Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate
In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs) and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au) nanocomposites were modified onto the surface of a glass carbon electrode (GCE) via self-assembly. The nano...
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| Format: | Article |
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MDPI AG
2013-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/4/5286 |
| _version_ | 1828116322054045696 |
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| author | Xiangyou Wang Xia Sun Yaoyao Cao Ying Zhu |
| author_facet | Xiangyou Wang Xia Sun Yaoyao Cao Ying Zhu |
| author_sort | Xiangyou Wang |
| collection | DOAJ |
| description | In this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs) and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au) nanocomposites were modified onto the surface of a glass carbon electrode (GCE) via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab) and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD), ultraviolet and visible absorption spectroscopy (UV-vis) and scanning electron microscopy (SEM), respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3). The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication. |
| first_indexed | 2024-04-11T12:55:00Z |
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| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T12:55:00Z |
| publishDate | 2013-04-01 |
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| series | Sensors |
| spelling | doaj.art-46fffad6b5554675bbf01a368c9ef9f12022-12-22T04:23:06ZengMDPI AGSensors1424-82202013-04-011345286530110.3390/s130405286Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody ConjugateXiangyou WangXia SunYaoyao CaoYing ZhuIn this paper, an amperometric immunosensor for the detection of carbofuran was developed. Firstly, multiwall carbon nanotubes (MWCNTs) and graphene sheets-ethyleneimine polymer-Au (GS-PEI-Au) nanocomposites were modified onto the surface of a glass carbon electrode (GCE) via self-assembly. The nanocomposites can increase the surface area of the GCE to capture a large amount of antibody, as well as produce a synergistic effect in the electrochemical performance. Then the modified electrode was coated with gold nanoparticles-antibody conjugate (AuNPs-Ab) and blocked with BSA. The monoclonal antibody against carbofuran was covalently immobilized on the AuNPs with glutathione as a spacer arm. The morphologies of the GS-PEI-Au nanocomposites and the fabrication process of the immunosensor were characterized by X-ray diffraction (XRD), ultraviolet and visible absorption spectroscopy (UV-vis) and scanning electron microscopy (SEM), respectively. Under optimal conditions, the immunosensor showed a wide linear range, from 0.5 to 500 ng/mL, with a detection limit of 0.03 ng/mL (S/N = 3). The as-constructed immunosensor exhibited notable performance features such as high specificity, good reproducibility, acceptable stability and regeneration performance. The results are mainly due to the excellent properties of MWCNTs, GS-PEI-Au nanocomposites and the covalent immobilization of Ab with free hapten binding sites for further immunoreaction. It provides a new avenue for amperometric immunosensor fabrication.http://www.mdpi.com/1424-8220/13/4/5286amperometric immunosensorcarbofurangold nanoparticles-antibody conjugationmultiwall carbon nanotubesgraphene sheets-PEI-Au nanocomposites |
| spellingShingle | Xiangyou Wang Xia Sun Yaoyao Cao Ying Zhu Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate Sensors amperometric immunosensor carbofuran gold nanoparticles-antibody conjugation multiwall carbon nanotubes graphene sheets-PEI-Au nanocomposites |
| title | Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate |
| title_full | Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate |
| title_fullStr | Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate |
| title_full_unstemmed | Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate |
| title_short | Amperometric Immunosensor for Carbofuran Detection Based on MWCNTs/GS-PEI-Au and AuNPs-Antibody Conjugate |
| title_sort | amperometric immunosensor for carbofuran detection based on mwcnts gs pei au and aunps antibody conjugate |
| topic | amperometric immunosensor carbofuran gold nanoparticles-antibody conjugation multiwall carbon nanotubes graphene sheets-PEI-Au nanocomposites |
| url | http://www.mdpi.com/1424-8220/13/4/5286 |
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