A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering
In this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a lar...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/3/3/26 |
| _version_ | 1819171819810193408 |
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| author | Ivan Hernandez Alok Kumar Binata Joddar |
| author_facet | Ivan Hernandez Alok Kumar Binata Joddar |
| author_sort | Ivan Hernandez |
| collection | DOAJ |
| description | In this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a larger amount of hydrogel to be loaded within the scaffolds as compared to 3D printed mesh and honeycomb scaffolds of similar volumes and strut thicknesses. The hydrogel was a mixture of alginate, gelatin, and nano-hydroxyapatite, infiltrated with human mesenchymal stem cells (hMSC) to enhance the osteoconductivity and biocompatibility of the system. Adhesion and viability of hMSC in the PCL/hydrogel system confirmed its cytocompatibility. Biomineralization tests in simulated body fluid (SBF) showed the nucleation and growth of apatite crystals, which confirmed the bioactivity of the PCL/hydrogel system. Moreover, dissolution studies, in SBF revealed a sustained dissolution of the hydrogel with time. Overall, the present study provides a new approach in bone tissue engineering to repair bone defects with a bioactive hybrid system consisting of a polymeric scaffold, hydrogel, and hMSC. |
| first_indexed | 2024-12-22T19:57:21Z |
| format | Article |
| id | doaj.art-46f12760ee444e69a608b122854b5ce4 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-12-22T19:57:21Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-46f12760ee444e69a608b122854b5ce42022-12-21T18:14:24ZengMDPI AGGels2310-28612017-07-01332610.3390/gels3030026gels3030026A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue EngineeringIvan Hernandez0Alok Kumar1Binata Joddar2Inspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USAInspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USAInspired Materials & Stem-Cell Based Tissue Engineering Laboratory (IMSTEL), Department of Metallurgical, Materials and Biomedical Engineering, University of Texas at El Paso, El Paso, TX 79968, USAIn this study, a hybrid system consisting of 3D printed polycaprolactone (PCL) filled with hydrogel was developed as an application for reconstruction of long bone defects, which are innately difficult to repair due to large missing segments of bone. A 3D printed gyroid scaffold of PCL allowed a larger amount of hydrogel to be loaded within the scaffolds as compared to 3D printed mesh and honeycomb scaffolds of similar volumes and strut thicknesses. The hydrogel was a mixture of alginate, gelatin, and nano-hydroxyapatite, infiltrated with human mesenchymal stem cells (hMSC) to enhance the osteoconductivity and biocompatibility of the system. Adhesion and viability of hMSC in the PCL/hydrogel system confirmed its cytocompatibility. Biomineralization tests in simulated body fluid (SBF) showed the nucleation and growth of apatite crystals, which confirmed the bioactivity of the PCL/hydrogel system. Moreover, dissolution studies, in SBF revealed a sustained dissolution of the hydrogel with time. Overall, the present study provides a new approach in bone tissue engineering to repair bone defects with a bioactive hybrid system consisting of a polymeric scaffold, hydrogel, and hMSC.https://www.mdpi.com/2310-2861/3/3/263D printingpolycaprolactone (PCL)hydroxyapatitehydrogelbone defect |
| spellingShingle | Ivan Hernandez Alok Kumar Binata Joddar A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering Gels 3D printing polycaprolactone (PCL) hydroxyapatite hydrogel bone defect |
| title | A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering |
| title_full | A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering |
| title_fullStr | A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering |
| title_full_unstemmed | A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering |
| title_short | A Bioactive Hydrogel and 3D Printed Polycaprolactone System for Bone Tissue Engineering |
| title_sort | bioactive hydrogel and 3d printed polycaprolactone system for bone tissue engineering |
| topic | 3D printing polycaprolactone (PCL) hydroxyapatite hydrogel bone defect |
| url | https://www.mdpi.com/2310-2861/3/3/26 |
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