In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications
Molecular recognition was utilized to fabricate bioinspired calcium phosphate (Ca-P) coating on bioabsorbable magnesium alloys through small biomolecules such as Vitamin C (VC). Ca-P and VC hybrid coating (Ca-PVC) was successfully fabricated on AZ31 Mg alloy. The surface morphology and chemical comp...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Corrosion Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266726692200024X |
| _version_ | 1798032348289171456 |
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| author | Xue-Mei Wang Guan-Jie Lu Lan-Yue Cui Cheng-Bao Liu M. Bobby Kannan Fen Zhang Shuo-Qi Li Yu-Hong Zou Rong-Chang Zeng |
| author_facet | Xue-Mei Wang Guan-Jie Lu Lan-Yue Cui Cheng-Bao Liu M. Bobby Kannan Fen Zhang Shuo-Qi Li Yu-Hong Zou Rong-Chang Zeng |
| author_sort | Xue-Mei Wang |
| collection | DOAJ |
| description | Molecular recognition was utilized to fabricate bioinspired calcium phosphate (Ca-P) coating on bioabsorbable magnesium alloys through small biomolecules such as Vitamin C (VC). Ca-P and VC hybrid coating (Ca-PVC) was successfully fabricated on AZ31 Mg alloy. The surface morphology and chemical composition of the coatings were investigated using SEM, XRD, and FTIR together with XPS. The results showed that the Ca-PVC coating was composed of bamboo leaf-like Ca-P particles with a thickness of about three times that of the Ca-P coating. The surface roughness of the Ca-PVC coating (1.12 ± 0.12 μm) was lower than that (3.14 ± 1.93 μm) of Ca-P coating, suggesting the formation of refined Ca-P particles resulting from the VC addition. The corrosion resistance of the coated samples was characterized via electrochemical polarization, impedance spectroscopy, and immersion hydrogen evolution tests. The cell toxicity of the coated samples was evaluated utilizing mouse MC3T3-E1 pre-osteoblasts. The charge transfer resistance (Rct) of the Ca-PVC coated alloy increased as compared to the bare and Ca-P coated alloy samples. The Ca-PVC coated alloy exhibited minimal corrosion current density (1.36 × 10−6 A cm−2), which is one order of magnitude lower in comparison to that of the Ca-P coated alloy. These results confirm that VC addition greatly enhanced the coating resistance on AZ31 Mg alloy. It was also noticed that the Ca-PVC coated samples rapidly induced the formation of apatite after immersion in Hank's solution. VC was mainly transformed to L-Threonic acid, which facilitated the nucleation process of the Ca-PVC coating and significantly increased the thickness, density, and bonding strength of the coating. With enhanced corrosion resistance property and excellent biocompatibility, Ca-PVC coating has great potential for application in biodegradable Mg-based alloys. |
| first_indexed | 2024-04-11T20:11:33Z |
| format | Article |
| id | doaj.art-46fccce73a294106a7ddea86d85850dc |
| institution | Directory Open Access Journal |
| issn | 2667-2669 |
| language | English |
| last_indexed | 2024-04-11T20:11:33Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Corrosion Communications |
| spelling | doaj.art-46fccce73a294106a7ddea86d85850dc2022-12-22T04:05:05ZengElsevierCorrosion Communications2667-26692022-06-0161628In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applicationsXue-Mei Wang0Guan-Jie Lu1Lan-Yue Cui2Cheng-Bao Liu3M. Bobby Kannan4Fen Zhang5Shuo-Qi Li6Yu-Hong Zou7Rong-Chang Zeng8Corrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaSchool of Engineering, University of Newcastle, Callaghan, New South Wales 2308, Australia; College of Science and Engineering, James Cook University, Townsville, Queensland 4811, AustraliaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaSchool of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCorrosion Laboratory for Light Metals, College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, China; Corresponding author.Molecular recognition was utilized to fabricate bioinspired calcium phosphate (Ca-P) coating on bioabsorbable magnesium alloys through small biomolecules such as Vitamin C (VC). Ca-P and VC hybrid coating (Ca-PVC) was successfully fabricated on AZ31 Mg alloy. The surface morphology and chemical composition of the coatings were investigated using SEM, XRD, and FTIR together with XPS. The results showed that the Ca-PVC coating was composed of bamboo leaf-like Ca-P particles with a thickness of about three times that of the Ca-P coating. The surface roughness of the Ca-PVC coating (1.12 ± 0.12 μm) was lower than that (3.14 ± 1.93 μm) of Ca-P coating, suggesting the formation of refined Ca-P particles resulting from the VC addition. The corrosion resistance of the coated samples was characterized via electrochemical polarization, impedance spectroscopy, and immersion hydrogen evolution tests. The cell toxicity of the coated samples was evaluated utilizing mouse MC3T3-E1 pre-osteoblasts. The charge transfer resistance (Rct) of the Ca-PVC coated alloy increased as compared to the bare and Ca-P coated alloy samples. The Ca-PVC coated alloy exhibited minimal corrosion current density (1.36 × 10−6 A cm−2), which is one order of magnitude lower in comparison to that of the Ca-P coated alloy. These results confirm that VC addition greatly enhanced the coating resistance on AZ31 Mg alloy. It was also noticed that the Ca-PVC coated samples rapidly induced the formation of apatite after immersion in Hank's solution. VC was mainly transformed to L-Threonic acid, which facilitated the nucleation process of the Ca-PVC coating and significantly increased the thickness, density, and bonding strength of the coating. With enhanced corrosion resistance property and excellent biocompatibility, Ca-PVC coating has great potential for application in biodegradable Mg-based alloys.http://www.sciencedirect.com/science/article/pii/S266726692200024XMagnesium alloyCa-P coatingDegradationVitamin CBiomaterial |
| spellingShingle | Xue-Mei Wang Guan-Jie Lu Lan-Yue Cui Cheng-Bao Liu M. Bobby Kannan Fen Zhang Shuo-Qi Li Yu-Hong Zou Rong-Chang Zeng In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications Corrosion Communications Magnesium alloy Ca-P coating Degradation Vitamin C Biomaterial |
| title | In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications |
| title_full | In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications |
| title_fullStr | In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications |
| title_full_unstemmed | In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications |
| title_short | In vitro degradation and biocompatibility of vitamin C loaded Ca-P coating on a magnesium alloy for bioimplant applications |
| title_sort | in vitro degradation and biocompatibility of vitamin c loaded ca p coating on a magnesium alloy for bioimplant applications |
| topic | Magnesium alloy Ca-P coating Degradation Vitamin C Biomaterial |
| url | http://www.sciencedirect.com/science/article/pii/S266726692200024X |
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