Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant
The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses to evolve with their host and with changes in their environment. We examined the role of one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution to the variability...
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MDPI AG
2021-09-01
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| Online Access: | https://www.mdpi.com/1999-4915/13/10/1895 |
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| author | Eduardo Aguirre Inés Beperet Trevor Williams Primitivo Caballero |
| author_facet | Eduardo Aguirre Inés Beperet Trevor Williams Primitivo Caballero |
| author_sort | Eduardo Aguirre |
| collection | DOAJ |
| description | The mechanisms generating variability in viruses are diverse. Variability allows baculoviruses to evolve with their host and with changes in their environment. We examined the role of one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPV-Mex1) contained two genetic variants that dominated over other variants in individual larvae that consumed high (ChinNPV-K) and low (ChinNPV-E) concentrations of inoculum. Studies on the ChinNPV-K variant indicated that it was capable of generating novel variation in a concentration-dependent manner. In cell culture, cells inoculated with high concentrations of ChinNPV-K produced OBs with the ChinNPV-K REN profile, whereas a high diversity of ChinNPV variants was recovered following plaque purification of low concentrations of ChinNPV-K virion inoculum. Interestingly, the ChinNPV-K variant could not be recovered from plaques derived from low concentration inocula originating from budded virions or occlusion-derived virions of ChinNPV-K. Genome sequencing revealed marked differences between ChinNPV-K and ChinNPV-E, with high variation in the ChinNPV-K genome, mostly due to single nucleotide polymorphisms. We conclude that ChinNPV-K is an unstable genetic variant that is responsible for generating much of the detected variability in the natural ChinNPV isolates used in this study. |
| first_indexed | 2024-03-10T06:09:19Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T06:09:19Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj.art-4703f30a7ca447d099f3ef564d1bc2042023-11-22T20:17:41ZengMDPI AGViruses1999-49152021-09-011310189510.3390/v13101895Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single VariantEduardo Aguirre0Inés Beperet1Trevor Williams2Primitivo Caballero3Institute for Multidisciplinary Research in Applied Biology, Universidad Pública de Navarra, 31006 Pamplona, SpainDepartamento de Investigación y Desarrollo, Bioinsectis SL, 31110 Noain, SpainInstituto de Ecología AC, Xalapa 91073, MexicoInstitute for Multidisciplinary Research in Applied Biology, Universidad Pública de Navarra, 31006 Pamplona, SpainThe mechanisms generating variability in viruses are diverse. Variability allows baculoviruses to evolve with their host and with changes in their environment. We examined the role of one genetic variant of Chrysodeixis includens nucleopolyhedrovirus (ChinNPV) and its contribution to the variability of the virus under laboratory conditions. A mixture of natural isolates (ChinNPV-Mex1) contained two genetic variants that dominated over other variants in individual larvae that consumed high (ChinNPV-K) and low (ChinNPV-E) concentrations of inoculum. Studies on the ChinNPV-K variant indicated that it was capable of generating novel variation in a concentration-dependent manner. In cell culture, cells inoculated with high concentrations of ChinNPV-K produced OBs with the ChinNPV-K REN profile, whereas a high diversity of ChinNPV variants was recovered following plaque purification of low concentrations of ChinNPV-K virion inoculum. Interestingly, the ChinNPV-K variant could not be recovered from plaques derived from low concentration inocula originating from budded virions or occlusion-derived virions of ChinNPV-K. Genome sequencing revealed marked differences between ChinNPV-K and ChinNPV-E, with high variation in the ChinNPV-K genome, mostly due to single nucleotide polymorphisms. We conclude that ChinNPV-K is an unstable genetic variant that is responsible for generating much of the detected variability in the natural ChinNPV isolates used in this study.https://www.mdpi.com/1999-4915/13/10/1895ChinNPVvariabilityprevalenceSNPsconcentration<i>Chrysodeixis includens</i> |
| spellingShingle | Eduardo Aguirre Inés Beperet Trevor Williams Primitivo Caballero Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant Viruses ChinNPV variability prevalence SNPs concentration <i>Chrysodeixis includens</i> |
| title | Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant |
| title_full | Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant |
| title_fullStr | Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant |
| title_full_unstemmed | Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant |
| title_short | Generation of Variability in <i>Chrysodeixis includens</i> Nucleopolyhedrovirus (ChinNPV): The Role of a Single Variant |
| title_sort | generation of variability in i chrysodeixis includens i nucleopolyhedrovirus chinnpv the role of a single variant |
| topic | ChinNPV variability prevalence SNPs concentration <i>Chrysodeixis includens</i> |
| url | https://www.mdpi.com/1999-4915/13/10/1895 |
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