Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup>
<i>Gentiana radix</i> is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed...
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| Format: | Article |
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/23/12/3222 |
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| author | Xin Lv Jian-Zhi Sun Shi-Zhao Xu Qian Cai Yu-Qiang Liu |
| author_facet | Xin Lv Jian-Zhi Sun Shi-Zhao Xu Qian Cai Yu-Qiang Liu |
| author_sort | Xin Lv |
| collection | DOAJ |
| description | <i>Gentiana radix</i> is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed <i>Gentiana radix</i>. Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from <i>Gentiana radix</i>, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of <i>Gentiana radix</i>. By comparing the structure and peak areas of chemical constituents in crude and wine-processed <i>Gentiana radix</i>, we found that some compounds in crude and wine-processed <i>Gentiana radix</i> were significantly different. |
| first_indexed | 2024-12-11T20:46:26Z |
| format | Article |
| id | doaj.art-46ff81b3ae6440b49df4d1770a5a461c |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T20:46:26Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-46ff81b3ae6440b49df4d1770a5a461c2022-12-22T00:51:20ZengMDPI AGMolecules1420-30492018-12-012312322210.3390/molecules23123222molecules23123222Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup>Xin Lv0Jian-Zhi Sun1Shi-Zhao Xu2Qian Cai3Yu-Qiang Liu4Department of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaDepartment of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaDepartment of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaDepartment of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaDepartment of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China<i>Gentiana radix</i> is used in traditional Chinese medicine and has functions of clearing heat and drying dampness, as well as purging liver and gallbladder fire. A highly sensitive and effective strategy for rapid screening and identification of target constituents has been developed by using ultra high-performance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap) in crude and wine-processed <i>Gentiana radix</i>. Based on the accurate mass measurement (<5 ppm), retention times, and MS fragmentation ions, 52 constituents were unambiguously or tentatively characterized from <i>Gentiana radix</i>, including 21 iridoids, 11 flavonoids, 19 xanthones, and a triterpenoid. This study demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of compounds in the complex systems of <i>Gentiana radix</i>. By comparing the structure and peak areas of chemical constituents in crude and wine-processed <i>Gentiana radix</i>, we found that some compounds in crude and wine-processed <i>Gentiana radix</i> were significantly different.https://www.mdpi.com/1420-3049/23/12/3222UHPLC-LTQ-Orbitrapiridoidsflavonoidsxanthonescharacteristic fragmentation pathwayswine-processing<i>Gentiana radix</i> |
| spellingShingle | Xin Lv Jian-Zhi Sun Shi-Zhao Xu Qian Cai Yu-Qiang Liu Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> Molecules UHPLC-LTQ-Orbitrap iridoids flavonoids xanthones characteristic fragmentation pathways wine-processing <i>Gentiana radix</i> |
| title | Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> |
| title_full | Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> |
| title_fullStr | Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> |
| title_full_unstemmed | Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> |
| title_short | Rapid Characterization and Identification of Chemical Constituents in <i>Gentiana radix</i> before and after Wine-Processed by UHPLC-LTQ-Orbitrap MS<sup>n</sup> |
| title_sort | rapid characterization and identification of chemical constituents in i gentiana radix i before and after wine processed by uhplc ltq orbitrap ms sup n sup |
| topic | UHPLC-LTQ-Orbitrap iridoids flavonoids xanthones characteristic fragmentation pathways wine-processing <i>Gentiana radix</i> |
| url | https://www.mdpi.com/1420-3049/23/12/3222 |
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