Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection
(1) Background: Individuals with COVID-19 display different forms of disease severity and the upper respiratory tract microbiome has been suggested to play a crucial role in the development of its symptoms. (2) Methods: The present study analyzed the microbial profiles of the oral cavity and orophar...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2703 |
| _version_ | 1827639189097676800 |
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| author | William Bourumeau Karine Tremblay Guillaume Jourdan Catherine Girard Catherine Laprise |
| author_facet | William Bourumeau Karine Tremblay Guillaume Jourdan Catherine Girard Catherine Laprise |
| author_sort | William Bourumeau |
| collection | DOAJ |
| description | (1) Background: Individuals with COVID-19 display different forms of disease severity and the upper respiratory tract microbiome has been suggested to play a crucial role in the development of its symptoms. (2) Methods: The present study analyzed the microbial profiles of the oral cavity and oropharynx of 182 COVID-19 patients compared to 75 unaffected individuals. The samples were obtained from gargle screening samples. 16S rRNA amplicon sequencing was applied to analyze the samples. (3) Results: The present study shows that SARS-CoV-2 infection induced significant differences in bacterial community assemblages, with <i>Prevotella</i> and <i>Veillonella</i> as biomarkers for positive-tested people and <i>Streptococcus</i> and <i>Actinomyces</i> for negative-tested people. It also suggests a state of dysbiosis on the part of the infected individuals due to significant differences in the bacterial community in favor of a microbiome richer in opportunistic pathogens. (4) Conclusions: SARS-CoV-2 infection induces dysbiosis in the upper respiratory tract. The identification of these opportunistic pathogenic biomarkers could be a new screening and prevention tool for people with prior dysbiosis. |
| first_indexed | 2024-03-09T16:35:04Z |
| format | Article |
| id | doaj.art-46fe8f7f43cc479d869714ed38409fb1 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:35:04Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-46fe8f7f43cc479d869714ed38409fb12023-11-24T14:56:58ZengMDPI AGMicroorganisms2076-26072023-11-011111270310.3390/microorganisms11112703Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 InfectionWilliam Bourumeau0Karine Tremblay1Guillaume Jourdan2Catherine Girard3Catherine Laprise4Département des Sciences Fondamentales, Université du Québec à Chicoutimi, Saguenay, QC G7H 2B1, CanadaPharmacology-Physiology Department, Université de Sherbrooke, Saguenay, QC J1K 2R1, CanadaCentre Intersectoriel en Santé Durable, Université du Québec à Chicoutimi, Saguenay, QC G7H 2B1, CanadaDépartement des Sciences Fondamentales, Université du Québec à Chicoutimi, Saguenay, QC G7H 2B1, CanadaDépartement des Sciences Fondamentales, Université du Québec à Chicoutimi, Saguenay, QC G7H 2B1, Canada(1) Background: Individuals with COVID-19 display different forms of disease severity and the upper respiratory tract microbiome has been suggested to play a crucial role in the development of its symptoms. (2) Methods: The present study analyzed the microbial profiles of the oral cavity and oropharynx of 182 COVID-19 patients compared to 75 unaffected individuals. The samples were obtained from gargle screening samples. 16S rRNA amplicon sequencing was applied to analyze the samples. (3) Results: The present study shows that SARS-CoV-2 infection induced significant differences in bacterial community assemblages, with <i>Prevotella</i> and <i>Veillonella</i> as biomarkers for positive-tested people and <i>Streptococcus</i> and <i>Actinomyces</i> for negative-tested people. It also suggests a state of dysbiosis on the part of the infected individuals due to significant differences in the bacterial community in favor of a microbiome richer in opportunistic pathogens. (4) Conclusions: SARS-CoV-2 infection induces dysbiosis in the upper respiratory tract. The identification of these opportunistic pathogenic biomarkers could be a new screening and prevention tool for people with prior dysbiosis.https://www.mdpi.com/2076-2607/11/11/2703COVID-19SARS-CoV-2oropharynxoral cavitymicrobiomeMiniSeq |
| spellingShingle | William Bourumeau Karine Tremblay Guillaume Jourdan Catherine Girard Catherine Laprise Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection Microorganisms COVID-19 SARS-CoV-2 oropharynx oral cavity microbiome MiniSeq |
| title | Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection |
| title_full | Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection |
| title_fullStr | Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection |
| title_full_unstemmed | Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection |
| title_short | Bacterial Biomarkers of the Oropharyngeal and Oral Cavity during SARS-CoV-2 Infection |
| title_sort | bacterial biomarkers of the oropharyngeal and oral cavity during sars cov 2 infection |
| topic | COVID-19 SARS-CoV-2 oropharynx oral cavity microbiome MiniSeq |
| url | https://www.mdpi.com/2076-2607/11/11/2703 |
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