Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi
In recent years, the incidence of infections caused by pathogenic fungi has increased globally, especially in immunocompromised patients, transplant patients, or patients undergoing chemotherapy in intensive care units. Due to the morbidity and rapid spread of infection to other organs, accurate, ra...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2023-10-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-19576-en.pdf |
| _version_ | 1797542593971617792 |
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| author | Robab Ebrahimi Barogh Iman Haghani Javad Javidnia Mahdi Abastabar |
| author_facet | Robab Ebrahimi Barogh Iman Haghani Javad Javidnia Mahdi Abastabar |
| author_sort | Robab Ebrahimi Barogh |
| collection | DOAJ |
| description | In recent years, the incidence of infections caused by pathogenic fungi has increased globally, especially in immunocompromised patients, transplant patients, or patients undergoing chemotherapy in intensive care units. Due to the morbidity and rapid spread of infection to other organs, accurate, rapid diagnosis and treatment of fungal infections is essential. Molecular methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) have significant superiority in sensitivity and specificity compared to conventional methods. The aim of this study is to point out the limitations of common diagnostic methods and factors affecting the sensitivity and specificity of PCR-RFLP molecular diagnostic test.
This article is a review study for which subject-related academic papers (before 2023) were collected and studied by searching the keywords of pathogenic fungi, and RFLP molecular diagnostic methods; in Persian databases as well as Latin electronic databases including Scopus, Web of Science, and PubMed .
Reviewing numerous articles showed that PCR-RFLP is a rapid, practical, and reliable method, which can be used in laboratories to determine the genotype of specific variants of fungal species isolated from clinical samples. However, there are several limitations for PCR-RFLP method demanding for more attention. The main limitations are the need for specific restriction enzymes and the difficulty of accurately determining single nucleotide polymorphisms (SNPs) in a specific diagnostic locus. |
| first_indexed | 2024-03-10T13:32:46Z |
| format | Article |
| id | doaj.art-46f4c85d5599422cad78d3882a077598 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-03-10T13:32:46Z |
| publishDate | 2023-10-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-46f4c85d5599422cad78d3882a0775982023-11-21T07:44:49ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792023-10-0133225244260Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic FungiRobab Ebrahimi Barogh0Iman Haghani1Javad Javidnia2Mahdi Abastabar3 PhD Student, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran Assistant Professor, Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran PhD in Medical Mycology, Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Department of Medical Mycology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran and Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran In recent years, the incidence of infections caused by pathogenic fungi has increased globally, especially in immunocompromised patients, transplant patients, or patients undergoing chemotherapy in intensive care units. Due to the morbidity and rapid spread of infection to other organs, accurate, rapid diagnosis and treatment of fungal infections is essential. Molecular methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) have significant superiority in sensitivity and specificity compared to conventional methods. The aim of this study is to point out the limitations of common diagnostic methods and factors affecting the sensitivity and specificity of PCR-RFLP molecular diagnostic test. This article is a review study for which subject-related academic papers (before 2023) were collected and studied by searching the keywords of pathogenic fungi, and RFLP molecular diagnostic methods; in Persian databases as well as Latin electronic databases including Scopus, Web of Science, and PubMed . Reviewing numerous articles showed that PCR-RFLP is a rapid, practical, and reliable method, which can be used in laboratories to determine the genotype of specific variants of fungal species isolated from clinical samples. However, there are several limitations for PCR-RFLP method demanding for more attention. The main limitations are the need for specific restriction enzymes and the difficulty of accurately determining single nucleotide polymorphisms (SNPs) in a specific diagnostic locus.http://jmums.mazums.ac.ir/article-1-19576-en.pdfrflpmolecular diagnostic techniquefungi |
| spellingShingle | Robab Ebrahimi Barogh Iman Haghani Javad Javidnia Mahdi Abastabar Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi Journal of Mazandaran University of Medical Sciences rflp molecular diagnostic technique fungi |
| title | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi |
| title_full | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi |
| title_fullStr | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi |
| title_full_unstemmed | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi |
| title_short | Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, A Rapid and Simple Method for Identification of Pathogenic Fungi |
| title_sort | polymerase chain reaction restriction fragment length polymorphism a rapid and simple method for identification of pathogenic fungi |
| topic | rflp molecular diagnostic technique fungi |
| url | http://jmums.mazums.ac.ir/article-1-19576-en.pdf |
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