Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands
For most plants transformation methods and regeneration protocols are readily available, leading to more complicated and differential research in plant science. The application of expression or RNAi constructs in biotechnology can lead to unwanted effects and detrimental phenotypes, which may cause...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Italian Society of Silviculture and Forest Ecology (SISEF)
2012-06-01
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| Series: | iForest - Biogeosciences and Forestry |
| Subjects: | |
| Online Access: | https://iforest.sisef.org/contents/?id=ifor0620-005 |
| _version_ | 1811338639330770944 |
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| author | Hinze A Becker D |
| author_facet | Hinze A Becker D |
| author_sort | Hinze A |
| collection | DOAJ |
| description | For most plants transformation methods and regeneration protocols are readily available, leading to more complicated and differential research in plant science. The application of expression or RNAi constructs in biotechnology can lead to unwanted effects and detrimental phenotypes, which may cause great problems in the regeneration and breeding of transgenic plants. To circumvent this set back we resort to the transformation of inactive constructs. After the creation and establishment of transgenic lines the construct is specifically activated and the effects on the plants can be studied in detail, even if the resulting phenotype has harmful consequences. The activation of the constructs is carried out by means of induced site-directed recombination. The application of an heat shock leads to the expression of a recombinase, which excises the spacer fragment and leads to the desired effect on the plants. The feasibility of those constructs could be shown for RNAi, induced expression and even marker excision and confinement approaches demonstrating the huge variability of the new construct design. |
| first_indexed | 2024-04-13T18:14:08Z |
| format | Article |
| id | doaj.art-470a5b20034a48a38478ce69707c29c1 |
| institution | Directory Open Access Journal |
| issn | 1971-7458 1971-7458 |
| language | English |
| last_indexed | 2024-04-13T18:14:08Z |
| publishDate | 2012-06-01 |
| publisher | Italian Society of Silviculture and Forest Ecology (SISEF) |
| record_format | Article |
| series | iForest - Biogeosciences and Forestry |
| spelling | doaj.art-470a5b20034a48a38478ce69707c29c12022-12-22T02:35:45ZengItalian Society of Silviculture and Forest Ecology (SISEF)iForest - Biogeosciences and Forestry1971-74581971-74582012-06-015113113710.3832/ifor0620-005620Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demandsHinze A0Becker D1Biocenter Klein Flottbek, EBBT, University of Hamburg, Ohnhorststr. 18, D-22609 Hamburg (Germany)Biocenter Klein Flottbek, EBBT, University of Hamburg, Ohnhorststr. 18, D-22609 Hamburg (Germany)For most plants transformation methods and regeneration protocols are readily available, leading to more complicated and differential research in plant science. The application of expression or RNAi constructs in biotechnology can lead to unwanted effects and detrimental phenotypes, which may cause great problems in the regeneration and breeding of transgenic plants. To circumvent this set back we resort to the transformation of inactive constructs. After the creation and establishment of transgenic lines the construct is specifically activated and the effects on the plants can be studied in detail, even if the resulting phenotype has harmful consequences. The activation of the constructs is carried out by means of induced site-directed recombination. The application of an heat shock leads to the expression of a recombinase, which excises the spacer fragment and leads to the desired effect on the plants. The feasibility of those constructs could be shown for RNAi, induced expression and even marker excision and confinement approaches demonstrating the huge variability of the new construct design.https://iforest.sisef.org/contents/?id=ifor0620-005Site-specific RecombinationCre/loxPFLP/FRTHeat Shock Inducible PromoterTissue Specific ExpressionRNAi |
| spellingShingle | Hinze A Becker D Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands iForest - Biogeosciences and Forestry Site-specific Recombination Cre/loxP FLP/FRT Heat Shock Inducible Promoter Tissue Specific Expression RNAi |
| title | Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands |
| title_full | Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands |
| title_fullStr | Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands |
| title_full_unstemmed | Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands |
| title_short | Next generation biotechnology: how sophisticated constructs lead to further insights and new approaches towards biotechnology’s demands |
| title_sort | next generation biotechnology how sophisticated constructs lead to further insights and new approaches towards biotechnology s demands |
| topic | Site-specific Recombination Cre/loxP FLP/FRT Heat Shock Inducible Promoter Tissue Specific Expression RNAi |
| url | https://iforest.sisef.org/contents/?id=ifor0620-005 |
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