Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia
<p>Abstract</p> <p>Background</p> <p>Pancreatic intraepithelial neoplasias (PanINs) are precursors of malignant pancreatic cancer, an ideal stage for early cancer detection. We applied quantitative proteomics to identify aberrantly elevated proteins in pancreatic juice...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-06-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/9/1/149 |
| _version_ | 1811286989371080704 |
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| author | de Rham Sarah Sahota Rob A Nelson Brad H Duan Xiaobo Pan Sheng Chen Ru Kozarek Richard A McIntosh Martin Brentnall Teresa A |
| author_facet | de Rham Sarah Sahota Rob A Nelson Brad H Duan Xiaobo Pan Sheng Chen Ru Kozarek Richard A McIntosh Martin Brentnall Teresa A |
| author_sort | de Rham Sarah |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Pancreatic intraepithelial neoplasias (PanINs) are precursors of malignant pancreatic cancer, an ideal stage for early cancer detection. We applied quantitative proteomics to identify aberrantly elevated proteins in pancreatic juice samples derived from patients with PanIN3.</p> <p>Results</p> <p>Twenty proteins were found elevated in all three PanIN juices by at least two-fold. Among these proteins, anterior gradient-2 (AGR2) was found to be 2-10 fold elevated in PanIN3 juice samples analyzed by quantitative proteomics. An ELISA assay was developed to evaluate AGR2 levels in 51 pancreatic juice samples and 23 serum samples from patients with pancreatic cancer, pre-malignant lesions (including PanIN3, PanIN2, Intraductal Papillary Mucinous Neoplasms (IPMNs)) and benign disease controls (including chronic pancreatitis). AGR2 levels in the pancreatic juice samples were found significantly elevated in patients with pre-malignant conditions (PanINs and IPMNs) as well as pancreatic cancer compared to control samples (p ≤ 0.03). By ROC analysis, the AGR2 ELISA achieved 67% sensitivity at 90% specificity in predicting PanIN3 juice samples from the benign disease controls.</p> <p>Conclusions</p> <p>These results suggest that elevation of AGR2 levels in pancreatic juice occurs in early pancreatic cancer progression and could be further investigated as a potential candidate juice biomarker for early detection of pancreatic cancer.</p> |
| first_indexed | 2024-04-13T03:10:37Z |
| format | Article |
| id | doaj.art-4703f77b53444f6fa15cda3bcccdbaaa |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-04-13T03:10:37Z |
| publishDate | 2010-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-4703f77b53444f6fa15cda3bcccdbaaa2022-12-22T03:05:05ZengBMCMolecular Cancer1476-45982010-06-019114910.1186/1476-4598-9-149Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasiade Rham SarahSahota Rob ANelson Brad HDuan XiaoboPan ShengChen RuKozarek Richard AMcIntosh MartinBrentnall Teresa A<p>Abstract</p> <p>Background</p> <p>Pancreatic intraepithelial neoplasias (PanINs) are precursors of malignant pancreatic cancer, an ideal stage for early cancer detection. We applied quantitative proteomics to identify aberrantly elevated proteins in pancreatic juice samples derived from patients with PanIN3.</p> <p>Results</p> <p>Twenty proteins were found elevated in all three PanIN juices by at least two-fold. Among these proteins, anterior gradient-2 (AGR2) was found to be 2-10 fold elevated in PanIN3 juice samples analyzed by quantitative proteomics. An ELISA assay was developed to evaluate AGR2 levels in 51 pancreatic juice samples and 23 serum samples from patients with pancreatic cancer, pre-malignant lesions (including PanIN3, PanIN2, Intraductal Papillary Mucinous Neoplasms (IPMNs)) and benign disease controls (including chronic pancreatitis). AGR2 levels in the pancreatic juice samples were found significantly elevated in patients with pre-malignant conditions (PanINs and IPMNs) as well as pancreatic cancer compared to control samples (p ≤ 0.03). By ROC analysis, the AGR2 ELISA achieved 67% sensitivity at 90% specificity in predicting PanIN3 juice samples from the benign disease controls.</p> <p>Conclusions</p> <p>These results suggest that elevation of AGR2 levels in pancreatic juice occurs in early pancreatic cancer progression and could be further investigated as a potential candidate juice biomarker for early detection of pancreatic cancer.</p>http://www.molecular-cancer.com/content/9/1/149 |
| spellingShingle | de Rham Sarah Sahota Rob A Nelson Brad H Duan Xiaobo Pan Sheng Chen Ru Kozarek Richard A McIntosh Martin Brentnall Teresa A Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia Molecular Cancer |
| title | Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia |
| title_full | Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia |
| title_fullStr | Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia |
| title_full_unstemmed | Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia |
| title_short | Elevated level of anterior gradient-2 in pancreatic juice from patients with pre-malignant pancreatic neoplasia |
| title_sort | elevated level of anterior gradient 2 in pancreatic juice from patients with pre malignant pancreatic neoplasia |
| url | http://www.molecular-cancer.com/content/9/1/149 |
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