Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant
Abstract Influenza poses a severe threat to global health. Despite the whole inactivated virus (WIV)‐based nasal vaccine being a promising strategy for influenza protection, the mucosal barrier is still a bottleneck of the nasal vaccine. Here, a catalytic mucosal adjuvant strategy for an influenza W...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-09-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202000771 |
| _version_ | 1828849339156398080 |
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| author | Tao Qin Shang Ma Xinyu Miao Yan Tang Dandan Huangfu Jinyuan Wang Jing Jiang Nuo Xu Yuncong Yin Sujuan Chen Xiufan Liu Yinyan Yin Daxin Peng Lizeng Gao |
| author_facet | Tao Qin Shang Ma Xinyu Miao Yan Tang Dandan Huangfu Jinyuan Wang Jing Jiang Nuo Xu Yuncong Yin Sujuan Chen Xiufan Liu Yinyan Yin Daxin Peng Lizeng Gao |
| author_sort | Tao Qin |
| collection | DOAJ |
| description | Abstract Influenza poses a severe threat to global health. Despite the whole inactivated virus (WIV)‐based nasal vaccine being a promising strategy for influenza protection, the mucosal barrier is still a bottleneck of the nasal vaccine. Here, a catalytic mucosal adjuvant strategy for an influenza WIV nasal vaccine based on chitosan (CS) functionalized iron oxide nanozyme (IONzyme) is developed. The results reveal that CS‐IONzyme increases antigen adhesion to nasal mucosa by 30‐fold compared to H1N1 WIV alone. Next, CS‐IONzyme facilitates H1N1 WIV to enhance CCL20‐driven submucosal dendritic cell (DC) recruitment and transepithelial dendrite(TED) formation for viral uptake via the toll‐like receptor(TLR) 2/4‐dependent pathway. Moreover, IONzyme with enhanced peroxidase (POD)‐like activity by CS modification catalyzes a reactive oxygen species (ROS)‐dependent DC maturation, which further enhances the migration of H1N1 WIV‐loaded DCs into the draining lymph nodes for antigen presentation. Finally, CS‐IONzyme‐based nasal vaccine triggers an 8.9‐fold increase of IgA‐mucosal adaptive immunity in mice, which provides a 100% protection against influenza, while only a 30% protection by H1N1 WIV alone. This work provides an antiviral alternative for designing nasal vaccines based on IONzyme to combat influenza infection. |
| first_indexed | 2024-12-12T22:50:06Z |
| format | Article |
| id | doaj.art-46f24d61e93a45eea5e3c61229aa7d2d |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-12T22:50:06Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-46f24d61e93a45eea5e3c61229aa7d2d2022-12-22T00:09:06ZengWileyAdvanced Science2198-38442020-09-01718n/an/a10.1002/advs.202000771Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐AdjuvantTao Qin0Shang Ma1Xinyu Miao2Yan Tang3Dandan Huangfu4Jinyuan Wang5Jing Jiang6Nuo Xu7Yuncong Yin8Sujuan Chen9Xiufan Liu10Yinyan Yin11Daxin Peng12Lizeng Gao13College of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaCollege of Veterinary Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaInstitute for Translational Medicine, School of Medicine Yangzhou University Yangzhou Jiangsu 225009 P. R. ChinaAbstract Influenza poses a severe threat to global health. Despite the whole inactivated virus (WIV)‐based nasal vaccine being a promising strategy for influenza protection, the mucosal barrier is still a bottleneck of the nasal vaccine. Here, a catalytic mucosal adjuvant strategy for an influenza WIV nasal vaccine based on chitosan (CS) functionalized iron oxide nanozyme (IONzyme) is developed. The results reveal that CS‐IONzyme increases antigen adhesion to nasal mucosa by 30‐fold compared to H1N1 WIV alone. Next, CS‐IONzyme facilitates H1N1 WIV to enhance CCL20‐driven submucosal dendritic cell (DC) recruitment and transepithelial dendrite(TED) formation for viral uptake via the toll‐like receptor(TLR) 2/4‐dependent pathway. Moreover, IONzyme with enhanced peroxidase (POD)‐like activity by CS modification catalyzes a reactive oxygen species (ROS)‐dependent DC maturation, which further enhances the migration of H1N1 WIV‐loaded DCs into the draining lymph nodes for antigen presentation. Finally, CS‐IONzyme‐based nasal vaccine triggers an 8.9‐fold increase of IgA‐mucosal adaptive immunity in mice, which provides a 100% protection against influenza, while only a 30% protection by H1N1 WIV alone. This work provides an antiviral alternative for designing nasal vaccines based on IONzyme to combat influenza infection.https://doi.org/10.1002/advs.202000771adjuvantinfluenza virusmucosal vaccinenanozyme |
| spellingShingle | Tao Qin Shang Ma Xinyu Miao Yan Tang Dandan Huangfu Jinyuan Wang Jing Jiang Nuo Xu Yuncong Yin Sujuan Chen Xiufan Liu Yinyan Yin Daxin Peng Lizeng Gao Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant Advanced Science adjuvant influenza virus mucosal vaccine nanozyme |
| title | Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant |
| title_full | Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant |
| title_fullStr | Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant |
| title_full_unstemmed | Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant |
| title_short | Mucosal Vaccination for Influenza Protection Enhanced by Catalytic Immune‐Adjuvant |
| title_sort | mucosal vaccination for influenza protection enhanced by catalytic immune adjuvant |
| topic | adjuvant influenza virus mucosal vaccine nanozyme |
| url | https://doi.org/10.1002/advs.202000771 |
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