Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition
Skin layers serve as a barrier against unexpected critical changes in the body due to environmental factors. Excessive ultraviolet (UV) B exposure increases the levels of age-related factors, leading to senescent cells and damaged skin tissues. Widely used as a dietary supplement, konjac (<i>A...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/9/2779 |
| _version_ | 1797553915839905792 |
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| author | Kyung Ho Choi Sung Tae Kim Bum Ho Bin Phil June Park |
| author_facet | Kyung Ho Choi Sung Tae Kim Bum Ho Bin Phil June Park |
| author_sort | Kyung Ho Choi |
| collection | DOAJ |
| description | Skin layers serve as a barrier against unexpected critical changes in the body due to environmental factors. Excessive ultraviolet (UV) B exposure increases the levels of age-related factors, leading to senescent cells and damaged skin tissues. Widely used as a dietary supplement, konjac (<i>Amorphophallus konjac</i>) glucomannan (KGM) has shown skin regeneration potential in patch or sheet form with anti-inflammatory or immunosuppressive effects. However, the ability of KGM to reconstitute senescent/damaged skin following UV radiation has not been explored. Here, we demonstrate that KGM alleviates skin damage by increasing the proportion of young cell populations in UVB-exposed senescent human epidermal primary melanocytes. Young cell numbers increased depending on KGM dosage, but the senescent cells were not removed. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis showed that mRNA and protein levels of age- and pigmentation-related factors decreased in a manner dependent on the rate at which new cells were generated. Moreover, an analysis of mRNA and protein levels indicated that KGM facilitated youth by increasing cell proliferation in UVB-damaged human fibroblasts. Thus, KGM is a highly effective natural agent for maintaining skin homeostasis by promoting the reconstitution of the dermal environment against UVB-induced acute senescence or skin damage. |
| first_indexed | 2024-03-10T16:24:20Z |
| format | Article |
| id | doaj.art-4706f46215244042ab20f86a03e2a4b4 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T16:24:20Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-4706f46215244042ab20f86a03e2a4b42023-11-20T13:24:23ZengMDPI AGNutrients2072-66432020-09-01129277910.3390/nu12092779Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced ConditionKyung Ho Choi0Sung Tae Kim1Bum Ho Bin2Phil June Park3Department of Applied Biology, Ajou University, 206 World Cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16499, KoreaDepartment of Pharmaceutical Engineering, Inje University, Gimhae-si, Gyeongsangnam-do 50834, KoreaDepartment of Applied Biology, Ajou University, 206 World Cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do 16499, KoreaAMOREPACIFIC R&D Center, 1920 Yonggu-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17074, KoreaSkin layers serve as a barrier against unexpected critical changes in the body due to environmental factors. Excessive ultraviolet (UV) B exposure increases the levels of age-related factors, leading to senescent cells and damaged skin tissues. Widely used as a dietary supplement, konjac (<i>Amorphophallus konjac</i>) glucomannan (KGM) has shown skin regeneration potential in patch or sheet form with anti-inflammatory or immunosuppressive effects. However, the ability of KGM to reconstitute senescent/damaged skin following UV radiation has not been explored. Here, we demonstrate that KGM alleviates skin damage by increasing the proportion of young cell populations in UVB-exposed senescent human epidermal primary melanocytes. Young cell numbers increased depending on KGM dosage, but the senescent cells were not removed. Real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot analysis showed that mRNA and protein levels of age- and pigmentation-related factors decreased in a manner dependent on the rate at which new cells were generated. Moreover, an analysis of mRNA and protein levels indicated that KGM facilitated youth by increasing cell proliferation in UVB-damaged human fibroblasts. Thus, KGM is a highly effective natural agent for maintaining skin homeostasis by promoting the reconstitution of the dermal environment against UVB-induced acute senescence or skin damage.https://www.mdpi.com/2072-6643/12/9/2779konjac glucomannanultraviolet Bhuman epidermal primary melanocyteshuman embryonic fibroblastssenescence |
| spellingShingle | Kyung Ho Choi Sung Tae Kim Bum Ho Bin Phil June Park Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition Nutrients konjac glucomannan ultraviolet B human epidermal primary melanocytes human embryonic fibroblasts senescence |
| title | Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition |
| title_full | Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition |
| title_fullStr | Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition |
| title_full_unstemmed | Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition |
| title_short | Effect of Konjac Glucomannan (KGM) on the Reconstitution of the Dermal Environment against UVB-Induced Condition |
| title_sort | effect of konjac glucomannan kgm on the reconstitution of the dermal environment against uvb induced condition |
| topic | konjac glucomannan ultraviolet B human epidermal primary melanocytes human embryonic fibroblasts senescence |
| url | https://www.mdpi.com/2072-6643/12/9/2779 |
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