) were used to identify approximately 42 phenolic compounds in <i>F. hystrix</i>, and 30 coumarins were characterized and quantified. The major compounds in <i>F. hystrix</i> roots were skimmin (umbelliferone 7-<i>O</i>-glucoside) and peucenidin (vaginidiol 3′-<i>O</i>-acetyl-4′-<i>O</i>-senecioyl ester), of which, gastroprotective and antioxidant effects were found for the first time. The abovementioned compounds exhibit a gastroprotective effect against indomethacin and steroid gastropathy by reducing the amount of damage (point, large, and strip-like erosions) in the gastric mucosa and lowering the corresponding Paul’s index. The most pronounced gastroprotective effect was exhibited by skimmin at a dose of 1 mg/kg and by peucenidin at doses of 16 and 48 mg/kg; these compounds help to limit the development of pronounced erosive–necrotic processes in the gastric mucosa. In pathological conditions, these compounds reduce malondialdehyde, increase the activity of catalase, and increase the content of reduced glutathione in the blood. Thus, this study demonstrates that <i>F. hystrix</i> roots are a good source of bioactive coumarins with gastroprotective potential, which supports earlier ethnopharmacological studies.https://www.mdpi.com/2076-3417/13/17/9653<i>Ferulopsis hystrix</i>coumarinsskimminpeucenidinLC–MSgastroptotective activity |
| spellingShingle | Yanina G. Razuvaeva Anyuta A. Toropova Saizana M. Salchak Daniil N. Olennikov Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin Applied Sciences <i>Ferulopsis hystrix</i> coumarins skimmin peucenidin LC–MS gastroptotective activity |
| title | Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin |
| title_full | Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin |
| title_fullStr | Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin |
| title_full_unstemmed | Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin |
| title_short | Coumarins of <i>Ferulopsis hystrix</i>: LC–MS Profiling and Gastroprotective and Antioxidant Activities of Skimmin and Peucenidin |
| title_sort | coumarins of i ferulopsis hystrix i lc ms profiling and gastroprotective and antioxidant activities of skimmin and peucenidin |
| topic | <i>Ferulopsis hystrix</i> coumarins skimmin peucenidin LC–MS gastroptotective activity |
| url | https://www.mdpi.com/2076-3417/13/17/9653 |
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