Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI)
BACKGROUND: Carbapenems show excellent activity against resistant uropathogens, and they are the antibiotics of choice for urinary tract infections (UTIs). The choice of carbapenem prescription is strongly influenced by antimicrobial susceptibility testing (AST) report. With the publication of recen...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2018-07-01
|
| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_176_17 |
| _version_ | 1818949147578859520 |
|---|---|
| author | Chinmoy Sahu Vidhi Jain Prabhakar Mishra Kashi Nath Prasad |
| author_facet | Chinmoy Sahu Vidhi Jain Prabhakar Mishra Kashi Nath Prasad |
| author_sort | Chinmoy Sahu |
| collection | DOAJ |
| description | BACKGROUND: Carbapenems show excellent activity against resistant uropathogens, and they are the antibiotics of choice for urinary tract infections (UTIs). The choice of carbapenem prescription is strongly influenced by antimicrobial susceptibility testing (AST) report. With the publication of recent AST guidelines by the European Committee on AST (EUCAST), we were curious to evaluate the difference in results between Clinical and Laboratory Standards Institute (CLSI) and the EUCAST guidelines for the interpretation of carbapenems.
METHODS: During a period of 1 year, midstream urine specimens received in the laboratory were cultured by conventional techniques and 2932 of them grew significant colony counts of Escherichia coli. Out of them, 501 E. coli isolates which were resistant to at least six first-line antibiotics were further subjected to second-line antimicrobials imipenem and meropenem, reported by E-tests (bioMerieux, France). The E-test results were interpreted by both CLSI 2016 and EUCAST 6.0 (2016) guidelines. Weighted kappa was used to determine absolute agreement, and McNemar’s Chi-square test was used to test the difference in proportions of susceptibility between two methods, respectively.
RESULTS: Taking CLSI guidelines as a gold standard, there was 100% sensitivity in a susceptible category by the EUCAST guidelines for both the carbapenems. Weighted kappa showed good and moderate agreement between them for imipenem and meropenem, respectively. However, McNemar Chi-square test in the nonsusceptible category between the two tests was 9.38% and 33.03% for imipenem and meropenem, respectively, and they were highly significant (P < 0.001).
Conclusions: A laboratory can follow EUCAST guidelines as well and the guidelines are more useful in urinary concentrated antibiotics such as carbapenems. Further other antibiotics need to be evaluated by both these guidelines. |
| first_indexed | 2024-12-20T08:58:04Z |
| format | Article |
| id | doaj.art-46f46b7f9d4d42e5b178c0ae91c5b9c1 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-20T08:58:04Z |
| publishDate | 2018-07-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-46f46b7f9d4d42e5b178c0ae91c5b9c12022-12-21T19:45:56ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262018-07-01100328929310.4103/JLP.JLP_176_17Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI)Chinmoy Sahu0Vidhi Jain1Prabhakar Mishra2Kashi Nath Prasad3Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, IndiaDepartment of Microbiology, Yashoda Superspeciality Hospital, Kaushambi, Uttar Pradesh, IndiaDepartment of Biostatistics and Health Informatics, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, IndiaDepartment of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, IndiaBACKGROUND: Carbapenems show excellent activity against resistant uropathogens, and they are the antibiotics of choice for urinary tract infections (UTIs). The choice of carbapenem prescription is strongly influenced by antimicrobial susceptibility testing (AST) report. With the publication of recent AST guidelines by the European Committee on AST (EUCAST), we were curious to evaluate the difference in results between Clinical and Laboratory Standards Institute (CLSI) and the EUCAST guidelines for the interpretation of carbapenems. METHODS: During a period of 1 year, midstream urine specimens received in the laboratory were cultured by conventional techniques and 2932 of them grew significant colony counts of Escherichia coli. Out of them, 501 E. coli isolates which were resistant to at least six first-line antibiotics were further subjected to second-line antimicrobials imipenem and meropenem, reported by E-tests (bioMerieux, France). The E-test results were interpreted by both CLSI 2016 and EUCAST 6.0 (2016) guidelines. Weighted kappa was used to determine absolute agreement, and McNemar’s Chi-square test was used to test the difference in proportions of susceptibility between two methods, respectively. RESULTS: Taking CLSI guidelines as a gold standard, there was 100% sensitivity in a susceptible category by the EUCAST guidelines for both the carbapenems. Weighted kappa showed good and moderate agreement between them for imipenem and meropenem, respectively. However, McNemar Chi-square test in the nonsusceptible category between the two tests was 9.38% and 33.03% for imipenem and meropenem, respectively, and they were highly significant (P < 0.001). Conclusions: A laboratory can follow EUCAST guidelines as well and the guidelines are more useful in urinary concentrated antibiotics such as carbapenems. Further other antibiotics need to be evaluated by both these guidelines.http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_176_17carbapenemclinical and laboratory standards instituteeuropean committee on antimicrobial susceptibility testinginterpretation |
| spellingShingle | Chinmoy Sahu Vidhi Jain Prabhakar Mishra Kashi Nath Prasad Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) Journal of Laboratory Physicians carbapenem clinical and laboratory standards institute european committee on antimicrobial susceptibility testing interpretation |
| title | Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) |
| title_full | Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) |
| title_fullStr | Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) |
| title_full_unstemmed | Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) |
| title_short | Clinical and laboratory standards institute versus European committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for Escherichia coli in urinary tract infection (UTI) |
| title_sort | clinical and laboratory standards institute versus european committee for antimicrobial susceptibility testing guidelines for interpretation of carbapenem antimicrobial susceptibility results for escherichia coli in urinary tract infection uti |
| topic | carbapenem clinical and laboratory standards institute european committee on antimicrobial susceptibility testing interpretation |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_176_17 |
| work_keys_str_mv | AT chinmoysahu clinicalandlaboratorystandardsinstituteversuseuropeancommitteeforantimicrobialsusceptibilitytestingguidelinesforinterpretationofcarbapenemantimicrobialsusceptibilityresultsforescherichiacoliinurinarytractinfectionuti AT vidhijain clinicalandlaboratorystandardsinstituteversuseuropeancommitteeforantimicrobialsusceptibilitytestingguidelinesforinterpretationofcarbapenemantimicrobialsusceptibilityresultsforescherichiacoliinurinarytractinfectionuti AT prabhakarmishra clinicalandlaboratorystandardsinstituteversuseuropeancommitteeforantimicrobialsusceptibilitytestingguidelinesforinterpretationofcarbapenemantimicrobialsusceptibilityresultsforescherichiacoliinurinarytractinfectionuti AT kashinathprasad clinicalandlaboratorystandardsinstituteversuseuropeancommitteeforantimicrobialsusceptibilitytestingguidelinesforinterpretationofcarbapenemantimicrobialsusceptibilityresultsforescherichiacoliinurinarytractinfectionuti |