Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers
The formation of amyloid fibrils is considered to be one of the main causes for many neurodegenerative diseases, such as Alzheimer’s, Parkinson’s or Huntington’s disease. Current knowledge suggests that amyloid-aggregation represents a nucleation-dependent aggregation process in vitro, where a sigmo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-05-01
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| Series: | Polymers |
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| Online Access: | http://www.mdpi.com/2073-4360/8/5/178 |
| _version_ | 1811218699279925248 |
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| author | Sebastian Funtan Zhanna Evgrafova Juliane Adler Daniel Huster Wolfgang H. Binder |
| author_facet | Sebastian Funtan Zhanna Evgrafova Juliane Adler Daniel Huster Wolfgang H. Binder |
| author_sort | Sebastian Funtan |
| collection | DOAJ |
| description | The formation of amyloid fibrils is considered to be one of the main causes for many neurodegenerative diseases, such as Alzheimer’s, Parkinson’s or Huntington’s disease. Current knowledge suggests that amyloid-aggregation represents a nucleation-dependent aggregation process in vitro, where a sigmoidal growth phase follows an induction period. Here, we studied the fibrillation of amyloid β 1-40 (Aβ40) in the presence of thermoresponsive polymers, expected to alter the Aβ40 fibrillation kinetics due to their lower critical solution behavior. To probe the influence of molecular weight and the end groups of the polymer on its lower critical solution temperature (LCST), also considering its concentration dependence in the presence of buffer-salts needed for the aggregation studies of the amyloids, poly(oxazolines) (POx) with LCSTs ranging from 14.2–49.8 °C and poly(methoxy di(ethylene glycol)acrylates) with LCSTs ranging from 34.4–52.7 °C were synthesized. The two different polymers allowed the comparison of the influence of different molecular structures onto the fibrillation process. Mixtures of Aβ40 with these polymers in varying concentrations were studied via time-dependent measurements of the thioflavin T (ThT) fluorescence. The studies revealed that amyloid fibrillation was accelerated in, accompanied by an extension of the lag phase of Aβ40 fibrillation from 18.3 h in the absence to 19.3 h in the presence of the poly(methoxy di(ethylene glycol)acrylate) (3600 g/mol). |
| first_indexed | 2024-04-12T07:13:44Z |
| format | Article |
| id | doaj.art-4712538aabd64bf98971a9d50405f88e |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-04-12T07:13:44Z |
| publishDate | 2016-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-4712538aabd64bf98971a9d50405f88e2022-12-22T03:42:32ZengMDPI AGPolymers2073-43602016-05-018517810.3390/polym8050178polym8050178Amyloid Beta Aggregation in the Presence of Temperature-Sensitive PolymersSebastian Funtan0Zhanna Evgrafova1Juliane Adler2Daniel Huster3Wolfgang H. Binder4Faculty of Natural Science II, Martin-Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, D-06120 Halle (Saale), GermanyFaculty of Natural Science II, Martin-Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, D-06120 Halle (Saale), GermanyInstitute for Medical Physics and Biophysics, Leipzig University, Härtelstraße 16-18, D-04107 Leipzig, GermanyInstitute for Medical Physics and Biophysics, Leipzig University, Härtelstraße 16-18, D-04107 Leipzig, GermanyFaculty of Natural Science II, Martin-Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, D-06120 Halle (Saale), GermanyThe formation of amyloid fibrils is considered to be one of the main causes for many neurodegenerative diseases, such as Alzheimer’s, Parkinson’s or Huntington’s disease. Current knowledge suggests that amyloid-aggregation represents a nucleation-dependent aggregation process in vitro, where a sigmoidal growth phase follows an induction period. Here, we studied the fibrillation of amyloid β 1-40 (Aβ40) in the presence of thermoresponsive polymers, expected to alter the Aβ40 fibrillation kinetics due to their lower critical solution behavior. To probe the influence of molecular weight and the end groups of the polymer on its lower critical solution temperature (LCST), also considering its concentration dependence in the presence of buffer-salts needed for the aggregation studies of the amyloids, poly(oxazolines) (POx) with LCSTs ranging from 14.2–49.8 °C and poly(methoxy di(ethylene glycol)acrylates) with LCSTs ranging from 34.4–52.7 °C were synthesized. The two different polymers allowed the comparison of the influence of different molecular structures onto the fibrillation process. Mixtures of Aβ40 with these polymers in varying concentrations were studied via time-dependent measurements of the thioflavin T (ThT) fluorescence. The studies revealed that amyloid fibrillation was accelerated in, accompanied by an extension of the lag phase of Aβ40 fibrillation from 18.3 h in the absence to 19.3 h in the presence of the poly(methoxy di(ethylene glycol)acrylate) (3600 g/mol).http://www.mdpi.com/2073-4360/8/5/178amyloid βfibrillationlower critical solution temperaturethermoresponsive polymer |
| spellingShingle | Sebastian Funtan Zhanna Evgrafova Juliane Adler Daniel Huster Wolfgang H. Binder Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers Polymers amyloid β fibrillation lower critical solution temperature thermoresponsive polymer |
| title | Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers |
| title_full | Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers |
| title_fullStr | Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers |
| title_full_unstemmed | Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers |
| title_short | Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers |
| title_sort | amyloid beta aggregation in the presence of temperature sensitive polymers |
| topic | amyloid β fibrillation lower critical solution temperature thermoresponsive polymer |
| url | http://www.mdpi.com/2073-4360/8/5/178 |
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