Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital
The increasing rates of nosocomial infection associated with coagulase–negative staphylococci (CoNS) were the rationale for this study, aiming to categorize oxacillin–resistant CoNS species recovered from blood culture specimens of inpatients at the UNESP Hospital das Clínicas in Botucatu, Brazil, o...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1413867020300179 |
| _version_ | 1811223776268910592 |
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| author | Valéria Cataneli Pereira Letícia Calixto Romero Luiza Pinheiro-Hubinger Adilson Oliveira Katheryne Benini Martins Maria de Lourdes Ribeiro de Souza da Cunha |
| author_facet | Valéria Cataneli Pereira Letícia Calixto Romero Luiza Pinheiro-Hubinger Adilson Oliveira Katheryne Benini Martins Maria de Lourdes Ribeiro de Souza da Cunha |
| author_sort | Valéria Cataneli Pereira |
| collection | DOAJ |
| description | The increasing rates of nosocomial infection associated with coagulase–negative staphylococci (CoNS) were the rationale for this study, aiming to categorize oxacillin–resistant CoNS species recovered from blood culture specimens of inpatients at the UNESP Hospital das Clínicas in Botucatu, Brazil, over a 20–year period, and determine their sensitivity to other antimicrobial agents. The mecA gene was detected in 222 (74%) CoNS samples, and the four types of staphylococcal chromosomal cassette mec (SCCmec) were characterized in 19.4%, 3.6%, 54.5%, and 14.4% of specimens, respectively, for types I, II, III, and IV. Minimal inhibitory concentration (MIC) values to inhibit 50% (MIC50) and 90% (MIC90) of specimens were, respectively, 2 and >256 μL/mL for oxacillin, 1.5 and 2 μL/mL for vancomycin, 0.25 and 0.5 μL/mL for linezolid, 0.094 and 0.19 μL/mL for daptomycin, 0.19 and 0.5 μL/mL for quinupristin/dalfopristin, and 0.125 and 0.38 μL/mL for tigecycline. Resistance to oxacillin and tigecycline and intermediate resistance to quinupristin/dalfopristin were observed. Eight (2.7%) of all 300 CoNS specimens studied showed reduced susceptibility to vancomycin. Results from this study show high resistance rates of CoNS to antimicrobial agents, reflecting the necessity of using these drugs judiciously and controlling nosocomial dissemination of these pathogens. |
| first_indexed | 2024-04-12T08:38:27Z |
| format | Article |
| id | doaj.art-47035d1a61f6493ca0bb820cc6c5b275 |
| institution | Directory Open Access Journal |
| issn | 1413-8670 |
| language | English |
| last_indexed | 2024-04-12T08:38:27Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-47035d1a61f6493ca0bb820cc6c5b2752022-12-22T03:39:57ZengElsevierBrazilian Journal of Infectious Diseases1413-86702020-03-01242160169Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospitalValéria Cataneli Pereira0Letícia Calixto Romero1Luiza Pinheiro-Hubinger2Adilson Oliveira3Katheryne Benini Martins4Maria de Lourdes Ribeiro de Souza da Cunha5UNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, Brazil; UNOESTE-Universidade Oeste Paulista, Departamento de Microbiologia, São Paulo, SP, Brazil; Correspondence author.UNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, BrazilUNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, BrazilUNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, BrazilUNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, BrazilUNESP-Universidade Estadual Paulista, Instituto de Biociências de Boucatu, Departamento de Microbiologia e Imunologia, São Paulo, SP, BrazilThe increasing rates of nosocomial infection associated with coagulase–negative staphylococci (CoNS) were the rationale for this study, aiming to categorize oxacillin–resistant CoNS species recovered from blood culture specimens of inpatients at the UNESP Hospital das Clínicas in Botucatu, Brazil, over a 20–year period, and determine their sensitivity to other antimicrobial agents. The mecA gene was detected in 222 (74%) CoNS samples, and the four types of staphylococcal chromosomal cassette mec (SCCmec) were characterized in 19.4%, 3.6%, 54.5%, and 14.4% of specimens, respectively, for types I, II, III, and IV. Minimal inhibitory concentration (MIC) values to inhibit 50% (MIC50) and 90% (MIC90) of specimens were, respectively, 2 and >256 μL/mL for oxacillin, 1.5 and 2 μL/mL for vancomycin, 0.25 and 0.5 μL/mL for linezolid, 0.094 and 0.19 μL/mL for daptomycin, 0.19 and 0.5 μL/mL for quinupristin/dalfopristin, and 0.125 and 0.38 μL/mL for tigecycline. Resistance to oxacillin and tigecycline and intermediate resistance to quinupristin/dalfopristin were observed. Eight (2.7%) of all 300 CoNS specimens studied showed reduced susceptibility to vancomycin. Results from this study show high resistance rates of CoNS to antimicrobial agents, reflecting the necessity of using these drugs judiciously and controlling nosocomial dissemination of these pathogens.http://www.sciencedirect.com/science/article/pii/S1413867020300179CoNSMICQuinupristin/dalfopristinSCCmecVancomycinTigecycline |
| spellingShingle | Valéria Cataneli Pereira Letícia Calixto Romero Luiza Pinheiro-Hubinger Adilson Oliveira Katheryne Benini Martins Maria de Lourdes Ribeiro de Souza da Cunha Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital Brazilian Journal of Infectious Diseases CoNS MIC Quinupristin/dalfopristin SCCmec Vancomycin Tigecycline |
| title | Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| title_full | Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| title_fullStr | Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| title_full_unstemmed | Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| title_short | Coagulase-negative staphylococci: a 20-year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| title_sort | coagulase negative staphylococci a 20 year study on the antimicrobial resistance profile of blood culture isolates from a teaching hospital |
| topic | CoNS MIC Quinupristin/dalfopristin SCCmec Vancomycin Tigecycline |
| url | http://www.sciencedirect.com/science/article/pii/S1413867020300179 |
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