A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (COVID-19) is an acute respiratory infection transmitted by droplets, aerosols, and contact. Controlling the spread of COVID-19 and developing effective decontamination options are urgent issues for the international com...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2022-04-01
|
| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00941-21 |
| _version_ | 1818141627497054208 |
|---|---|
| author | Hiroshi Ueki Mutsumi Ito Yuri Furusawa Seiya Yamayoshi Shin-ichiro Inoue Yoshihiro Kawaoka |
| author_facet | Hiroshi Ueki Mutsumi Ito Yuri Furusawa Seiya Yamayoshi Shin-ichiro Inoue Yoshihiro Kawaoka |
| author_sort | Hiroshi Ueki |
| collection | DOAJ |
| description | ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (COVID-19) is an acute respiratory infection transmitted by droplets, aerosols, and contact. Controlling the spread of COVID-19 and developing effective decontamination options are urgent issues for the international community. Here, we report the quantitative inactivation of SARS-CoV-2 in liquid and aerosolized samples by a state-of-the-art, high-power, AlGaN-based, single-chip compact deep-UV (DUV) light-emitting diode (LED) that produces a record continuous-wave output power of 500 mW at its peak emission wavelength of 265 nm. Using this DUV-LED, we observed a greater-than-5-log reduction in infectious SARS-CoV-2 in liquid samples within very short irradiation times (<0.4 s). When we quantified the efficacy of the 265-nm DUV-LED in inactivating SARS-CoV-2, we found that DUV-LED inactivation of aerosolized SARS-CoV-2 was approximately nine times greater than that of SARS-CoV-2 suspension. Our data demonstrate that this newly developed, compact, high-power 265-nm DUV-LED irradiation system with remarkably high inactivation efficiency for aerosolized SARS-CoV-2 could be an effective and practical tool for controlling SARS-CoV-2 spread. IMPORTANCE We developed a 265-nm high-power DUV-LED irradiation system and quantitatively demonstrated that the DUV-LED can inactivate SARS-CoV-2 in suspensions and aerosols within very short irradiation times. We also found that the inactivation effect was about nine times greater against aerosolized SARS-CoV-2 than against SARS-CoV-2 suspensions. The DUV-LED has several advantages over conventional LEDs and mercury lamps, including high power, compactness, and environmental friendliness; its rapid inactivation of aerosolized SARS-CoV-2 opens up new possibilities for the practical application of DUV-LEDs in high-efficiency air purification systems to control airborne transmission of SARS-CoV-2. |
| first_indexed | 2024-12-11T11:02:53Z |
| format | Article |
| id | doaj.art-46f7e629507f423abaa4d3323f8b106b |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-11T11:02:53Z |
| publishDate | 2022-04-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-46f7e629507f423abaa4d3323f8b106b2022-12-22T01:09:48ZengAmerican Society for MicrobiologymSphere2379-50422022-04-017210.1128/msphere.00941-21A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 AerosolsHiroshi Ueki0Mutsumi Ito1Yuri Furusawa2Seiya Yamayoshi3Shin-ichiro Inoue4Yoshihiro Kawaoka5Department of Virology, Institute of Medical Science, University of Tokyo, Tokyo, JapanDepartment of Virology, Institute of Medical Science, University of Tokyo, Tokyo, JapanDepartment of Virology, Institute of Medical Science, University of Tokyo, Tokyo, JapanDepartment of Virology, Institute of Medical Science, University of Tokyo, Tokyo, JapanAdvanced ICT Research Institute, National Institute of Information and Communications Technology (NICT), Kobe, JapanDepartment of Virology, Institute of Medical Science, University of Tokyo, Tokyo, JapanABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (COVID-19) is an acute respiratory infection transmitted by droplets, aerosols, and contact. Controlling the spread of COVID-19 and developing effective decontamination options are urgent issues for the international community. Here, we report the quantitative inactivation of SARS-CoV-2 in liquid and aerosolized samples by a state-of-the-art, high-power, AlGaN-based, single-chip compact deep-UV (DUV) light-emitting diode (LED) that produces a record continuous-wave output power of 500 mW at its peak emission wavelength of 265 nm. Using this DUV-LED, we observed a greater-than-5-log reduction in infectious SARS-CoV-2 in liquid samples within very short irradiation times (<0.4 s). When we quantified the efficacy of the 265-nm DUV-LED in inactivating SARS-CoV-2, we found that DUV-LED inactivation of aerosolized SARS-CoV-2 was approximately nine times greater than that of SARS-CoV-2 suspension. Our data demonstrate that this newly developed, compact, high-power 265-nm DUV-LED irradiation system with remarkably high inactivation efficiency for aerosolized SARS-CoV-2 could be an effective and practical tool for controlling SARS-CoV-2 spread. IMPORTANCE We developed a 265-nm high-power DUV-LED irradiation system and quantitatively demonstrated that the DUV-LED can inactivate SARS-CoV-2 in suspensions and aerosols within very short irradiation times. We also found that the inactivation effect was about nine times greater against aerosolized SARS-CoV-2 than against SARS-CoV-2 suspensions. The DUV-LED has several advantages over conventional LEDs and mercury lamps, including high power, compactness, and environmental friendliness; its rapid inactivation of aerosolized SARS-CoV-2 opens up new possibilities for the practical application of DUV-LEDs in high-efficiency air purification systems to control airborne transmission of SARS-CoV-2.https://journals.asm.org/doi/10.1128/msphere.00941-21COVID-19LEDSARS-CoV-2aerosolsdeep UV |
| spellingShingle | Hiroshi Ueki Mutsumi Ito Yuri Furusawa Seiya Yamayoshi Shin-ichiro Inoue Yoshihiro Kawaoka A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols mSphere COVID-19 LED SARS-CoV-2 aerosols deep UV |
| title | A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols |
| title_full | A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols |
| title_fullStr | A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols |
| title_full_unstemmed | A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols |
| title_short | A 265-Nanometer High-Power Deep-UV Light-Emitting Diode Rapidly Inactivates SARS-CoV-2 Aerosols |
| title_sort | 265 nanometer high power deep uv light emitting diode rapidly inactivates sars cov 2 aerosols |
| topic | COVID-19 LED SARS-CoV-2 aerosols deep UV |
| url | https://journals.asm.org/doi/10.1128/msphere.00941-21 |
| work_keys_str_mv | AT hiroshiueki a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT mutsumiito a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT yurifurusawa a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT seiyayamayoshi a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT shinichiroinoue a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT yoshihirokawaoka a265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT hiroshiueki 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT mutsumiito 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT yurifurusawa 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT seiyayamayoshi 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT shinichiroinoue 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols AT yoshihirokawaoka 265nanometerhighpowerdeepuvlightemittingdioderapidlyinactivatessarscov2aerosols |