High-fidelity SaCas9 identified by directional screening in human cells.

High-fidelity SaCas9 identified by directional screening in human cells.

CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes. However, off-target effects remain a major bottleneck that precludes safe and reliable applications in genome editing. Here, we characterize the off-target effects...

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Main Authors: Haihua Xie, Xianglian Ge, Fayu Yang, Bang Wang, Shuang Li, Jinzhi Duan, Xiujuan Lv, Congsheng Cheng, Zongming Song, Changbao Liu, Junzhao Zhao, Yu Zhang, Jinyu Wu, Caixia Gao, Jinwei Zhang, Feng Gu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-07-01
Series:PLoS Biology
Online Access:https://doi.org/10.1371/journal.pbio.3000747

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