FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis
Cuprotosis is a recently identified cell death form that caused by intracellular copper accumulation and regulated by FDX1. This work aimed to explore the role of cuprotosis and the pivotal regulatory gene FDX1 in thyroid cancer development. We observed that expression of FDX1 in tumor section was n...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023058632 |
| _version_ | 1827856211977961472 |
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| author | Gaoxiang Chen Jianan Zhang Weifeng Teng Yong Luo Xiaochun Ji |
| author_facet | Gaoxiang Chen Jianan Zhang Weifeng Teng Yong Luo Xiaochun Ji |
| author_sort | Gaoxiang Chen |
| collection | DOAJ |
| description | Cuprotosis is a recently identified cell death form that caused by intracellular copper accumulation and regulated by FDX1. This work aimed to explore the role of cuprotosis and the pivotal regulatory gene FDX1 in thyroid cancer development. We observed that expression of FDX1 in tumor section was notably lower than that in non-tumor sections in clinical samples. Induction of cuprotosis by elesclomol (ES) significantly repressed the in vitro and in vivo growth of thyroid cancer cells, simultaneously elevated Cu level and expression of FDX1, whereas depletion of FDX1 abolished these effects. Knockdown of FDX1 decreased the lipoylation level of DLAT and DLST in thyroid cancer cells, alleviated cuprotosis-induced cell death, simultaneously upregulated the levels of PA and α-KG. These findings demonstrated that FDX1 promotes the cuprotosis of thyroid cancer cells via regulating the lipoylation of DLAT. |
| first_indexed | 2024-03-12T12:21:33Z |
| format | Article |
| id | doaj.art-46f2e2ce088e443c967ab19147b08ed2 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T12:21:33Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-46f2e2ce088e443c967ab19147b08ed22023-08-30T05:52:06ZengElsevierHeliyon2405-84402023-08-0198e18655FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosisGaoxiang Chen0Jianan Zhang1Weifeng Teng2Yong Luo3Xiaochun Ji4Corresponding author.; Department of Thyroid and Breast Surgery, Ningbo Medical Center Lihuili Hospital, No.57 XingNing Road, Ningbo, Zhejiang Province, ChinaDepartment of Thyroid and Breast Surgery, Ningbo Medical Center Lihuili Hospital, No.57 XingNing Road, Ningbo, Zhejiang Province, ChinaDepartment of Thyroid and Breast Surgery, Ningbo Medical Center Lihuili Hospital, No.57 XingNing Road, Ningbo, Zhejiang Province, ChinaDepartment of Thyroid and Breast Surgery, Ningbo Medical Center Lihuili Hospital, No.57 XingNing Road, Ningbo, Zhejiang Province, ChinaDepartment of Thyroid and Breast Surgery, Ningbo Medical Center Lihuili Hospital, No.57 XingNing Road, Ningbo, Zhejiang Province, ChinaCuprotosis is a recently identified cell death form that caused by intracellular copper accumulation and regulated by FDX1. This work aimed to explore the role of cuprotosis and the pivotal regulatory gene FDX1 in thyroid cancer development. We observed that expression of FDX1 in tumor section was notably lower than that in non-tumor sections in clinical samples. Induction of cuprotosis by elesclomol (ES) significantly repressed the in vitro and in vivo growth of thyroid cancer cells, simultaneously elevated Cu level and expression of FDX1, whereas depletion of FDX1 abolished these effects. Knockdown of FDX1 decreased the lipoylation level of DLAT and DLST in thyroid cancer cells, alleviated cuprotosis-induced cell death, simultaneously upregulated the levels of PA and α-KG. These findings demonstrated that FDX1 promotes the cuprotosis of thyroid cancer cells via regulating the lipoylation of DLAT.http://www.sciencedirect.com/science/article/pii/S2405844023058632Thyroid cancerCuprotosisFDX1DLATLipoylation |
| spellingShingle | Gaoxiang Chen Jianan Zhang Weifeng Teng Yong Luo Xiaochun Ji FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis Heliyon Thyroid cancer Cuprotosis FDX1 DLAT Lipoylation |
| title | FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| title_full | FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| title_fullStr | FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| title_full_unstemmed | FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| title_short | FDX1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| title_sort | fdx1 inhibits thyroid cancer malignant progression by inducing cuprotosis |
| topic | Thyroid cancer Cuprotosis FDX1 DLAT Lipoylation |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023058632 |
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