Body Fat-Reducing Effects of Whey Protein Diet in Male Mice
This study investigated the mechanism of reducing body fat via whey protein diet. Pregnant mice were fed whey or casein, and their offspring were fed by birth mothers. After weaning at 4 weeks, male pups received the diets administered to their birth mothers (n = 6 per group). At 12 weeks of age, bo...
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| Format: | Article |
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MDPI AG
2023-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/10/2263 |
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| author | Kimitaka Nakazaki Nobuhiko Nagano Daichi Katayama Shoichi Shimizu Kengo Matsuda Wataru Tokunaga Ryoji Aoki Kazumasa Fuwa Ichiro Morioka |
| author_facet | Kimitaka Nakazaki Nobuhiko Nagano Daichi Katayama Shoichi Shimizu Kengo Matsuda Wataru Tokunaga Ryoji Aoki Kazumasa Fuwa Ichiro Morioka |
| author_sort | Kimitaka Nakazaki |
| collection | DOAJ |
| description | This study investigated the mechanism of reducing body fat via whey protein diet. Pregnant mice were fed whey or casein, and their offspring were fed by birth mothers. After weaning at 4 weeks, male pups received the diets administered to their birth mothers (n = 6 per group). At 12 weeks of age, body weight, fat mass, fasting blood glucose (FBG), insulin (IRI), homeostatic model assessment of insulin resistance (HOMA-IR), cholesterol (Cho), triglyceride (TG), the expression levels of lipid metabolism-related genes in liver tissues and metabolomic data of fat tissues were measured and compared between the groups. The birth weights of pups born were similar in the two groups. Compared to the pups in the casein group, at 12 weeks of age, pups in the whey group weighed less, had significantly lower fat mass, HOMA-IR and TG levels (<i>p</i> < 0.01, <i>p</i> = 0.02, <i>p</i> = 0.01, respectively), and significantly higher levels of the antioxidant glutathione and the anti-inflammatory 1-methylnicotinamide in fat tissues (<i>p</i> < 0.01, <i>p</i> = 0.04, respectively). No differences were observed in FBG, IRI, Cho levels (<i>p</i> = 0.75, <i>p</i> = 0.07, <i>p</i> = 0.63, respectively) and expression levels of lipid metabolism-related genes. Whey protein has more antioxidant and anti-inflammatory properties than casein protein, which may be its mechanism for reducing body fat. |
| first_indexed | 2024-03-11T03:26:18Z |
| format | Article |
| id | doaj.art-470decf93c2b44ac9883d8da188a4ae4 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T03:26:18Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-470decf93c2b44ac9883d8da188a4ae42023-11-18T02:43:54ZengMDPI AGNutrients2072-66432023-05-011510226310.3390/nu15102263Body Fat-Reducing Effects of Whey Protein Diet in Male MiceKimitaka Nakazaki0Nobuhiko Nagano1Daichi Katayama2Shoichi Shimizu3Kengo Matsuda4Wataru Tokunaga5Ryoji Aoki6Kazumasa Fuwa7Ichiro Morioka8Department of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanDepartment of Pediatrics and Child Health, Nihon University School of Medicine, Tokyo 173-8610, JapanThis study investigated the mechanism of reducing body fat via whey protein diet. Pregnant mice were fed whey or casein, and their offspring were fed by birth mothers. After weaning at 4 weeks, male pups received the diets administered to their birth mothers (n = 6 per group). At 12 weeks of age, body weight, fat mass, fasting blood glucose (FBG), insulin (IRI), homeostatic model assessment of insulin resistance (HOMA-IR), cholesterol (Cho), triglyceride (TG), the expression levels of lipid metabolism-related genes in liver tissues and metabolomic data of fat tissues were measured and compared between the groups. The birth weights of pups born were similar in the two groups. Compared to the pups in the casein group, at 12 weeks of age, pups in the whey group weighed less, had significantly lower fat mass, HOMA-IR and TG levels (<i>p</i> < 0.01, <i>p</i> = 0.02, <i>p</i> = 0.01, respectively), and significantly higher levels of the antioxidant glutathione and the anti-inflammatory 1-methylnicotinamide in fat tissues (<i>p</i> < 0.01, <i>p</i> = 0.04, respectively). No differences were observed in FBG, IRI, Cho levels (<i>p</i> = 0.75, <i>p</i> = 0.07, <i>p</i> = 0.63, respectively) and expression levels of lipid metabolism-related genes. Whey protein has more antioxidant and anti-inflammatory properties than casein protein, which may be its mechanism for reducing body fat.https://www.mdpi.com/2072-6643/15/10/2263antioxidant effectanti-inflammatory effectglutathione1-methylnicotinamidemetabolite analyses |
| spellingShingle | Kimitaka Nakazaki Nobuhiko Nagano Daichi Katayama Shoichi Shimizu Kengo Matsuda Wataru Tokunaga Ryoji Aoki Kazumasa Fuwa Ichiro Morioka Body Fat-Reducing Effects of Whey Protein Diet in Male Mice Nutrients antioxidant effect anti-inflammatory effect glutathione 1-methylnicotinamide metabolite analyses |
| title | Body Fat-Reducing Effects of Whey Protein Diet in Male Mice |
| title_full | Body Fat-Reducing Effects of Whey Protein Diet in Male Mice |
| title_fullStr | Body Fat-Reducing Effects of Whey Protein Diet in Male Mice |
| title_full_unstemmed | Body Fat-Reducing Effects of Whey Protein Diet in Male Mice |
| title_short | Body Fat-Reducing Effects of Whey Protein Diet in Male Mice |
| title_sort | body fat reducing effects of whey protein diet in male mice |
| topic | antioxidant effect anti-inflammatory effect glutathione 1-methylnicotinamide metabolite analyses |
| url | https://www.mdpi.com/2072-6643/15/10/2263 |
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